1,721,194 research outputs found
Strategie per l'ottenimento di piante transgeniche di poppo che esprimono geni di sterilità sessuale
No full textAntisense RNA strategy applied to carrot top1a and b genes as a new tool for investigating the apoptotic response in plant cells
No full textComparative investigation of two nuclear isoforms of carrot DNA topoisomerase I:role in cell growth.
No full textAntisense down-regulation of carrot top1a and top1b genes coding for topoisomerases I leads to delayed entry into PCD induced by reactive oxygen species.
No full textNuclease-producing bacteria in soil cultivated with herbicide resistant transgenic white poplars
No full textThis study was carried out using soil cultivated, under greenhouse conditions, with transgenic white poplars expressing the
bar gene for tolerance to the Basta® herbicide. The occurrence of extracellular nucleolytic activity was monitored in soil samples collected
at four different times over a 26-month period. The fraction of nuclease producing bacteria (NPB) ranged from 62.5 to 100%
of the total culturable bacterial population. The DNA-methyl green plate assay allowed to distinguish five groups of bacteria showing
increasing levels of extracellular DNase activity. The NPB isolates were classified by 16S rDNA sequence analysis as members of the
Bacillus, Brevibacillus, Microbacterium, Pseudomonas and Stenotrophomonas genera. For each genus, NPB isolates were cultured in
liquid medium and the nucleolytic activity during different growth phases was monitored. Production of extracellular nucleases was
observed only during the mid-exponential growth phase of the Brevibacillus, Microbacterium and Stenotrophomonas isolates, while no
activity was evidenced for isolates classified within the Bacillus and Pseudomonas genera
Genetic transformation of Populus deltoides and P. x euramericana clones using Agrobacterium tumefaciens
No full textThe susceptibility of different Populus euramericana (Neva, PE68-022 x P. nigra, 71-060 x P. nigra) and P.
deltoides (PE68-022 x P. deltoides) clones to wild-type Agrobacterium tumefaciens strains (A281 and 82.139) was
evaluated in an inoculation experiment, and differences in the frequency of tumor formation (0-48%) were found.
Co-cultivation experiments demonstrated high transformation ability of oncogenic binary A. tumefaciens strains as
compared to disarmed strains. Using oncogenic binary strains, transgenic calluses were obtained from all tested
clones. The presence of acetosyringone did not influence the transformation frequency of the disarmed strains.
Co-inoculation experiments were performed using leaf discs and a bacterial suspension containing both wild-type
and disarmed strains. No positive effects on transformation efficiency were noticed in these conditions either. The
transformation of tumors and kanamycin resistant calluses was confirmed by DNA analysis
Molecular dynamics of seed priming at the crossroads between basic and applied research
Full text linkKey message: The potential of seed priming is still not fully exploited. Our limited knowledge of the molecular dynamics of seed pre-germinative metabolism is the main hindrance to more effective new-generation techniques. Abstract: Climate change and other recent global crises are disrupting food security. To cope with the current demand for increased food, feed, and biofuel production, while preserving sustainability, continuous technological innovation should be provided to the agri-food sector. Seed priming, a pre-sowing technique used to increase seed vigor, has become a valuable tool due to its potential to enhance germination and stress resilience under changing environments. Successful priming protocols result from the ability to properly act on the seed pre-germinative metabolism and stimulate events that are crucial for seed quality. However, the technique still requires constant optimization, and researchers are committed to addressing some key open questions to overcome such drawbacks. In this review, an update of the current scientific and technical knowledge related to seed priming is provided. The rehydration–dehydration cycle associated with priming treatments can be described in terms of metabolic pathways that are triggered, modulated, or turned off, depending on the seed physiological stage. Understanding the ways seed priming affects, either positively or negatively, such metabolic pathways and impacts gene expression and protein/metabolite accumulation/depletion represents an essential step toward the identification of novel seed quality hallmarks. The need to expand the basic knowledge on the molecular mechanisms ruling the seed response to priming is underlined along with the strong potential of applied research on primed seeds as a source of seed quality hallmarks. This route will hasten the implementation of seed priming techniques needed to support sustainable agriculture systems
Requirement for topo Ia or topo Ib expression for plant cell viability revealed by antisense RNA strategy
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