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    Ultrastructural study of mature sperm of<i>Pomphorhynchus laevis</i>Müller (Acanthocephala: Palaeacanthocephala), a fish parasite

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    Ultrastructural observations on matine sperm of Pomphorhynchus laevis (Müller, 1776), the dominant acanthocephalan parasite of fish from the River Brenta, were carried out by scanning (SEM) and transmission electron microscopy (TEM). Adult males of P. laevis were obtained from the digestive tract of Leuciscus cephalus (Pisces: Cyprinidae). The spermatozoon of P. laevis is inverted with respect to conventional sperm models; it is filiform and consists of an anterior flagellar apparatus and a posterior nucleocytoplasmic derivative. The latter includes a layer of condensed chromatin beneath the plasma membrane opposite to the flagellum, numerous large subspherical electron dense inclusions, glycogen deposits and a pentalaminar structure next to the flagellum. The nucleocytoplasmic derivative terminates caudally with a flat lamina devoid of the dense inclusions and glycogen deposits. Proceeding through the posterior lamina, the 9 + 2 axoneme progressively disorganizes. The ultrastructural ..

    Microscopic Characterization of the Mucous Cells and Their Mucin Secretions in the Alimentary Canal of the Blackmouth Catshark <i>Galeus melastomus</i> (Chondrichthyes: Elasmobranchii)

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    Sharks belong to the most primitive group of jawed vertebrates and have some special structural and functional features such as a cartilaginous skeleton, a spiral intestinal valve, and a rectal gland for osmoregulation. In January 2020, ten specimens of Galeus melastomus, the Blackmouth catshark, were collected from the Gulf of Asinara (North Sardinia, Italy) and the entire alimentary canal was studied using histochemical reactions to characterize the mucous cell types. In the alimentary canal of G. melastomus, mucous cells mainly secrete a mixture of acidic and neutral mucins. Of the acidic mucins, only the carboxylated type was present in mucous cells of the stomach, while the sulfated type predominated in the esophagus and the intestines. The use of lectins revealed a distribution of sugar residues in mucins related to cellular activities of the different regions of the catshark alimentary canal. The current study is the first report to characterize the intestinal mucous cells of G. melastomus and to provide quantitative data on their different populations in the alimentary canal
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