1,720,976 research outputs found

    A Korean-Japanese-Chinese aligned wordnet with shared semantic hierarchy

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    Ministry of Science and Technolog

    Isolation and characterization of novel halotolerant and/or halophilic denitrifying bacteria with versatile metabolic pathways for the degradation of trimethylamine

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    Four denitrifying bacteria capable of degrading trimethylamine under both aerobic and denitrifying conditions were newly isolated from coastal sediments and wastewater contaminated by marine water. All strains were in alpha-Proteobacteria. Strain GP43 was classified as a member of genus Paracoccus, and strain PH32, PH34 and GRP21 were novel organisms with remote phylogenetic position from other genus alpha-Proteobacteria. Among these four strains were the halophilic strains PH32, PH34 and GRP21, which did not grow in the absence of sodium chloride in culture medium. Cells grown under denitrifying conditions possessed trimethylamine dehydrogenase while cells grown aerobically possessed two different enzymes for oxidation of trimethylamine, trimethylamine dehydrogenase and trimethylamine monooxygenase. The newly isolated strain PH32, PH34 and GRP21 may be the first halophilic bacteria to degrade trimethylamine under denitrifying conditions. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved

    Herbaspirillum chlorophenolicum sp nov., a 4-chlorophenol-degrading bacterium

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    A 4-chlorophenol-degrading bacterial strain, formerly designated as a strain of Comamonas testosteroni, was reclassified as a member of the genus Herbaspirillum based on its phenotypic and chemotaxonomic characteristics, as well as phylogenetic analysis using 16S rDNA sequences. Phylogenetic inference based on 16S rDNA sequences showed that strain CPW301(T) clusters in a phylogenetic branch that contains Herbaspirillum species. 16S rDNA sequence similarity of strain CPW301(T) to species of the genus Herbaspirillum with validly published names is in the range 98.7-98.9%. Despite the considerably high 16S rDNA sequence similarity, strain CPW301(T) could be distinguished clearly from type strains of Herbaspirillum species with validly published names by DNA-DNA relatedness values, which were < 15.7%. The genomic DNA G + C content of strain CPW301(T) is 61.3 mol%. The predominant ubiquinone is Q-8 and the major cellular fatty acids are C-16:0 and Cyclo-C-17:0. The strain does not fix nitrogen and is not plant-associated. It is an aerobic rod with one unipolar flagellum. On the basis of these characteristics, a novel Herbaspirillum species, Herbaspirillum chlorophenolicum sp. nov., is proposed. The type strain of the novel species is strain CPW301(T) (= KCTC 12096(T) = IAM 15024(T))

    Chryseobacterium daecheongense sp nov., isolated from freshwater lake sediment

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    A novel nitrate-reducing bacterium, CPW406(T), was isolated from the sediment of a shallow, freshwater lake. The strain was a Gram-negative, non-motile, non-spore-forming rod, which formed yellow-pigmented colonies on nutrient agar and contained a polyamine pattern with sym-homospermidine as the major compound, MK-6 as the predominant menaquinone, 15: 0 iso and 17 : 0 iso, 3-OH as the major fatty acids and phosphatidylethanolamine and several unknown lipids in the polar lipid profile. The 16S rRNA gene sequence of strain CPW406(T) was found to be most similar to that of the type strain of Chryseobacterium defluvii (DSM 14219(T); 97(.)9 % similarity). However, DNA-DNA relatedness data and its phenotypic properties showed that strain CPW406(T) could be distinguished from all known Chryseobacterium soecies and thus represented a novel species, for which the name Chryseobacterium daecheongense sp. nov. is proposed; the type strain is CPW406(T) (=DSM 15235(T) =KCTC 12088(T))

    Anaerobic degradation of pyrrolidine and piperidine coupled with nitrate reduction

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    Biodegradability of secondary amines (pyrrolidine, piperidine, piperazine, morpholine, and thiomorpholine) under anaerobic conditions was examined in microbial consortia from six different environmental sites. The consortia degraded pyrrolidine and piperidine under denitrifying conditions. Enrichment cultures were established by repeatedly sub-culturing the consortia on pyrrolidine or piperidine in the presence of nitrate. The enrichments strictly required nitrate for the anaerobic degradation and utilized pyrrolidine or piperidine as a carbon, nitrogen, and energy source for their anaerobic growths. The anaerobic degradation of pyrrolidine and piperidine reduced nitrate to nitrogen gas, indicating that these anaerobic degradations were coupled with a respiratory nitrate reduction. (C) 2002 Elsevier Science Ltd. All rights reserved

    Isolation and characterization of a novel Pseudomonas sp., strain YG1, capable of degrading pyrrolidine under denitrifying conditions

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    A denitrifying bacterium, strain YG1, capable of degrading pyrrolidine under denitrifying conditions, was isolated. On the basis of phenotypic and phylogenetic characteristics, it was identified as a member of the genus Pseudomonas. During the anaerobic degradation of pyrrolidine, YG1 reduced a stoichiometric amount of nitrate to nitrogen gas, demonstrating that the degradation of pyrrolidine is coupled with respiratory nitrate reduction. YG1 also degraded pyrrolidine with a higher degradation rate under aerobic conditions than under denitrifying conditions. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies
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