1,720,995 research outputs found
Cocaine effects on generation of reactive oxygen species and DNA damage:formation of 8-hydroxydeoxyguanosine in active abusers
No full textCocaine abuse continues to be a major public health problem in the world. An upper numbers of individuals are initiating cocaine use with a stable rate of growth each year with an increasing number of people with cocaine related problems. Following cocaine oxidative pathways a ROS formation are generated. Oxidative stress has been demonstrated to play an important role in cocaine addiction and toxicity due to its oxidized metabolites produced by cytochrome P450 during cocaine biotransformation. The ROS induced genotoxicities include DNA damage, gene mutation, chromosome aberrations and micronuclei formation. 8-Hydroxy-2’-deoxyguanosine (8-OHdG) an oxidative modified DNA product, is the most representative product that may reflect oxidative damage induced by ROS. The present study was designed to investigate whether a systemic cocaine administration and its metabolism increase 8-OHdG production. Our findings clearly showed that cocaine promoted the ROS formation with significant increased of urinary 8-OHdG and MDA with a decreased of total scavenging capacity (TSC)
Pharmacokinetic of melphalan in pelvic "stop-flow" infusion with hemofiltration. A pilot study in patients with recurrent unresectable pelvic cancer
No full textA valited HPLC method with eletrochemical detection for simultaneous of 5-aminosalicylic acid and its metabolite in human plasma
No full textA high-performance liquid chromatographic method was developed, validated and applied to the simultaneous determination
of 5-aminosalicylic acid (5-ASA) and its acetylated metabolite (acetyl-5-ASA) in human plasma. The method involves
liquid–liquid extraction with methanol followed by isocratic reversed-phase chromatography on a Kromasil KR100 C18 column
with electrochemical detection. The recovery, selectivity, linearity, precision and accuracy of the method were evaluated from
spiked human plasma samples. The effects of mobile phase composition, buffer concentration, mobile phase pH and concentration
of organic modifiers on retention of 5-ASA, acetyl 5-ASA and internal standard were investigated. Limits’ of detection were 5 ng/
mL for 5-ASA and 10 ng/mL for acetyl-5-ASA, respectively. The method can be used for supporting therapeutical drug monitoring
and pharmacokinetic studies. Copyright © 2004 John Wiley & Sons, Ltd
Muscarinic and nicotinic receptors and the function of the cholinergic synapse in the rat's hippocampus: selective age dependent changes and therapeutic interventions
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The effect of selective agonists and antagonists of excitatory aminoacid receptors on dopaminergic and cholinergic neurotrasmission in awake rats: possible clinical implication
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