733 research outputs found
The Impact of Interaction Between Author and Audience Shown on the Example of Hideaki Anno's "Neon Genesis Evangelion"
No full textDie vorliegende Arbeit beschäftigt sich mit Hideaki Anno als Person, seinem Werk und wie das Fandom seines Werkes dieses schließlich direkt beeinflusst hat. Dazu wird folgende Forschungsfrage gestellt: Wie genau hat das Fandom das Werk Annos verändert? Diese wird Anhand einer Analyse der Metaebene durch die Verfasserin untersucht. Dabei dient die Serie Neon Genesis Evangelion (1995-1996) als Ausgangspunkt und die Neuauflage der Serie, die sogenannten Rebuild-Filme (2007-2020), als Vergleichsobjekt. Die Metaebene beschreibt dabei, wie sich ein Medium über die reale Welt und seinen eigenen Einfluss darauf äußer
Measurements of Morphological Changes in Tonda River Estuary
No full textThe purpose of this study is to clarify the relation between sediment transport from river and topographical changes of mouth-bar by field measurements in Tonda river, which is second grade river flowing in south of Wakayama prefecture. Upstream areas of Tonda river were damaged by slope failures due to heavy rainfall which was caused by Typhoon No. 12 in 2011, and a large amount of sediment was supplied into the river. In this study, we will conduct field observations using RTK-GNSS during the next few years in order to capture the transporting phenomena of the overabundant supplied sediment from upstream area to estuary and sea. In this paper, we reported our recent results of field measurements.The purpose of this study is to clarify the relation between sediment transport from river and topographical changes of mouth-bar by field measurements in Tonda river, which is second grade river flowing in south of Wakayama prefecture. Upstream areas of Tonda river were damaged by slope failures due to heavy rainfall which was caused by Typhoon No. 12 in 2011, and a large amount of sediment was supplied into the river. In this study, we will conduct field observations using RTK-GNSS during the next few years in order to capture the transporting phenomena of the overabundant supplied sediment from upstream area to estuary and sea. In this paper, we reported our recent results of field measurements
Field Observations on Water Temperature and Salinity Fields in Tanabe Bay in 2016
No full textThe annual intensive field observations have been conducted using Tanabe-Nakashima observation tower by research group of Shirahama oceanographic observatory in Tanabe bay, Wakayama prefecture. In this study, we have conducted CTD (Conductivity (salinity)-Temperature-Depth) measurements supplementarily in the same bay area during the same period as the annual intensive observations. Those CTD results showed seasonal variations of water temperature and salinity, such as thermocline and density stratification which were observed at many points in Tanabe bay and freshwater entering from neighboring rivers. This paper reports our observation activities in 2016 and shows CTD results and three-dimensional structures of water temperature and salinity based on observed data
Revenue maximizing markets for zero-day exploits
No full textMarkets for zero-day exploits (software vulnerabilities unknown to the vendor) have a long history and a growing popularity. We study these markets from a revenue-maximizing mechanism design perspective. We first propose a theoretical model for zero-day exploits markets. In our model, one exploit is being sold to multiple buyers. There are two kinds of buyers, which we call the defenders and the offenders. The defenders are buyers who buy vulnerabilities in order to fix them (e.g., software vendors). The offenders, on the other hand, are buyers who intend to utilize the exploits (e.g., national security agencies and police). Our model is more than a single-item auction. First, an exploit is a piece of information, so one exploit can be sold to multiple buyers. Second, buyers have externalities. If one defender wins, then the exploit becomes worthless to the offenders. Third, if we disclose the details of the exploit to the buyers before the auction, then they may leave with the information without paying. On the other hand, if we do not disclose the details, then it is difficult for the buyers to come up with their private valuations. Considering the above, our proposed mechanism discloses the details of the exploit to all offenders before the auction. The offenders then pay to delay the exploit being disclosed to the defenders.Mingyu Guo, B, Hideaki Hata, and Ali Baba
The discovery of SycO reveals a new function for type three secretion effector chaperones
No full textThe Type Three Secretion (T3S) system is a device used by many Gram-negative pathogens that allows bacteria to deliver effector proteins straight into the eukaryotic cell cytosol. These effectors interfere with various signaling pathways to subvert the host cell functions. The secretion machinery of the T3S system consist of a basal body spanning the bacterial inner and outer membrane followed by a stiff hollow needle outside the bacterium. The fully assembled secretion apparatus constitute a continuous hollow conduit that connects the bacteria to the eukaryotic target cell. After cell contact, virulence proteins -called effectors- are injected directly into the cytosol of the host cell via the T3S apparatus. Several effectors of the T3S system require the assistance of specific cytosolic chaperones to be efficiently exported. There are three classes of T3S chaperones. Effector proteins are assisted by Class I chaperones. Although Class I chaperones are well characterized, their main function is still a matter of controversy. In this thesis, we demonstrate that orf155 encodes a specific chaperone for the effector YopO that we called SycO. We showed that SycO enhances YopO secretion in vitro and is required for translocation of YopO into infected cells. By pulldown assay we demonstrated that residues 20 to 77 of YopO are required and sufficient for SycO binding. Using crosslinking experiments and size exclusion chromatography analysis, we determined the stoichiometry of purified SycO and YopO-SycO complexes. SycO alone forms dimers in solution and the YopO-SycO complex has a 1:2 stoichiometry. These results suggested that SycO is a typical chaperone of the Class I. YopO is a serine/theronine kinase that interacts with Rho and Rac and disrupts the cytoskeleton of the target cells. YopO has been shown to localize at the cell plasma-membrane. By transfection of YopO-EGFP hybrid proteins into HEK293T cells, we demonstrated that the chaperone-binding domain (CBD) coincides with the membrane localization domain of YopO. Nevertheless, the CBD was not needed for the kinase activity of YopO. By ultracentrifugation, we also showed that the CBD causes YopO aggregation in the bacteria, when SycO does not cover it. Further, we show that the CBD of YopE and YopT also caused aggregation in the bacteria in the absence of SycE and SycT respectively. YopE, YopT and T3S effectors in other systems also act at the membrane of the eukaryotic host cell. We propose a new hypothesis concerning the role of T3S chaperones. The sub-cellular localization domain of effectors is aggregation-prone and creates the need for a chaperone inside bacteria. We propose that masking such aggregation-prone localization domains may be a general function for type III effector chaperones
ON DESIGN OF RESISTIVE WAVE METER
No full textAlthough the resistive wave meter, which measures the electric resistance bet-ween two electrodes, may be regarded old fashioned, it is used frequently with suc-cess for many laboratory experiments. Morever, it is used fruitfully for measure-ment of the internal waves which are generated at the interface of fresh and saltwaters. This is the reason why the author describes the method of designing this typeof wave meter. The fundamental principle of design is led by analyzing the measur-ing circuit. Pratical examples of such designing by the author are shown herewith.Although the resistive wave meter, which measures the electric resistance bet-ween two electrodes, may be regarded old fashioned, it is used frequently with suc-cess for many laboratory experiments. Morever, it is used fruitfully for measure-ment of the internal waves which are generated at the interface of fresh and saltwaters. This is the reason why the author describes the method of designing this typeof wave meter. The fundamental principle of design is led by analyzing the measur-ing circuit. Pratical examples of such designing by the author are shown herewith
Local Scour and Sediment Sorting Around a Spur Dyke
No full text水制周辺の洗掘現象において, 水制高と河床の粒度分布が局所洗掘に与える影響, そして, 水制がもたらす河床表層の粒度分布変化と水制高の関係性について明らかにするために, 越流型および非越流型水制に対していくつかの混合砂河床の移動床実験を実施し, 考察を行った。実験結果より, 越流型水制の場合も非越流型と同様に, 河床粒度の幾何標準偏差が最大洗掘深評価に重要なパラメータと成り得ることが示され, そして非越流型水制は越流型に比べて水制頭部の下流域で顕著な洗掘傾向が見られることが明らかとなった。また, 越流状態の異なる流れ場の計測により, 水制が高いほど水制先端近傍の下降流が大きくなり, その影響で水制が高いほど水制頭部から下流に伸びる河床表層の粗粒化域の横断幅が広くなることが明らかとなった。This paper presented an experimental study on the impact of both spur dyke height and grain size distribution on the bed topography and bed surface composition around an impermeable spur dyke. The importance of governing parameter, i.e. the relative spur dyke height, which is inverse number of overtopping ratio, was emphasized through quantitative evidences. It was found in the current experiments that the spatial progress pattern of scour-deposition in the non-submerged and nearly non-submerged condition was different compared with full-submerged condition. Furthermore, in the submerged experiments using non-uniform sediment bed, it was found that the maximum scour depth decreased with the increased of the standard deviation as well as in non-submerged spur dyke experiments. The flow measurement around the spur dyke revealed the difference of three-dimensional flow structure under the different spur dyke submergences, and the relationship to the variation of the surface bed composition. It was also found that with the increase of the spur dyke height, the longitudinal coarse sediment region was expanded to transverse direction in the downstream region of spur dyke head.水制周辺の洗掘現象において, 水制高と河床の粒度分布が局所洗掘に与える影響, そして, 水制がもたらす河床表層の粒度分布変化と水制高の関係性について明らかにするために, 越流型および非越流型水制に対していくつかの混合砂河床の移動床実験を実施し, 考察を行った。実験結果より, 越流型水制の場合も非越流型と同様に, 河床粒度の幾何標準偏差が最大洗掘深評価に重要なパラメータと成り得ることが示され, そして非越流型水制は越流型に比べて水制頭部の下流域で顕著な洗掘傾向が見られることが明らかとなった。また, 越流状態の異なる流れ場の計測により, 水制が高いほど水制先端近傍の下降流が大きくなり, その影響で水制が高いほど水制頭部から下流に伸びる河床表層の粗粒化域の横断幅が広くなることが明らかとなった。This paper presented an experimental study on the impact of both spur dyke height and grain size distribution on the bed topography and bed surface composition around an impermeable spur dyke. The importance of governing parameter, i.e. the relative spur dyke height, which is inverse number of overtopping ratio, was emphasized through quantitative evidences. It was found in the current experiments that the spatial progress pattern of scour-deposition in the non-submerged and nearly non-submerged condition was different compared with full-submerged condition. Furthermore, in the submerged experiments using non-uniform sediment bed, it was found that the maximum scour depth decreased with the increased of the standard deviation as well as in non-submerged spur dyke experiments. The flow measurement around the spur dyke revealed the difference of three-dimensional flow structure under the different spur dyke submergences, and the relationship to the variation of the surface bed composition. It was also found that with the increase of the spur dyke height, the longitudinal coarse sediment region was expanded to transverse direction in the downstream region of spur dyke head
Impact Measurement of Social Impact Bonds: How to Promote Social Innovation and Transparency
No full textDevelopment of Low Alcoholic Beverage using Alcohol Fermentation of ^|^ldquo;Beni-koji^|^rdquo; Mold
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