2,415 research outputs found
The Tuber borchii fruiting body-specific protein TBF-1, is a novel lectin which interacts with associated Rhizobium species
No full textLectins, proteins that are able to bind carbohydrate structures, are typically involved in cell recognition mechanisms. We demonstrate here that TBF-1, the main soluble protein in the Tuber borchii Vittad. fruiting body, is a phase-specific lectin that is able selectively to bind the exopolysaccharides produced by ascoma associated Rhizobium spp. Characterization of TBF-1 was performed using both the protein purified from the truffles and the recombinant protein overexpressed in Escherichia coli. The two proteins exhibit the same hemagglutination activity toward rabbit red blood cells and the same sugar binding specificity. The discovery of lectin activity for TBF-1 led us to propose revising the protein name to ‘T. borchii fruiting body lectin 1’ with the acronym TBFL-1
Headspace solid-phase microextraction with gas chromatography and mass spectrometry in the investigation of volatile organic compounds in an ectomycorrhizae synthesis system
No full textEctomycorrhizae formation represents one of the most significant steps in the truffle life cycle and
is determined by a complex molecular signaling between two symbionts. In order to understand
the molecular pathway of ectomycorrhiza development, we focused on the signaling interaction
between the ectomycorrhizal fungus Tuber borchii Vittad. and the Tilia americana L. plant roots.
The medium of a pre-symbiotic (T. americana–T. borchii) in vitro system was analysed by headspace
solid-phase microextraction coupled with gas chromatography and mass spectrometry. In
total, 73 volatile organic compounds (VOCs) were identified. Twenty-nine of these VOCs were produced
only during the interaction phase between the two partners, leading to a hypothesis that
these molecules might act as molecular messengers in order to pilot the ectomycorrhizae formatio
Carbohydrate and nitrogen metabolism in the ectomycorrhizal fungus Tuber borchii Vittad.
No full textHEXOSE UPTAKE IN TUBER BORCHII VITTAD.: CLONING AND CHARACTERIZATION OF THE SUGAR TRANSPORTER TBhxt
No full textIdentification and characterization of the Tuber borchii D-mannitol dehydrogenase which defines a new subfamily within the polyol-specific medium chain dehydrogenases
No full textA novel NADP(+)-dependent D-mannitol dehydrogenase and the corresponding gene from the plant symbiotic ascomycete fungus Tuber borchii was identified and characterized. The enzyme, called TbMDH, is a homotetramer with two zinc atoms per subunit. It catalyzed both D-fructose reduction and D-mannitol oxidation, although it showed the highest substrate specificity and catalytic efficiency for D-fructose. Co-factor specificity was restricted to NADP(H) and the reaction proceeded via a sequential ordered Bi Bi mechanism. The carbon responsive transcriptional pattern showed that Tbmdh is up-regulated when mycelia are transferred to a culture medium containing D-mannitol or D-fructose. The phylogenetic analysis showed TbMDH to be the first example of a fungal D-mannitol-2-dehydrogenase belonging to the medium-chain dehydrogenase/reductases (MDRs). The enzyme identified a new group of proteins, most of them annotated in databases as hypothetical zinc-dependent dehydrogenases, forming a distinct subfamily among the polyol dehydrogenase family
Identification of Tuber borchii Vittad. mycelium proteins separated by two-dimensional polyacrylamide gel electrophoresis using amino acid analysis and sequence tagging
No full textMichele Taruffo: el magisterio y la obra ejemplares del genial procesalista «todoterreno»
No full textThe author reflects on Michele Taruffo’s extraordinarily important contribution to the renewal of both conventional procedural law scholarship and the theoretical background of law-court professionals. Taruffo’s contribution was achieved by means of introducing to mainstream culture in those circles the necessary knowledge of the underlying epistemic dimension, which was traditionally suppressed by the strictly legal one.El autor discurre acerca de la importantísima contribución de Michele Taruffo a la renovación del procesalismo convencional y del bagaje teórico de los profesionales de la jurisdicción, mediante la incorporación a la cultura dominante en tales medios del imprescindible conocimiento de la dimensión epistémica subyacente y tradicionalmente sofocada por la propiamente jurídica
Enolase from the ectomycorrhizal fungus Tuber borchii Vittad.: biochemical characterization, molecular cloning, and localization
No full textEnolase from Tuber borchii mycelium was purified to electrophoretical homogeneity using an anion-exchange and a gel permeation chromatography. Furthermore, the corresponding gene (eno-1) was cloned and characterized. The purified enzyme showed a higher affinity for 2-PGA (0.26mM) with respect to PEP; the stability and activity of enolase were dependent of the divalent cation Mg2+. T. borchii eno-1 has an ORF of 1323 bp coding for a putative protein of 440 amino acids and Southern blotting analysis revealed that the gene is present as a single copy in T. borchii. The enzymatic activity and the mRNA expression level evaluated in mycelia grown either in different carbon sources, in pyruvate or during starvation were the same in all the conditions tested, while biochemical and Northern blotting analyses performed with mycelia at different days of growth showed T. borchii eno-1 regulation in response to the growth phase. Finally, Western blotting analysis demonstrated that enolase is localized only in the cytosolic fraction confirming its important role in glycolysis
- …
