1,720,987 research outputs found
Isolation of cDNA clones corresponding to the Mr=150,000 subunit of chick type VI collagen.
No full textType VI collagen is a disulfide-bonded protein with an unusual structure in that the molecule contains three short triple-helical domains and very extended non-collagenous regions. The molecule is a heterotrimer composed in the chick of two polypeptides of similar apparent size in SDS-PAGE (Mr = 140- and 150,000) but different structure, and a third component that is much larger (Mr = 260,000) than the other two chains. We report here on the isolation of several overlapping cDNA clones from a chicken aorta mRNA expression library in the plasmid vector pEX1. Antibodies affinity purified onto the fusion proteins recognized the chick type VI collagen Mr = 150,000 subunit. Northern blots using the cDNA inserts from the above clones revealed a single RNA species of about 4,600 nucleotides sufficient to code for a protein with the size of the Mr = 150,000 subunit
Stable expression of chicken type VI collagen alpha1, alpha2, and alpha3 cDNAs in murine NIH/3T3 cells.
No full textAs a component of an extensive network of microfibrils interwoven with large collagen fibers and in close contact with cell surfaces, type VI collagen plays an important role in cell-matrix interactions. To investigate the behaviour of chicken type VI collagen chains in heterologous host cells as a means to understanding the pattern of assembly of this collagen, we transfected murine NIH/3T3 cells with cDNAs encoding chicken alpha 1(VI), alpha 2(VI) and alpha 3(VI) chains. Cell lines that constitutively expressed the individual chains were analyzed by metabolic labeling and immunoprecipitation with specific antibodies. No self-association was observed for either alpha 1(VI) or alpha 2(VI) chains which were secreted as monomeric polypeptides. Furthermore, neither the chicken alpha 1(VI) nor alpha 2(VI) chains associated with the endogenous murine chains to form chimeric chicken/murine heterotrimers. In contrast, chimeric chicken/murine heterotrimers were detected in cell lines transfected with chicken alpha 3(VI) cDNA. These chimeric forms appeared to be properly aligned since their triple helices were stable to pepsin digestion. In addition, the chimeric heterotrimers coassembled and gave rise to disulfide-linked type VI collagen molecules
Comparison between wrought and EBM Ti6Al4V machinability characteristics
No full textElectron Beam Melting (EBM) is attracting large interest among the manufacturers of surgical implants as a near-net shape technology. Titanium alloy Ti6Al4V is widely used in the biomedical field thanks to its high biocompatibility, corrosion resistance and mechanical properties. The chemistry and microstructural features of EBM Ti6Al4V indicate lower machinability in comparison with wrought Ti6Al4V. Aim of the paper is to present a comparison between the machinability of wrought and EBM Ti6Al4V in semi-finishing external turning, by quantifying the effects of the cutting speed and the feed rate. Tool wear, surface integrity, chip morphology and microstructural analysis have been used to compare and assess the machinability of Ti6Al4V delivered in the two conditions
- …
