1,721,000 research outputs found
Strategies and technologies for tissue engineering and reparative medicine
No full textSince 1994, the Italian Interuniversity Research Centre on Materials for Biomedical Engineerign (CIRMIB), has been organizing an annual school on biomaterials and related topics. The aim of the 13th School was to illustrate principles, methodologies and applications of materials to the biomedical field, with particular attention to available technologies and recognized or emerging strategies that are able to trigger and drive tissue engineering and reparative-medicine processes. Selected speakers gave lessons on subjects within these areas
Surface modification of polyurethane scaffolds with natural polymers: the use of silk fibroin
No full textNew perspectives in cell delivery systems for tissue regeneration: natural-derived injectable hydrogels
No full textSilk fibroin/poly(carbonate)-urethane as a substrate for cell growth: in vitro interactions with human cells
No full textSilk fibroin (SF)-based or -coated biomaterials are likely to be endowed with structural and surface properties that render them
particularly apt for biomedical applications. In this work we investigated the behavior of four different strains of normal human
adult fibroblasts that had been seeded onto membranes made up of poly(carbonate) urethane (PCU), the surfaces of which had or
had not been homogeneously coated with SF. Cell adhesion within 3 h to the SF-coated PCU films was 2.2-fold that to their
uncoated homologues. After 30 days of incubation in vitro, 2.5-fold more cells had grown on the SF-coated specimens than on the
uncoated ones. This enhanced cell adherence and hence growth on the SF-coated surfaces was coupled with higher cumulative rates of d-glucose (but not l-glutamine) uptake and of bothlactate and interleukin-6 (IL-6) cumulative secretion. Conversely, human
fibroblasts cultured on either type of PCU scaffolds never secreted any ELISA-assayable amount of three main proinflammatory
cytokines, namely interleukin-1b (IL-1b), tumor necrosis factor-a (TNF-a), and transforming growthfactor- b1 (TGF-b1). Finally,
when the metabolic activities were compared on a per 105 cells basis, it became clear that the adhesion to SF favored an initially
higher consumption of d-glucose, a late higher release of IL-6, and an at-first more intense, but declining, extracellular assembly of type I collagen fibers. Overall, these results show that SF-coated PCU membranes represent a novel type of biomaterial that favors the adhesion, the growth and performance of specific metabolic tasks by normal human adult fibroblasts without eliciting any concurrent secretion of some of the chief proinflammatory cytokine
Funzionalizzazione organica di superfici di ossidi di metalli per applicazioni biomediche
No full textSILK FIBROIN/POLYURETHANE SCAFFOLDS: IN VITRO INTERACTIONS WITH HUMAN ADULT FIBROBLASTS
No full textStuttgart (Germany
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