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    High performance liquid chromatography separation of the intermediates of pyridine coenzymes metabolism in Ricinus communis L. seedlings

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    The metabolism of pyridine coenzymes is not yet well clarified in vegetable cells. We propose a HPLC method to separate the compounds involved in the pyridine nucleotides cycle in less than 32 min. The method has been applied to Ricinus communis seedlings. On the basis of the results obtained, the well known "de novo" and "salvage" pathways, demonstrated in other biological tissues for NAD biosynthesis, are likely to occur in this vegetable system

    The Mechanisms of Interaction Between Furanochromones and Dna - A Heteronuclear Overhauser Effect Study On the Khellin-thymidine Model System

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    The furanochromones khellin and visnagin have been characterised by 1H and 13C mono- and bidimensional NMR spectroscopies. Their strong affinity with DNA was experimentally confirmed by the complete disappearance of the furano-chromones' NMR signals upon additions of DNA. An intermolecular interaction between furanochromones and the thymidyl moieties of DNA, stabilized by the formation of a hydrogen bond between the thymidyl NH hydrogen and the C O group of khellin or visnagin, is here proposed. This is suggested by the strong donor-acceptor behavior of these two molecular moieties, as pointed out by a selective 1H-13C Overhauser effect study of the khellin-thymidine model system
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