1,720,981 research outputs found
Grapevine Vein Necrosis: further data on etiology and diagnosis
No full textVein necrosis (VN), a virus-like disease latent in all European grapevine cultivars and in most American rootstock species and hybrids, induces necrosis of the veinlets on its specific indicator Vitis rupestris × Vitis berlandieri 110 R. Vein necrosis is very common in Tuscany, where, during an indexing trial, 38 out of 62 (61%) putative grapevine clones selected during sanitary improvement programmes in the last few years indexed positive on 110 Richter. The 62 accessions were checked by PCR with two different universal primers (13/14 and RSP5/RSP6) for the presence of Grapevine rupestris stem pitting-associated virus (GRSPaV) and with group specific primers (Groups I, II and III). Primers 5/6 were able to detect all sequence variants and a differential biological behavior was observed among molecular variants. Only group I was highly associated to symptoms expression, groups II an III were not correlated to vein necrosi
Study of the Relationship Between Grapevine Ruspestris Stem Pitting Associated Virus Molecular Variants and Vein Necrosis
No full textGrapevine rupestris stem pitting associated virus (GRSPaV), is the putative causal agent of rupestris stem pitting (RSP) a component of the rugose wood disease of grapevine. Recently, a strikingly high association has been detected between GRSPaV and vein necrosis (VN), suggesting that 110 Richter (110 R) is the best indicator of GRSPaV infection. In the present study, we investigate the occurrence of molecular variants of GRSPaV in grapevine accessions previously indexed for vein necrosis on 110 R.
100% of the VN infected accessions results positive to GRSPaV by RT-PCR using primers 13/14. Using group specific primers, group I was detected in all the 22 accessions infected with VN, 11 times in mix infection with group III, 3 times with group II, and twice as a single infection. Unexpected results were observed with group II, in fact this group is present in 6 accessions negative to VN and to GRSPaV with primers 13/14. No single infection was, however, recorded for group III, the only available indication is its exclusive presence in VN infected accessions. This work confirms the association between GRSPaV and vein necrosis
Grapevine rupestris stem pitting-associated virus isolate 5/1-1, 28 kDa coat protein gene, complete sequence
No full textGenbank accession number: HQ39672
Grapevine Rupestris Stem Pitting-associated Virus and Vein Necrosis disease: further data on the molecular characterization of biologically divergent GRSPaV isolates
No full textSanitary status of autochton minor wine grape varieties: an unusual diffusion of Arabis mosaic virus
No full textIn the period 2007-2008, a sanitary survey was conducted on 99 plants from 9 autochton minor wine grape varieties selected in Garfagnana (LU, Italy). Sanitary status was verified by ELISA for 8 viruses (ArMV, GFLV, GLRaV 1, 2, 3, GVA, GVB and GFkV).
The serological assay pointed out a very high diffusion of ArMV (56.6%). This results is very unusual if correlated with previous research, conducted in Tuscany and in other Italian region
Molecular characterization of biologically divergent strains of GRSPaV
No full textThe comparison between results of biological indexing for vein necrosis and molecular analysis permits to elucidate the biological behaviour of GRSPaV molecular variants. Interestingly, expression of vein necrosis symptoms is likely to be restricted to infection with subgroups 2a and 2b. Moreover, clones isolated from vein necrosis affected isolates share more than 90% sequence identity among each other and share less than 84% sequence identity with the latent groups 1 and 3
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