16,719 research outputs found
Isoelectric focusing of protein and peptides in gel slab and in capillaries.
No full textThe present review offers a survey of modern isoelectric focusing (IEF) techniques, including the conventional one in soluble amphoteric buffers (CA-IEF) and immobilized pH gradients (IPG). In the case of CA-IEF, recent advances on its use as a first dimension for two-dimensional electrophoresis are covered. The properties of isoelectric buffers, in terms of buffering power and conductivity, are reviewed in view of their importance as background electrolytes in capillary zone electrophoresis (CZE). In the case of IPGs, examples are given of the high correlation between theoretically predicted and experimentally measured pi values. New aspects of IPGs, such as focusing in extremely alkaline pH intervals, and its interfacing with mass spectrometry, are described. In the case of capillary IEF, new aspects of the technique are described, in particular: (a) how to modulate the slope of the pH gradient for increasing resolution; and (b) how to keep proteins in solution at, and in the proximity of, the pi value. The review ends with an excursus on the use of isoelectric buffers in capillary zone electrophoretic separations. Such buffers offer unique advantages: they permit very high voltage gradients (up to 1000 V/cm) and, thus, minimize analysis times (down to a few minutes in 30-35 cm long capillaries). This results in a marked increment in resolution due to minimal diffusion-driven peak spreading. Such buffers are finding unique applications for generating peptide maps of tryptic digests of proteins and also in the analysis of intact proteins
Título: Opera
No full textCopia digital : Google BooksSign.: []\p3\s, *\p2\s, 2*\p1\s, A-Z\p4\s, 2A-2Z\p4\s, 3A-3M\p4\sPortada con escudo real calcográficoPort. con esc. calcLas hojas de grabado son tres retratos calcográficos : "Ant. Carnicero del., Ferd. Selma sculp.", retrato de Carlos IV, "Bon. Salesa delin, Cm. Carmona sculp.", retrato de Cicerón y "Bon. Salesa del., Jac. Bossi in.", retrato de Q. Hortensius"Las h. de grab. calc. : "Ant. Carnicero del., Ferd. Selma sculp.", retrato de Carlos IV precede a port., "Bon. Salesa delin, Cm. Carmona sculp.", retrato de Cicerón antes de p.1 y "Bon. Salesa del., Jac. Bossi in." , retrato de Q. Hortensius"antes de p. 15
Ultimo canto. Pensée musicale pour orchestre, tiré de l'op. 109, par M. Enrico Bossi. Partition.
No full textscore (19 p.) 28 cm
The politics and economics of regulatory impact assessment
Get PDFThis is the author accepted manuscript. The final version is available from the publisher via the link in this record
Concert (A moll) für Orgel, Streichorchester, vier Hörner und Pauken. Op. 100 von M. Enrico Bossi. Orgelstimme.
No full text1 part. 34 cm. Organ part only. Sibley Music Library copy bound with his Konzertstück, Op. 130 (1908
Capillary isoelectric focusing and isoelectric buffers: an evolving scenario.
No full textThe present review offers a new look at capillary isoelectric focusing (cIEF) by centering on the most troublesome aspects of the technique, namely: 1) how to modulate the slope of the pH gradient, for increasing resolution (equivalent to pH gradient engineering, as easily available in immobilized pH gradients); and 2) how to keep proteins in solution at (and in the proximity of) the pl value. A simple solution is offered in the first case: addition, to the standard 2-pH-units interval, of separators or spacers, i.e., of amphoteric molecules (either single or in combination) able to locally flatten the pH and increment resolution. Examples of the separation of fetal and glycated hemoglobins are provided. In the second case, a unique solubilization power (while maintaining full protein integrity and enzyme activity) is obtained if class I solubilizers are used. They consist of mixtures of sugars (e.g., sucrose and sorbitol) at ca. 1 M concentration, with zwitterions (up to 1 M) such as the class of nondetergent sulfobetaines, but also taurine and some of the Good's buffers (e.g., CAPS). In these solvents, the protein exists in a state of superhydration and its solubility is greatly augmented. The review ends with an excursus on the use of isoelectric buffers in zone electrophoretic separations. Such isoelectric buffers offer unique advantages: They permit very-high-voltage gradients (up to 1000 V/cm) and thus minimize analysis times (down to a few min in 30-35 cm long capillaries). This results in a marked increase in resolution, due to minimal diffusion-driven peak spreading. Such buffers are finding unique applications for generating peptide maps of tryptic digests of proteins and also in the analysis of oligonucleotides
Narrow-band UVB phototherapy and psoralen–ultraviolet A photochemotherapy in the treatment of cutaneous mastocytosis: a study in 20 patients
No full textBackground: In mastocytosis, the skin is almost invariably involved, and cutaneous symptoms deeply affect patients' quality of life. Methods: A retrospective observational analysis of patients affected by cutaneous mastocytosis (CM) and indolent systemic mastocytosis (ISM) treated with phototherapy/photochemotherapy (PUVA or NB-UVB) has been conducted. For each patient, total numbers of PUVA or NB-UVB exposures, the cumulative UV dose (J/cm2), serum tryptase profile, and pruritus, before and after treatment, according to the visual analogue scale (VAS) were considered. Skin lesions of each patient were assessed, before and after treatment, according to a cutaneous scale score. Results: Twenty patients affected by CM and ISM were studied; in particular, 10 patients received NB-UVB therapy, and other 10 patients received PUVA. A statistically significant mean reduction of pruritus in both groups (P < 0.01) was observed. The number of treatments necessary to obtain symptom relief was significantly lower in the PUVA group, but the mean exposure dose was significantly higher, if compared to the NB-UVB group. Serum tryptase levels showed a downward trend. The cutaneous score improved in both groups. Limitations: This study was a retrospective study with a small sample size and without a control group. Conclusion: This work provides evidence that both NB-UVB and PUVA represent a safe and useful second-line therapy of the cutaneous symptoms in mastocytosis
Siciliana / di Pe. G.B. Martini ; trascrizione per organo de M.E. Bossi.
No full text9 p. of music ; 23 x 30 cm. Caption title. Most likely a movement from one of Martini’s keyboard sonatas; arr. for organ
Phase Distribution Efficiency of cm-Scale Ultrasonically Powered Receivers
No full textIn the domain of ultrasonically powered biomedical implants, there is an increasing interest in cm-scale ultrasonic receivers (RX). However, when a single-element transducer is used as the RX transducer, an uneven phase distribution across the RX area can significantly reduce the harvestable power. In this paper, we investigate the impact of lateral and angular misalignment on the acoustic field phase distribution across the RX surface. We show that, for a single-element RX transducer, lateral misalignment has minimal effect on the harvestable power, whereas even small angular misalignments can cause a considerable reduction, especially for larger RX sizes. We present a potential solution that consists of subdividing a large RX transducer (e.g. 20 × 20mm2) into smaller elements, which significantly improves power transfer efficiency by taking advantage of the smaller phase variation across the surface of each element. The trade-offs between achieving a minimum acceptable power transfer efficiency and managing the increased complexity in packaging and matching circuitry are also discussed.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Electronic Components, Technology and MaterialsBio-Electronic
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