1,720,965 research outputs found
The use of texture analysis in the vertebral morphometric study of a lordotic chub (Squalius cephalus L.)
La depurazione biologica in acquario.
L’acquario è fondamentalmente un sistema a ricircolo nel quale l’acqua, per rallentarne l’inevitabile degrado, deve subire unaserie di processi fra i quali spicca la filtrazione o depurazione biologica. In un’ottica di sistema è importante che il medico veterinario“acquarista” acquisisca le conoscenze di base utili alla comprensione dei fenomeni biochimici batterici e sinecologiciconnessi con i processi di degradazione della sostanza organica, di nitrificazione e denitrificazione disassimilativa, conoscenzenecessarie per una corretta gestione globale del sistema acquario.[...
Aspetti clinici, necroscopici ed eziopatogenetici in pesci rossi (Carassius auratus auratus Linnaeus, 1758) di acquario con alterazione dell’assetto idrostatico
La "nutrizione integrata" dei pesci d'acquario
La corretta gestione dell’alimentazione dei pesci d’acquario presuppone conoscenze che vadano oltre il solo aspetto nutrizionalee che tengano in debita considerazione le esigenze ecologiche del sistema acquario. I pesci immettono infatti i prodottidel loro catabolismo direttamente all’interno dell’ambiente acquatico in cui vivono. Se in natura ciò non rappresenta un problema,ben diverso è il discorso in un ambiente confinato quale è l’acquario mediamente deficitario per quanto riguarda le propriecapacità autodepurative. Risulta di fondamentale importanza che il veterinario interessato all’acquariofilia acquisisca le necessarieconoscenze di base (nutrizionali ed ecologiche) per gestire in maniera integrata l’alimentazione dei pesci d’acquario e siain grado di trasferirle adeguatamente ai clienti acquariofili.[...
Valutazione dell'impatto sul sistema acquario derivante dall'uso di mangimi per pesci ornamentali
Cutaneous myxidiosis in European eel,Anguilla anguilla(Linnaeus, 1758): histopathology, histochemistry and laminin immunohistochemistry
Histopathology, histochemistry and immunohistochemistry of the integument of European eel, Anguilla anguilla (Linnaeus, 1758), infected by Myxidium sp. are reported. Skin samples from affected and unaffected eels were dissected, formalin fixed, paraffin embedded, sectioned and stained with H&E, Periodic acid–Schiff's staining method, Alcian Blue 8 GX pH 2.5/Periodic acid–Schiff's and McCallum-Goodpasture's Gram stain. Moreover, immunohistochemistry was performed using a primary polyclonal laminin antibody. Histologically, cysts (diameter 2–3 mm) were observed mainly under the scale pockets, encircled by a thin collagen layer, lined by elongated, flattened fibroblasts and containing bipolar, PAS- and Gram-positive spores with opposite polar capsules. The epidermis stretched by the underlying cyst appeared dysplastic, thinned with a significant reduction in mucous cells number. Only inconsistent and aspecific inflammatory reaction was noted around the cysts at the dermis/epidermis interface. Intense laminin-like protein immunolabel was documented in the plasmodial ectoplasm and related to host anergia. This was the first report of laminin immunolabel in a member of the Myxozoa. Epidermal dysplasia represents likely an aspecific response against the underlying tensile force exerted by the developing parasite cyst, while fibroblast and collagen encapsulation denote a parasite-driven host response protecting, rather than harming, the encircled parasite
Su di un caso di lordosi in un cavedano d'acquario (Leuciscus cephalus L, 1758)
The case of an aquarium European chub (Leuciscus cephalus L, 1758) is reported. The fish was introduced in aquarium atage class 0+ (approx standard length: 6 cm) where it was hosted, with goldfish (Carassius auratus auratus L, 1758), until itdied, because of circulation pump failure, at age class IV (standard length: 11.9 cm). Fish were regularly fed with goldfish formulatedfood. After a year, the chub developed progressive lordosis. When it died caudal tract was approximately perpendicularto the trunk. Morphometrics data were different from the expected values (obtained from bibliography) when the latter werecomputed with regard to the same age class. Spinal deformity was also characterised by means of radiograph examination.Lordosis began at the 29th vertebra and ended at the 34th vertebra, with a mild kyphosis starting at the level of the 35th vertebra.In the lordotic tract intervertebral space was reduced and vertebrae showed a trapezoidal shape rather than the normalrectangular shape. Nutrional imbalances due to feeding with inadequate food (with regard both with energy and protein contents)might have acted as predisposing factor sensitizing the axial skeleton to overload damage (due to intensive flashing)and buckling failure leading to lordosis as previously suggested in other fish species.[...
Assessment of mast cells degranulation in rainbow trout (Oncorhynchus mykiss Walbaum) by means of gray level and texture analysis (Gray Level Correlation Matrices)
Degranulation of intestinal mast cells in rainbow trout (Oncorhynchus mykiss Walbaum) was studied by means of image analysis technique. Two strips from the same intestinal segment from ten clinically healthy trout were sampled. One was immediately mounted in an organ bath, treated with compound 48/80 and then processed, as the remnant untreated strip, for light microscopy. Colour pictures taken from each section were converted in their gray levels RGB stacks equivalent and in 8 bits gray levels. Five granular cytoplasm areas of mast cells (MCs) for each section were analysed for the following parameters: mean gray values and Gray Level Correlation Matrices parameters. All RGB channels and the 8 bit gray levels transformed images showed higher mean gray level values after compound 48/80 exposure (Anova, p < 0.01). Only two channels (Red and Green) and the 8 bit gray levels transformed images of a unique texture parameter (contrast) appeared to be significantly higher after compound 48/80 exposure (Anova, p < 0.05). Red channel and Blue channel means gray level values were able to discriminate between not degranulated and degranulated MCs. The described model represents a promising alternative in mast cell research.[...
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