1,720,993 research outputs found
Opere matematiche della pubblica Biblioteca di Ferrara (1753-1815), éd. M. T. Borgato, L. Capra, A. Fiocca, L. Pepe
No full textOpere matematiche della pubblica Biblioteca di Ferrara (1753-1815), éd. M. T. Borgato, L. Capra, A. Fiocca, L. Pepe. In: Revue d'histoire des sciences, tome 37, n°3-4, 1984. pp. 360-361
Opere matematiche della pubblica Biblioteca di Ferrara (1753-1815), éd. M. T. Borgato, L. Capra, A. Fiocca, L. Pepe
No full textOpere matematiche della pubblica Biblioteca di Ferrara (1753-1815), éd. M. T. Borgato, L. Capra, A. Fiocca, L. Pepe. In: Revue d'histoire des sciences, tome 37, n°3-4, 1984. pp. 360-361
In vitro plant regeneration of the heavy metal tolerant and hyperaccumulator Arabidopsis halleri (Brassicaceae)
No full textPlants were regenerated from root explants of Arabidopsis halleri (L.) O'Kane and Al-Shehbaz via a three-step procedure callus induction, induction of somatic embryos and shoot development. Callus was induced from root segments, leaflets and petiole segments after incubation for 2 weeks in Murashige and Skoog medium (MS) supplemented with 0.5 mg/l -1 (2.26 μM) 2,4-D (2,4-dichlorophenoxyacetic acid) and 0.05 mg/l -1 (0.23 μM) kinetin. Only calli developed from root segments continued to grow when transferred to a regeneration medium containing 2.0 mg/l -1 (9.8 μM) 6-γ-γ-(dimethylallylamino)-purine (2ip) and 0.05 mg/l -1 (2.68 μM) α-naphthalenacetic acid (NAA) and eventually 40 of them developed embryogenic structures. On the same medium 38 of these calli regenerated shoots. Rooting was achieved for 50 of the shoots subcultured in MS medium without hormones. The regeneration ability of callus derived from root cuttings, observed in this study, makes this technique useful for genetic transformation experiments and in vitro culture studie
Somatic fusion between eggplant (Solanum melongena) and sexually incompatible arboreous Solanum species.
No full textfusion between Solanum melongena and arboreous Solanum specie
Plant regeneration from leaf protoplasts of Solanum virginianum L. (Solanaceae)
No full textLeaves of Solanum virginianum plants were used for protoplast isolation. To support cell wall formation and cell division, protoplasts were cultured in thin alginate layers floated in liquid medium. When protoplasts were plated at a density of 1.0 × 106/ml in Kao and Michyaluk (KMp8) medium supplemented with 0.5 mg/l zeatin, 1.0 mg/l 2,4-dichlorophenoxyacetic acid, and 1.0 mg/l α-naphthaleneacetic acid, 42.3% of the dividing cells developed microcalli in 3-4 weeks. Shoot formation via organogenesis of protoplast-derived calli was achieved for 28% of calli transferred to solidified KMp8 medium supplemented with 2.0 g/l zeatin and 0.1 mg/l 3-indol acetic acid in about 2 weeks. Further shoot development was observed in Murashige and Skoog (MS) medium without growth regulators and roots were induced after transfer to MS medium containing 1.0 mg/l 3-indol butyric acid. Regenerated plants have normal morphology
Production and characterization of arboreous and fertile Solanum melongena + Solanum marginatum somatic hybrid plants
No full textIn crop plants the shift from being annuals to perennials may allow future agricultural systems requiring less energy inputs. The practicability of this was tested for Solanum melongena. Leaf protoplasts of S. melongena (2n = 2x = 24) and one of the related arborescent species Solanum marginatum (2n = 2x = 24) were electrofused and fertile somatic hybrids with arborescent habit regenerated. The magnetic cell sorter (MACS) technique was used for the selection of heterokaryons. The hybrid nature of 18 regenerated plants was assessed on the banding patterns generated by inter-simple sequence repeat PCR. When taken to maturity in the greenhouse, hybrids grew more vigorously compared to the parental species. Their morphological traits were intermediate between those of S. melongena and S. marginatum. Hybrids flowered and produced an average of 85% stainable viable pollen and fertile fruits. The somatic hybrids were maintained in the greenhouse for more than 3 years and continued to produce flowers developing into two types of fruits with plentiful seeds. Fruits were either striated green containing non-germinable seeds or yellow with fully germinable seeds. Their S1 progenies showed common features with S0 hybrids, including fertility and arborescent habit. Cytologically, somatic hybrids exhibited the expected chromosome number of 2n = 4x = 48, while chromosome pairing during microsporogenesis was associated with a low frequency of intergenomic pairing. It is concluded that an arborescent perennial species has been obtained by somatic hybridization. The usefulness of this species per se or in eggplant breeding will depend not only on the transmission of the arborescent habit to cultivated eggplant varieties, but also on the variability that should be created from backcrossing the S. melongena + S. marginatum hybrids to S. melongen
Transcription profile of genes induced by cadmium in Brassica juncea for phytoremediation
No full texttranscription analysis of genes induced by cadmium in Brassica junce
Targeted approaches to triple-negative breast cancer: current practice and future directions.
No full textTriple-negative breast cancer (TNBC), that is breast cancer which stains negatively at immunohistochemistry for estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor 2 (HER2), comprises a particularly aggressive subtype of breast cancer, with high rate of early local and distant relapse. TNBC have a demonstrated sensitivity to cytotoxic treatment regimens, but given the absence of HER2, ER and PR there is no benefit from hormonal therapy or trastuzumab. The lack of known specific molecular targets has promoted abundant research in order to find possible “vulnerabilities” in TNBC and the evaluation of novel biomarkers overcoming the traditional approach based on hormonal receptors and HER2-targeted therapy is one of the priorities in breast cancer research. Drugs under investigation can be broadly divided in four groups: (1) Agents that create DNA damage (i.e. cisplatin, cyclophosphamide); (2) Agents that inhibit poly (ADP-ribose) polymerase (PARP); (3) Tyrosin-kinase inhibitors and monoclonal antibodies; (4) Agents that inhibit downstream signals. Several preclinical and early phase clinical trials for the treatment or management of patients with triple-negative breast tumors are underway. Nonetheless, so far the major issue to deal with when trying to provide evidence for TNBC is the small numbers of the sample in the clinical studies and the retrospective nature of most of them. Future large studies could help in defining optimal treatment strategies for TNBC, both in the advanced setting as well as in the (neo) adjuvant setting
Study of two Arabidopsis genes modulated by cadmium
No full textstudy of two genes regulated by cadmiu
The retrotransposon-like CDT-1 from the resurrection plant Craterostigma plantagineum induces dehydration tolerance through a si RNA mechanism
No full textidentification of a retrotransposon reponsible fo rdesiccation toleranc
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