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Sialylation patterns of the mouse parotid secretory granules. Combined deacetylation, enzymatic degradation and lectin-gold binding
No full textWe investigated the lectin cytochemistry of control and sialidase-digested sections of the mouse parotid gland by postembedding techniques. PNA and DBA lectins were used and their affinity sites were localized by employing conjugates with horseradish peroxidase that then reacted with anti-horseradish peroxidase antibody and protein A-gold. Potassium hydroxide pretreatment also was used before sialidase/PNA and DBA binding to investigate sialic acid acetylation. Ultrathin sections were obtained from specimens embedded in the acrylic hydrophilic resin, Bioacryl. The acini of mouse parotid gland contained polymorphous secretory grannies differentially stained by the two lectins; the use of sialidase digestion and KOH deacetylation revealed that the sialic acids linked to beta-galactose are restricted to the electron-dense concentric areas of target granules, whereas the sialic acids linked to alpha-N-acetylgalactosamine contain C-4-acetylated groups and are preferentially located in rite electron-lucent regions of bizonal granule
On the fine structure and complex carbohydrate cytochemistry of the rabbit parotid gland.
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Lectin histochemistry for differentiating glycoconjugates secreted by the bovine submandibular gland
No full textQualitative and quantitative lectin histochemistry in rat oviduct. A study at optic and electronic level
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Sex related differences in sialoglycoconjugates in the mouse submandibular gland (SMG) visualized by lectin-gold probes
No full textThe aim of this research I to determine, at the ultrastructural level, the localization and distribution of individual sugar sugar moieties and terminal sialylated sequences of the secretory products of the mouse SMG of both sexes. We therefore investigated the lectin receptors of control and sialidase-treated sections by a post-embedding approach using horse radish peroxidase (HRP)-conjugates (PNA, DBA, LTA, WGA, Con A), anti-HRP antibody and protein A-gold. Qualitative and quantitative differences occurred in the acinar products of males and females. Sialidase digestion revealed that the acceptor sugar for terminal sialic acids exhibit a different expression in the electron-lucent granules of acinar cells. The occurrence of terminal sialic acid-alpha-N-acetylgalactosamine disaccharide was prominent in male acinar cells in contrast to a modest presence in female. In addition, the terminal sequence sialic acid beta-galactose showed a homogeneous location in the male secretory products and a heterogeneous distribution in the paler areas of the electron-lucent granules in females. In agreement with our previous findings by light microscopy and confocal laser microscopy, the lectin-gold data seem to be correlated to a different expression of O- and N-linked oligosaccharides. These results provide further insight into the sexual dimorphism of the mouse SMG, previously though to be restricted to the convoluted granular tubules
Double labeling on the two faces of the same section with lectin-gold and silver enhancement. Ultrastructural detection of the terminal disaccharides sialic acid-beta-galactose and sialic acid-alpha-N-acetylgalactosamine
No full textThe double-labeling with protein A-gold performed on the two faces of the tissue sections requires protein A-gold complexes with different gold particle size. To avoid artifacts and steric hindrance phenomena related to the gold size, an approach was developed making use of protein A-gold complex of 10 nm on both faces and silver enhancement of gold signal on one side only. Present data also confirmed that among the polymorphous secretory granules of the mouse parotid gland, only the darker regions of the target granules secrete sialoglycoconjugates containing sialic acid linked to beta-galactose while the paler regions of bizonal granules secrete sialoglycoconjugates characterized by terminal sialic acid linked to alpha-D-N-acetylgalactosamine and containing C4 acetylated residue
Quantitation of lectin staining induced by glycosidases in the sucking cat submandibular gland
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