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LEPTIN INFLUENCES HISTIDINE DIPEPTIDES AND NITRIC OXIDE RELEASE FROM ANTERIOR PITUITARY CELLS OF SHEEP IN VITRO
INFLUENCE OF OVARECTOMY AND 17B-OESTRADIOL ON FIBROGENIC ACTIVITY OF RAT LIVER STELLATE CELLS EXPOSED TO KETOGENIC DIET
Test di tolleranza al glucosio (IVGT) e rilascio insulinico nel cavallo: influenza dell’età
The response of bile secretion and ubiquinone Q10 to hyperglycaemia in sheep
The aim of these investigations was to establish the secretion of ubiquinone Q10 (UQ10) in bile of sheep under glucose-induced cholestasis. Experiments were performed on 9 cannulated sheep divided into three groups: I-infused with sodium taurocholate, II-with Na-taurocholate plus glucose, III-with Na-taurocholate and glucose plus propranolol, phentolamine and atropine. Infusion of glucose increased plasma glucose concentration from 3.89 +/- 0.593 mM/l to 12.69 +/- 0.852 mM/l in 90 min and produced elevation of plasma insulin from 124.68 +/- 1.984 to 839.54 +/- 29.212 pM/l. Employment of blocking agents reduced insulin release to maximum 685.71 +/- 50.087 pM/l in 90 min. Under infusion of Na-taurocholate, bile flow averaged 14.016 +/- 0.706 microl/min/kg b wt. In the second group, bile flow decreased to 7.08 +/- 0.59 microl/min/kg b wt. in 90 min, and reached 11.25 +/- 0.25 microl/min/kg b wt in 240 min. Addition of the blocking agents in the third group, resulted in a significant (p < 0.05) decrease in bile flow to 3.733 +/- 0.680 microl/min/kg b wt in 105 min. This reduction of bile flow occurred with significant (p < 0.05) reduction of bile acids secretion that averaged 0.032 +/- 0.087 mM/min/kg in the first hour after glucose infusion and was maintained to the end of the experiment. Marked (p < 0.05) increase in UQ10 secretion was observed in both experimental groups. Maximum values of UQ10 secretion were obtained during the second hour of the experiment and averaged 0.449 +/- 0.196ng/min/kg b wt in the second, and 0.338 +/- 0.184ng/min/kg b wt in the third group of animals. Because at the end of the experiment UQ10 secretion gradually decreased we have concluded that free radicals generated during cholestasis lead to reduction of endogenous antioxidant capacity
Prove preliminari di separazione di spermatozoi di coniglio e di cane su gradienti discontinui di albumina bovina.
Gallbladder pressure changes induced by caerulein, pilocarpine and bile salts in calves measured by radiotelemetry
Applications of the Stewart model for the analysis of acid-base disturbances in dogs with chronic renal insufficiency
The objective of these studies was to introduce the Stewart approach to analyses of acid-base changes in dogs with the chronic renal failure (CRF). The acid-base status was investigated in 12 healthy dogs and 20 dogs with CRF. In the CRF affected dogs the level of urea and creatinin rose to 350 mg/dl and 394.8 mu mol/l respectively. The three independence variables (P-CO2, SID-strog ion difference, and the A(tot)-sum of net charges of nonvolatile plasma buffers (albumin + inorganic phosphate) together with the strong ion gap (SIG) were calculated by a method adopted from articles employing Stewart's approach. The SID averaged 41.85 mEq/l and 48.81 mEq/1 in normal and diseased dogs respectively. Nonvolatile plasma buffers (A(tot)) were higher especially in more severely diseased dogs. The most considerable changes in affected dogs have been observed in SIG where, together with the increasing values of urea, the quantity of SIG successively dropped, attaining negative values (-28.03 mEq/l). The albumin values were significantly associated with the SID values. All affected dogs have metabolic acidosis partly compensated by respiratory alkalosis. As has been clearly observed the estimation of SIG may be used as an accurate marker of CRF in dogs
EVALUATIONS OF THE METABOLIC PATTERN OF TEPOXALIN AFTER SINGLE ORAL ADMINISTRATION IN THE HORSE
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