1,721,057 research outputs found
Demonstration of the dimeric structure of the mitochondrial oxoglutarate carrier by the formation of a disulfide cross-linking.
No full text
Mitochondrial citrate carrier protein: transported ionic species and reaction mechanism in proteoliposomes.
No full text
Expression in E. coli and reconstitution into liposomes of the mitochondrial oxoglutarate and phosphate carriers
No full textPurification of reconstitutively active tricarboxylate carrier from rat liver mitochondria
No full textIsolation of reconstitutively active dicarboxylate and oxoglutarate carriers from rat liver mitochondria
No full text
Role of FOXA and Sp1 in mitochondrial acylcarnitine carrier gene expression in different cell lines
No full textThis study investigates the transcriptional role of the human mitochondrial carnitine/acylcarnitine carrier
(CAC) proximal promoter. Through deletion analysis, an activation domain (334/80 bp) was identified
which contains FOXA and Sp1 active sites. The wild-type (but not mutated) 334/80 bp region of
the CAC gene conferred 74% LUC transgene activity in HepG2 cells, 17% in HEK293 cells and 14% in SK-NSH
cells as compared to that observed with the entire 1503/+3 bp proximal promoter. Overexpression
and silencing of FOXA2 or Sp1 in HepG2 cells enhanced and diminished, respectively, LUC activity, CAC
transcript and CAC protein. In HEK293 and SK-N-SH cells, which do not contain FOXA1-3, LUC activity
was increased by FOXA2 overexpression to a greater extent than in HepG2 cells. Both FOXA2 and Sp1
in HepG2, and only Sp1 in HEK293 and SK-N-SH cells, were found to be bound to the CAC proximal promoter.
These results show that FOXA and Sp1 sites in HepG2 cells and only the Sp1 site in HEK293 and
SK-N-SH cells have a critical role in the transcriptional regulation of the CAC proximal promoter
Transmembrane topography of the polypeptide chain of the mitochondrial oxoglutarate carrier
No full text- …
