1,720,962 research outputs found
Intracellular killing of Brucella melitensis within mouse peritoneal macrophages: influence of treatment with rifampicin. An ultrastructural study
No full textThe aggregation and condensation of ribosomes and the disjunction of the cell-wall membranous system are the ultrastructural alterations caused by rifampicin on B. melitensis cultured in Brucella-Broth medium. Our ultrastructural researches carried out on mouse peritoneal macrophages infected with B. melitensis and treated with rifampicin (1 microgram/ml) have demonstrated that vacuoles containing B. melitensis which had been damaged by the drug fuse with lysosomes. On the contrary vacuoles containing undamaged and viable Brucellae showed markedly impaired lysosomal fusion
Typhoid fever and leukocytic functions in man: "in vivo" inhibition of cell migration into expermentally indiced inflammation sites
No full textGli AA. espongono i risultati delle loro ricerche sulla citologia della risposta infiammatoria sterile mediante la tecnica della finestrella cutanea di Rebuck. La risposta cellulare in tali pazienti risulta riidotta se confrontata con quella di controlli sani e di pazienti febbrili per infezioni provate da gram-positivi. Solo la componente granulocitica è compromessa, ma non in maniera strettamente correlabile alla coincidente neutropenia del sangue periferico. I dati ottenuti vengono discussi alla luce delle conoscenze acquisite sulla azione delle ondotossine e sulla conetica dei leucociti nella risposta infiammatori
A comparative ultrastructural study of the low virulent strain PV-C1/81 and RH strain cultured in A9, Vero, SIRC cell lines
No full textA human Toxoplasma strain, isolated in mice from bioptic lymphoglandular tissue, has been cultured in vitro in continuous cell lines. Its presence in some parastized cells, with parasitophorous vacuoles surrounded by a cyst-like wall and filled with a cystozoite-like clone, was observed by electron microscopy. These characteristic and constant features were confined in different cell line cultures and are suggested as possible markers of low virulent strains
Intracellular killing of Brucella melitensis within mouse peritoneal macrophages: influence of treatment with rifampicin. An ultrastructural study
No full textThe aggregation and condensation of ribosomes and the disjunction of the cell-wall membranous system are the ultrastructural alterations caused by rifampicin on B. melitensis cultured in Brucella-Broth medium. Our ultrastructural researches carried out on mouse peritoneal macrophages infected with B. melitensis and treated with rifampicin (1 microgram/ml) have demonstrated that vacuoles containing B. melitensis which had been damaged by the drug fuse with lysosomes. On the contrary vacuoles containing undamaged and viable Brucellae showed markedly impaired lysosomal fusion
In vitro sensitivity of 3 strains of Leptospira interrogans to 3 different antibiotics
No full textLeptospirosis, anthropo-zoonosis ubiquitously widespread, is a social and economic problem still to be solved. The experimental and therapeutic employment of many antibiotics has largely been tested "in vitro" and "in vivo". In the following research we tried to evaluate, by experimental "in vitro" method, the sensitivity difference of three serovar strains of Leptospira interrogans to two macrolides. Erythromycin and Josamycin, compared with Penicillin. From standard cultures, previously treated with serial dilution of these antibiotics, the MIC and MSC, as quantitative parameters, have been stated. For the qualitative evaluation of the damages induced at ultrastructural level by the drug activity. Electron Microscopy investigations were performed on specimens from cultures treated for 6 hr with twice and tenfold the MSC. The present research confirms the good sterilizing efficaciousness "in vitro" of the tested macrolides (MSC less than 1 mcg/ml) and their different activity pathway
Toxoplasma gondii: in vivo and in vitro immunological and ultrastructural patterns of a human low virulent strain
No full textThe AA. analyze the ultrastructural and immunological patterns of a low virulent strain of T. gondii cultured in vitro on A-9 cells line (PV-H1C-83). The tissue culture method described here is highly recommended to study the immunological aspects of experimental toxoplasmosis. Biological tests' results and the time courses of serological tests are also similar to those obtained from mice infected with cerebral tissue containing cysts. The low virulent strain (PV-H1C-83), when cultured in vitro, is able to induce the formation of large parasitophorous vacuoles, in which the EM investigation shows a typical cyst wall enveloping numerous bradyzoites
Toxoplasma gondii: in vivo and in vitro immunological and ultrastructural patterns of a human low virulent strain
No full textThe AA. analyze the ultrastructural and immunological patterns of a low virulent strain of T. gondii cultured in vitro on A-9 cells line (PV-H1C-83). The tissue culture method described here is highly recommended to study the immunological aspects of experimental toxoplasmosis. Biological tests' results and the time courses of serological tests are also similar to those obtained from mice infected with cerebral tissue containing cysts. The low virulent strain (PV-H1C-83), when cultured in vitro, is able to induce the formation of large parasitophorous vacuoles, in which the EM investigation shows a typical cyst wall enveloping numerous bradyzoites
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