1,721,101 research outputs found
Top-down proteomics with a quadrupole time-of-flight mass spectrometer and collision-induced dissociation.
No full textMonoclonal antibodies to human erythrocyte glucose 6-phosphate dehydrogenase
No full textSeveral antisera human erythrocyte glucose 6-phosphate dehydrogenase (G6PD, type B) have been raised in rabbits and used for the characterization of genetic variants of this enzyme protein. Use of the anti-G6PD antisera for estimating the specific activity of G6PD variants has recently been criticized and shown to be inadequate to this purpose. Moreover, both the specificity and strength of conventional antisera are not satisfactory enough to allow a precise characterization of G6PD variants associated with severe deficiency of activity. In an attempts to overcome thes limitations, we used cell fusion techniques to obtain anti-G6PD antibody-secreting hybridomas. By this approach, a number of antibodies directed to human G6PD were isolated after fusion of an 8-azaguanine-resistant mouse myeloma line with splenocytes from a mouse hyperimmunized with G6PD
Simple synthesis of P1P2-Diadenosine 5'-pyrophosphate
No full textPyrophosphate-linked coenzymes play essential roles in several biochemical systems. Symmetrical diadenosine-5'-pyrophosphate (Ap2A) has been synthesized from adenosine-5'-phosphate in virtually quantitative yield. The simple procedure is carried out in anhydrous pyridine using adenosine phosphoromorpholidate and adenosine monophosphate bis-(tri-n-butylammonium salt) as coupling reagents
NUTRIENT FACTORS PRESENT IN MEDIUM CONDITIONED BY A HUMAN CLONED MYELOMONOCYTIC CELL-LINE GROWTH AT LIMITING DILUTION
No full textSynthesis, uptake and intracellular metabolism of a hydrophobic tetrapeptide-peptide nucleic acid (PNA)-biotin molecule
No full text- …
