1,721,000 research outputs found
Influence of a GMO-containing diet on pancreatic acinar cells of adult mice: effects of a short-term diet reversion
No full textCirculating immune complexes in human breast cyst fluids: relationship with intracystic immunoglobulin and electrolyte levels
No full textHistochemical and morpho-metrical study of mouse intestine epithelium after a long term diet containing genetically modified soybean
No full textDiet can influence the structural characteristics
of both small and large intestine. In this
study, we investigated the duodenum and
colon of mice fed on genetically modified (GM)
soybean during their whole life span (1-24
months) by focusing our attention on the histological
and ultrastructural characteristics of
the epithelium, the histochemical pattern of
goblet cell mucins, and the growth profile of
the coliform population. Our results demonstrate
that controls and GM-soybean fed mice
are similarly affected by ageing. Moreover, the
GM soybean-containing diet does not induce
structural alterations in duodenal and colonic
epithelium or in coliform population, even
after a long term intake. On the other hand,
the histochemical approach revealed significant
diet-related changes in mucin amounts in
the duodenum. In particular, the percentage of
villous area occupied by acidic and sulphomucin
granules decreased from controls to
GM-fed animals, whereas neutral mucins did
not change
Oxidative stress and apoptosis induction in human thyroid carcinoma cells exposed to the essential oil from Pistacia lentiscus aerial parts
Full text linkBackground. Essential oils from the aerial parts (leaves, twigs and berries) of Pistacia lentiscus (PLEO) have been well characterized for their antibacterial and anti-inflammatory properties; however, poor information exists on their potential anticancer activity. Methods. Increasing concentrations of PLEO (0.01–0.1% v/v, 80–800 μg/ml) were administered to a wide variety of cultured cancer cells from breast, cervix, colon, liver, lung, prostate, and thyroid carcinomas. Fibroblasts were also included as healthy control cells. Cell viability was monitored by WST-8 assay up to 72 hours after PLEO administration. The intracellular formation of reactive oxygen species (ROS), the induction of apoptosis, and the enhancement of chemotherapeutic drug cytotoxicity by PLEO were further investigated in the most responsive cancer cell line. Results. A dose-dependent reduction of tumor cell viability was observed upon PLEO exposure; while no cytotoxic effect was revealed in healthy fibroblasts. FTC-133 thyroid cancer cells were found to be the most sensitive cells to PLEO treatment; accordingly, an intracellular accumulation of ROS and an activation of both the extrinsic and intrinsic apoptotic pathways were evidenced in FTC-133 cells after PLEO administration. Furthermore, the cytotoxic effect of the antineoplastic drugs cisplatin, 5-fluorouracil and etoposide was enhanced in PLEO-exposed FTC-133 cells. Conclusion. Taking into account its mode of action, PLEO might be considered as a promising source of natural antitumor agents which might have therapeutic potential in integrated oncology
BCR-ABL oligodeoxynucleotides modify the nucleolar structure in K562 cells but do not induce apoptosis.
No full textAnalisi ultrastrutturale dei nuclei di epatociti di topo nutriti con soia e mais geneticamente modificati
No full textBCR-ABL oligodeoxynucleotides suppress the growth and modify the nucleolar structure in K562 cells.
No full textConvegno: Programmed Cell Deat
Selective monoamine oxidase B inhibition by an Aphanizomenon flos-aquae extract and by its constitutive active principles phycocyanin and mycosporine-like amino acids
No full textAphanizomenon flos-aquae (AFA) is a fresh water unicellular blue-green alga that has been traditionally used for over 25 years for its health-enhancing properties. Recent studies have shown the ability of a proprietary AFA extract (Klamin(®)) to improve mood, counteract anxiety, and enhance attention and learning. Aim of this study was to test the monoamine oxidase (MAO) inhibition activity of the same AFA extract and of its constituents phycocyanin (AFA-PC) and mycosporine-like aminoacids (AFA-MAAs). All compounds showed a dose-dependent selective inhibition of MAO-B activity as compared to MAO-A. The IC50 values of the AFA extract (concentration 10 mg/ml), AFA-PC and AFA-MAAs were 6.4 μl/ml, 1.33 μM and 1.98 μM, respectively, evidencing a mixed-type of inhibition for the AFA extract (Ki 0.99 μl/ml), a non-competitive inhibition for AFA-PC (Ki 1.06 μM) and a competitive inhibition for AFA-MAAs (Ki 0.585 μM). These results are important to explain the neuromodulating properties of the AFA extract Klamin(®), which is rich in phenylethylamine, a general neuromodulator, that would nevertheless rapidly destroyed by MAO-B enzymes without the inhibitory activity of the synergic active principles AFA-PC and AFA-MAAs. The present investigation thus proposes the extract as potentially relevant in clinical areas such as mood disorders and neurodegenerative diseases
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