1,721,198 research outputs found
Caratterizzazione di elementi genetici naturali e costruzione di vettori artificiali per lo studio della replicazione del DNA e per l’espressione eterologa in Sulfolobus solfataricus
No full textIDENTIFICAZIONE E SOVRAESPRESSIONE DI GENI DA TERMOFILI COINVOLTI NELLA MODIFICA E ATTIVAZIONE DI GLICOSIDI
No full textANALISI DEI MECCANISMI MOLECOLARI DI DETOSSIFICAZIONE DA ALDEIDI AROMATICHE IN S. SOLFATARICUS PER LO SVILUPPO DI BIOSENSORI BATTERICI
No full textCARATTERIZZAZIONE DI ELEMENTI NATURALI E SVILUPPO DI VETTORI DI CLONAGGIO ED ESPRESSIONE PER LARCHAEON SULFOLOBUS SOLFATARICUS
No full textENGINEERING OF GLYCOSIDE HYDROLASES OF BIOTECHNOLOGICAL INTEREST FROM EXTREMOPHILIC ORGANISMS
No full textGENETIC AND MOLECULAR CHARACTERIZATION OF THE VIRUS/PLASMID HYBRID PSSVX ISOLATED FROM S.ISLANDICUS REY 15/4
No full textMonovalent cations requirement of the fructose 1,6-bisphosphate-activated pyruvate kinase from E. coli.
No full textThe fructose 1,6-bisphosphate-activated pyruvate kinase from Escherichia coli has been purified by a simplified procedure, which gives a homogeneous enzyme in approximately half the working time required by other methods and is suitable for large scale preparations. The activity of the enzyme is strictly dependent on the presence of monovalent cations. Enzyme activity is elicited by K+ and NH4+, but not by Na+. Homotropic cooperativity is displayed in the activation by K+ and NH4+ and heterotropic effects are reciprocally exerted by monovalent cations and other ligands, such as phosphoenolpyruvate and fructose 1,6-bisphosphate. The allosteric nature of such interactions is suggested by changes in heat stability of the enzyme induced by K+ and fructose 1,6-bisphosphate. NH4+, but not K+, at high concentrations, cause an inhibition of enzyme activity
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