1,720,982 research outputs found
St John's wort modulation and developmental expression of multidrug transporters in the rat.
No full textThiopurine-S-methyltransferase genotype and the response to azathioprine in inflammatory bowel disease.
No full textGlucocorticoid resistance in a girl with Takayasu’s arteritis
No full textA case of a 16 year old girl with Takayasu’s arteritis is described; the girl was treated with oral prednisone and methotrexate, with a very poor clinical response and, after 1 year’s treatment with high dose steroids, no sign of exogenous Cushing’s syndrome was evident. The whole cell [3H]dexamethasone binding assay revealed a reduced number of receptors per cell in the patient’s PBMCs in comparison with ten patients, treated with GCs and responsive to treatment, and six healthy controls. Binding affinity (Kd) was, on the contrary, not modified. No mutation in the glucocorticoid receptor gene was identified, however an increased expression of hsp90 mRNA in the patient’s PBMCs was evident, which could give a molecular explanation for the GC resistance
Inhibitors of adriamycin-induced histamine release in vitro limit adriamycin cardiotoxicity in vivo.
No full text
ABCB1 gene polymorphisms and expression of P-glycoprotein and long term prognosis in colorectal cancer
No full textBACKGROUND: P-glycoprotein (Pgp), encoded by the ATP-binding cassette B1 (ABCB1) gene, is an efflux transporter located on the luminal side of intestinal epithelial cells, which protects the gut from endogenous and exogenous toxins. The association of two ABCB1 polymorphisms with the occurrence of colon cancer and long-term prognosis was evaluated in a selected patient population. The expression of Pgp in neoplastic and normal intestinal mucosa was also studied. PATIENTS AND METHODS: Archival material from 51 patients, in Dukes stage B2 or C, treated for 6 months with 5-fluorouracil plus leucovorin was retrieved. The G2677T and C3435T polymorphisms were studied and immunohistochemical analysis of the tumor and adjacent normal tissue was performed. RESULTS: The distribution of wild-type and polymorphic genotypes was similar in the patients and controls and in the patients who relapsed and those who remained event-free for 5 years. Cox proportional hazard model indicated an increased probability of relapse for older patients (p = 0.042) and C stage tumors (p = 0.030). Pgp expression was significantly lower in cancer tissue compared to normal mucosa (p < 0.001) and was related to grading, being lower in poorly-differentiated tumors (p < 0.05); however, no relationship was seen between Pgp expression, genotype and long-term prognosis. CONCLUSION: G2677T and C3435T polymorphisms are not associated with colon cancer risk and prognosis in a selected patient population
Interruption of mesalamine and reduction of the blood concentration of the active metabolites of azathioprine: possible causes of ulcerative colitis relapse.
No full textRole of MDR1 gene polymorphisms in gingival overgrowth induced by cyclosporine in transplant patients.
No full textBACKGROUND AND OBJECTIVE:
The aim of the present study was to determine the association between genotypes of the MDR1 gene, encoding P-glycoprotein, and gingival overgrowth in transplant patients treated with cyclosporine, and to evaluate the effect of periodontal treatment in these patients.
MATERIAL AND METHODS:
Fifty transplant patients receiving therapy with cyclosporine and suffering from gingival overgrowth were subjected to nonsurgical periodontal treatment and received oral hygiene instructions. Hyperplastic index, periodontal probing depths, bleeding and plaque scores were recorded at baseline and after 3 and 6 mo. Patients were dichotomized into two groups: those with a hyperplastic index of or = 30% (clinically significant gingival overgrowth). MDR1 C3435T and G2677T polymorphisms were evaluated in all patients and in 100 controls.
RESULTS:
At baseline, 32 patients (64%) had minimal gingival overgrowth and 18 patients (36%) had clinically significant gingival overgrowth. The mutated C3435T genotype was significantly more frequent in the second group (p < 0.019). The significant association between gingival overgrowth and the 3435TT genotype was confirmed by logistic regression analysis (p < 0.031). The differences in hyperplastic index, observed at baseline between patients with the TT genotype and those with the CC/CT genotype disappeared in the second and third evaluation. The mean monthly change of the square root of the gingival overgrowth scores for all patients, assessed using linear models, was significantly different from baseline (-0.17 points per month, p < 0.00001); and this was particularly evident in subjects with renal transplant (-1.62, p < 0.01).
CONCLUSION:
Aetiological periodontal and self-performed maintenance therapy is effective in reducing gingival overgrowth, particularly in subjects with the 3435TT genotype and in patients with renal transplant
Biochemical and microscopic evidence for the internalization of drug-containing mast cell granules by macrophages and smooth muscle cells
No full text- …
