1,721,008 research outputs found
The pregnancy diagnosis in buffalo species: laboratory methods
No full textPregnancy diagnosis plays an important role in the reproduction management of ruminants since embryonic
mortality has a substantial impact on the fertility of a herd. Most of the embryonic losses occur during the first days after
fertilization and during the process of implantation. So it is very important to discriminate, with an early pregnancy
diagnosis, non-pregnant from pregnant animals. Hormone analysis to detect pregnancy may be utilize as a more simple
technique as an alternative of rectal palpation or ultrasound. In the last years, a large polymorphic family of placentaexpressed
proteins has been discovered in ruminant species and used for pregnancy diagnosis. Members of this family
are named pregnancy-associated glycoproteins (PAG), being synthesized in the mono-and binucleate cells of the
ruminant’s trophectoderm. Part of them is released in the maternal blood circulation where they can be assayed by
different laboratory techniques. Due to large variety of expressed molecules and to large variations in the posttranslational
processing of the PAG, different immuno-systems present different ability to quantify the PAG released in
blood. The assay of PAG can also bring very interesting information for researchers working in programs focused on the
study of embryonic and fetal mortalities, as well as on embryo biotechnology, animal nutrition or infections diseases
resulting in pathologies affecting the pregnancy
Produzione di embrioni in vitro nella specie bovina - Analisi di differenti condizioni di coltura
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Timing of the LH peak and P4 blood concentration after the ovulations in buffalo cows during different superovulatory treatments.
No full textAssessment of different functional parameters of frozen-thawed buffalo spermatozoa by using cytofluorimetric determinations
No full textFlow cytometry is a useful tool that provides an accurate, objective and rapid evaluation of semen quality. The use of this technique could significantly improve the quality of buffalo semen samples used in artificial insemination. This study was carried out to evaluate, by flow cytometry, frozen-thawed buffalo spermatozoa quality parameters such as: sperm viability by SYBR-14/propidium iodide staining; mitochondrial function by JC-1 potentiometric probe; sperm chromatin stability (SCSA) by acridine orange and acrosome reaction by FITC-PNA staining. Semen samples from 5 Italian Mediterranean buffalo bulls were used. Sperm viability was not different between bulls and ranged from 33.4 to 43.6%. A consistent rate (55.1±10.8%) of sperm cells showed high mitochondrial membrane potential (Δψhigh), with no significant differences between subjects. SCSA differed significantly between the 5 buffalo bulls, moreover data showed high stability within each buffalo. DNA fragmentation indexes (DFI), such as %-DFI, -DFI, SD-DFI, were 11.2±8.6, 153.3±24.6 and 81.6±21.2, respectively. Regarding acrosome reaction (AR), the percentage of acrosome-reacted live (ARL) and acrosome-reacted dead (ARD) spermatozoa was 0.3±0.2 and 15.3±5.5 respectively. This functional parameter differed significantly between buffalo bulls and showed high stability. Following to Ca2+ ionophore A23187 for 3 hrs, AR significantly differed between subjects and was characterized by an increase of both ARL (10.8%) and ARD population (22.0%). The present study indicates that flow cytometry could be a useful tool for a quick multiparametric evaluation of sperm quality in buffalo. In particular, SCSA and AR resulted sperm functional parameters sensitive enough for the diagnosis of frozen-thawed semen fertilizing potential
Use of GnRH Treatment Based on Pregnancy-Associated Glyco-Proteins (PAGs) Levels as a Strategy for the Maintenance of Pregnancy in Buffalo Cows: A Field Study
Get PDFThe aim of the study was to evaluate the effects of GnRH administrated at day 35 after artificial insemination (AI) on the reproductive performance of buffalo cows. In ten buffalo farms in the period January–February, 481 buffalo cows were subjected to estrus synchronization protocol and fixed-time artificial insemination (Ovsynch–TAI program). Radioimmunoassays (RIA) for pregnancy-associated glycoproteins (PAGs) were used to detect pregnancy at day 28 after AI (cut-off value: ≥1 ng/mL). Among pregnant subjects, those with PAG values between 1 and 2.5 ng/mL were considered at risk of embryonic mortality (EM) and were assigned into two groups: treated (T; n = 57) control (C; n = 57). Treated buffaloes received 0.01 mg of buserelin acetate intramuscularly on day 35 after AI, whereas control buffaloes received no treatment. The pregnancy diagnosis was confirmed at day 60 through PAGs level and rectal palpation. The treatment with GnRH had a significant effect (p < 0.001) in reducing EM. Between days 28 and 60 after AI, the animals that experienced EM were only 2/57 in the T group, while were 13/57 in the C group. Moreover, GnRH treatment produced a significant increase (p < 0.001) in the PAG concentration between day 28 and day 60. Administration of GnRH at day 35 after AI in animals considered at risk of low embryo survival based on PAG levels allowed a reduction in pregnancy losses and improved the pregnancy rate during low-breeding season in buffalo
Age prediction obtained by ultrasonographic fetal measurement in the buffalo.
No full textUltrasonography has been reported as the method of choice for early pregnancy diagnosis. The aim of this study was to evaluate the mesaurements of some specific somatic parameters in the foetus, such as crown rump length (CRL), biparietal diameter of the cranium (BPD), abdominal diameter (AD), muzzle occipital length (MOL) and femur, tibia and metatarsial bone lengths in order to estimate foetal growth. Several regression equations have been formulated in order to do this in buffalo species
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