1,721,063 research outputs found
The pregnancy diagnosis in buffalo species: laboratory methods
No full textPregnancy diagnosis plays an important role in the reproduction management of ruminants since embryonic
mortality has a substantial impact on the fertility of a herd. Most of the embryonic losses occur during the first days after
fertilization and during the process of implantation. So it is very important to discriminate, with an early pregnancy
diagnosis, non-pregnant from pregnant animals. Hormone analysis to detect pregnancy may be utilize as a more simple
technique as an alternative of rectal palpation or ultrasound. In the last years, a large polymorphic family of placentaexpressed
proteins has been discovered in ruminant species and used for pregnancy diagnosis. Members of this family
are named pregnancy-associated glycoproteins (PAG), being synthesized in the mono-and binucleate cells of the
ruminant’s trophectoderm. Part of them is released in the maternal blood circulation where they can be assayed by
different laboratory techniques. Due to large variety of expressed molecules and to large variations in the posttranslational
processing of the PAG, different immuno-systems present different ability to quantify the PAG released in
blood. The assay of PAG can also bring very interesting information for researchers working in programs focused on the
study of embryonic and fetal mortalities, as well as on embryo biotechnology, animal nutrition or infections diseases
resulting in pathologies affecting the pregnancy
Pre-ovulatory Follicle size, LH peak Values and Pregnancy induced by three synchronization treatment in buffalo cows during non breeding season
No full textLactating buffalo cows (n=18) were assigned to 3 different synchronization treatments: group A (n=7) treated with PRID for 10 days + 1000 IU PMSG and PGF2α (0.15mg cloprostenol) on day 7; group B (n=6) treated as group A but PMSG and PGF2α were administered on day 10; group C (n=5), treated with GnRH; (150μg gonadorelin) on day 0 + PGF2α on day 7 + GnRH on day 9 (Ovsynch protocol). Buffaloes were artificially inseminated two times (at 72 and 96h from PRID removal) in group A and B and once (at 16h from the second GnRH injection) in group C. The size of pre-ovulatory follicle was assessed by ultrasonography every 12h, starting 24h from PRID removal (group A and B) or 12h from PGF2α injection (group C); at the same time blood samples were collected every 4h for the measurement of plasma LH. The pre-ovulatory follicle size was larger (P<0.05) in group A as compared to B and C groups and in ovulating as compared to non-ovulating animals (P<0.001); in animals that became pregnant the differences tended to be significant (P<0.08) as compared to non-pregnant ones. LH peak values were higher (P< 0.05) in group A as compared to B and C groups and in ovulating as compared to non-ovulating animals (P<0.001). The ovulation rate (OR) was influenced by treatments: in group A it was 100%; in group B and C the ORs were 50% and 60%, respectively. The conception rates were 71, 4%, 16, 7% and 40% in group A, B and C, respectively. In conclusion the ovulation and conceptions rates were higher in animals of group A; that showed also increased pre-ovulatory follicle size and LH peak values
Use of PSPB ELISA for the detection of pregnancy in cattle and buffaloes - Congrès mondial de buiatrie
No full textProgesterone Plus PMSG Priming in seasonally Anovulatory Lactating sarda ewes Exposed to the Ram Effect
No full textThe aim of this trial was to evaluate the effectiveness (fertility and lambing) of priming with a single injection of progesterone plus PMSG in anovulatory lactating Sarda ewes subjected to the ram effect (RE) in spring. Thirty ewes (P4 group) were i.m. injected with 30 mg progesterone and 500 IU PMSG 36 h before ram introduction (d 0). This treatment was compared to a 12-day treatment with fluorogestone acetate intravaginal sponges that was followed by injections of 350 IU PMSG upon sponge withdrawal (FGA group, n=30). All ewes responded to RE, showing plasma progestrone concentrations >1 ng/mL between d 6 and 12 (FGA) or 6 and 9 (P4). Eighty-nine percent of the P4 ewes conceived at first ovulation, and 11% conceived following a short estrus cycle. Lambings occurred on d 150.4 +/- 3.9, and the lambing rate was 100%. The fertility of the FGA ewes was 83% for the induced ovulation and was 7% for the second ovulation after a normal cycle. The FGA ewes lambed on d 149.8 +/- 4.4, and the lambing rate was 83%. Two abortions were recorded for the FGA ewes, which had higher prolificacy than the P4 group (2.2 +/- 0.8 vs. 1.8 +/- 0.4, respectively; P<0.05). Both fertility and the lambing rate were high in both groups, with a high degree of estrus synchronization, and there were no significant differences between the groups. We concluded that priming of lactating Sarda ewes in spring with P4+PMSG before RE is an effective and competitive method (cheaper and more practical than FGA+PMSG) of inducing fertile ovulations in these ewes
Variazioni della concentrazione ematica di cortisolo e beta-endorfine in pecore e agnelle in corrispondenza della monta
No full textDietary supplementation of Yerba Mate (Ilex paraguariensis) during the dry period improves redox balance in lactating dairy cows
Get PDFThirty-six pregnant Holstein–Friesian cows were used to study the effect of Yerba Mate (YM) supplementation during the dry period on redox balance. The treatments groups were Control (no YM), YM 250 (250 g/cow/day), and YM 500 (500 g/cow/day). Blood samples were obtained 30 days prepartum, at calving, and monthly postpartum until four months post calving. Liveweight (LW) and body condition score (BCS) were assessed prepartum, at calving, and then postpartum monthly until the end of the trial. Plasma was analyzed for hydroperoxides (d-ROMs), advanced oxidation protein products (AOPP), and biological antioxidant potential (BAP). The oxidative stress index (OSI) was calculated as OSI = ROMs/BAP 100. Cows were milked twice daily, and milk yield data were recorded daily. Redox balance was improved by YM supplementation, as reflected in the lower OSI values observed in the YM groups. Yerba Mate supplementation significantly affected LW, but did not affect BCS. Milk yield averaged 28.1 0.40, 29.0 0.48, and 29.9 0.46 L/cow/day in the Control, YM 250, and YM 500 groups, respectively, but was not significant. Nutritional manipulation during the dry period with Yerba Mate has demonstrated the potential to improve redox balance and milk yiel
Relationship between oxidative stress and success of artificial insemination dairy cows in a pasture-based system
No full textThis study was designed to evaluate whether the outcome of artificial insemination (AI) was affected by the metabolic and oxidative status of dairy cows. Seventy-nine inseminations in 40 cows, were classified, on the basis of blood progesterone (P4) and pregnancy-associated glycoprotein (PAG) concentrations and clinical confirmation of pregnancy into, three categories: (1) positive (AI+, resulted in pregnancy, n=26; 33%), (2) negative (AI-, did not result in pregnancy, n=49; 62%), and (3) embryonic mortality (EM, n=4; 5%). Reactive oxygen metabolites, biological antioxidant potential, oxidative stress index, body condition score, glucose, total proteins, albumin, urea, non-esterified fatty acids (NEFAs), cholesterol, triglycerides, haptoglobin and advanced oxidative protein products (AOPPs) were measured on the day of AI (day 0), and 30 and 42days later. Cows with EM had lower BCS scores (2.5) than AI+ (2.8) and AI- (2.9) cows (P<0.05). During the post-partum period, body condition score (BCS) increased and NEFAs decreased (P<0.05) suggesting a recovery from the negative energy balance (NEB). The only significant differences found were that the mean concentration of AOPPs was higher and that of albumin lower in EM cows than in AI+ and AI- (P<0.05) animals. Plasma concentration of reactive oxygen metabolites and biological antioxidant potential were not related to AI outcome. Further studies are required to confirm this finding and to clarify the role of oxidative status on cows' fertility
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