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GLIOBLASTOMA IN A PATIENT WITH PROFESSIONAL EXPOSURE TO THE ONCOGENIC POLYOMAVIRUS SV40.
No full textAIM. In previous investigations transforming sequences of the DNA tumor virus, named simian virus 40 (SV40), have been found in human brain tumors, thereby suggesting a role for SV40 in human tumorigenesis. A recent study reported the case of a meningioma in a SV40-exposed scientist. The aim of this study was to investigate the presence of specific SV40 sequences in a laboratory investigator, professionally exposed since 1998 to the oncogenic SV40, who developed a glioblastoma multiforme.
METHODS. DNA from tumor and blood specimens was analyzed by PCR and filter hybridization. RESULTS. Tumor and blood samples from the patient tested negative for SV40 sequences.
CONCLUSION. Our data do not support in this clinical case the link between the SV40 exposure and the brain tumor
Gram-negative bacterial lipopolysaccharide retention by a positively charged new-generation filter.
No full textRemoving endotoxins is an important target in the pharmaceutical industry and in clinical practice. A filter
introduced into an intravenous line prevents microbiological contamination, but to date no filters have
retained bacterial endotoxins. In our study, we assayed a new-generation filter which is able to capture
endotoxins from solutions
Cellule mesoteliali umane trasformate da SV40 come modello di studio del mesotelioma maligno della pleura
No full textASPETTI GENETICI DELLE PATOLOGIE DEL COLON
No full textIl cancro del colon è una malattia genetica delle cellule somatiche di questo distretto anatomico. Il 5% della popolazione, senza distinzione tra i due sessi, sviluppa il cancro colorettale. L’elevata prevalenza di questa patologia la fa annoverare tra gli aspetti sanitari importanti per la popolazione. Il cancro del colon può essere sporadico, ereditario o famigliare (Tabella 1). La forma sporadica, con o senza predisposizione famigliare, comprende circa al 70% dei casi e insorge principalmente in persone con una età maggiore di 50 anni. Le alterazioni del DNA delle cellule somatiche sono dovute a diversi fattori, come i carcinogeni ambientali assunti con la dieta, e a mutazioni geniche e/o epigenetiche che si osservano principalmente con l’invecchiamento.
Meno del 10% del cancro al colon, come per altri tumori umani, è di origine ereditaria. I casi ereditari si manifestano sia in assenza che in presenza di poliposi coliche. I casi con poliposi sono suddivisi in poliposi adenomatosa famigliare e amartomatosi. I non poliposici predominanti comprendono il cancro del colon ereditario non poliposico (HNPCC), o sindrome di Lynch I, e la sindrome famigliare da cancro o sindrome di Lynch II. La sindrome di tipo I interessa esclusivamente il colon, mentre nel tipo II l'interessamento del colon è sempre predominante ma in associazione ad altre neoplasie come il carcinoma dell'utero, delle ovaie, del tratto epatobiliare, del pancreas, delle vie urinarie. Queste sindromi sebbene poco comuni forniscono importanti spiegazioni sulla biologia di tutti i tipi di cancro colorettale.
Il terzo tipo di cancro al colon è conosciuto come cancro del colon famigliare. Nelle famiglie affette, il cancro del colon si sviluppa troppo frequentemente per essere considerato un cancro sporadico, ma tuttavia gli osservati sono meno degli attesi rispetto alla forma sindromica ereditaria. Fino ad un 25% di tutti i casi di cancro al colon dovrebbe rientrare in questa categoria
I lipopolisaccaridi batterici come complicanza nell’impiego della terapia endovenosa
No full textPOSTE
Sequenze specifiche del DNA del virus oncogeno SV40 in buffy coats umani.
No full textCOMUNICAZIONE ORALE
Innovative methods for the detection of the oncogenic virus SV40 in human samples
No full textSimian virus 40 (SV40) is a potent DNA oncogenic polyomavirus, which seems to be also a human virus. Molecular biology and immunologic studies indicate that SV40 may be contagiously transmitted in humans by horizontal infection. Its spread in humans may occur by different ways, such as sexual, urine, haematogeneous, respiratory and orofecal.routes. SV40 is considered a co-factor in the onset/progression of specific human tumours. Indeed, SV40 DNA sequences have been found in human brain and bone tumors, mesotheliomas and lymphomas. Moreover, SV40 sequences and the expression of its oncoprotein, the large T antigen (Tag), were detected in blood and sperm specimens of neoplastic patients and normal individuals. SV40 infectious virions were found in urine and stool samples, suggesting that SV40 is also an environmental infectious agent. Recent studies show SV40 footprints in specimens from healthy children and adults. These data suggest that the oncogenic virus may be contagiously transmitted within the family. Altogether these results indicate that SV40 can be considered an emerging human viral agent and a putative new pathogenic virus. We believe that the presence of SV40 should be investigated in the human population, as well as in foods, beverages and in the environment. Our laboratory developed new molecular biology and immunologic assays, employing PCR and ELISA techniques. By PCR techniques, both qualitative and quantitative, we detected with a different prevalence SV40 DNA sequences in human tumors, and tissues from healthy individuals. The ELISA test, employing synthetic peptides corresponding to specific SV40 epitopes of the capsid and Tag proteins, was used to carry out epidemiologic and screening tests to verify the presence of SV40 antibodies in sera from oncologic patients and normal blood donors
Simian virus 40 in humans
No full textAbstract Simian virus 40 (SV40) is a monkey virus that was administered to human populations by contaminated vaccines which were produced in SV40 naturally infected monkey cells. Recent molecular biology and epidemiological studies suggest that SV40 may be contagiously transmitted in humans by horizontal infection, independently from the earlier administration of SV40-contaminated vaccines. SV40 footprints in humans have been found associated at high prevalence with specific tumor types such as brain and bone tumors, mesotheliomas and lymphomas and with kidney diseases, and at lower prevalence in blood samples from healthy donors. Contrasting reports appeared in the literature on the circulation of SV40 in humans by contagious transmission and its association, as a possible etiologic cofactor, with specific human tumors. As a consequence of the conflicting results, a considerable debate has developed in the scientific community. In the present review we consider the main results obtained by different groups investigating SV40 sequences in human tumors and in blood specimens, the putative role of SV40 in the onset/progression of specific human tumors, and comment on the hypotheses arising from these data.</p
Bacterial Lipopolysaccharide Retention by a Positively Charged Filter. Letter to the Editor, Authors' Reply
No full textWe find reasonable most of the comments and suggestions
from Dr. Ortolano and colleagues concerning the article by
Bononi et al. (1). In reply to the first comment, we wish to
point out that the reduced number of words, no more than
50, published in the abstract of the short-form paper did not
allow us to be completely specific in the sentence “A filter
introduced into an intravenous line prevents microbiological
contamination, but to date no filters have retained bacterial
endotoxins,” which should be followed by “with an efficiency
of 100%.” Indeed, in our assays, both Pall and GVS positively
charged filters were able to retain bacterial lipopolysaccharide.
However, the analysis carried out by the Limulus
amoebocyte lysate test does not allow the challenge of positively
charged filters for their absolute efficiency in retaining
the bacterial lipopolysaccharide (1). The second comment
is correct. Nonetheless, in our experience, GVS positively
charged filters performed well in clinical settings. No complaints
came to our attention from different hospitals which
were GVS customers (our unpublished data). We agree with
the final suggestion to carry out additional experiments with
“more clinically relevant salt-containing intravenous solutions.”
This analysis is feasible, and it will be part of our next
study
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