4,736 research outputs found
SV2A/B KO neurons are resistant to TeNT.
No full text<p>(A) Mouse spinal cord neurons with the following genotypes: WT, SV2B KO [SV2A (+/+) SV2B (−/−)], and SV2A/B KO [SV2A (−/−) SV2B (−/−)], were exposed to HCR/T (50 nM). HCR/T fluorescence in SV2A/B KO neurons was dramatically reduced as compared to WT and SV2B KO. (B) Quantification of HCR/T binding: fluorescence was reduced by 30% and 50% for SV2B KO and SV2A/B KO neurons, respectively. Error bars represent SD, WT n = 9, SV2B KO n = 11, SV2A/B KO n = 12, ***p≤0.001. (C) SV2B KO and SV2A/B KO cultures were exposed to TeNT (20 nM) and BoNT/A (10 nM). Cell lysates were subjected to immunoblot analysis and probed for syb II, SV2, SNAP-25, and actin. Syb II in SV2A/B KO neurons was largely protected from TeNT action until resensitized through lentiviral expression of SV2A or SV2B; arrow indicates the BoNT/A cleaved form of SNAP-25. (D) SV2B KO and SV2A/B KO spinal cord neurons from were assayed for susceptibility to TeNT. Syb II was cleaved by 5 nM TeNT in SV2B KO neurons, while syb II was protected from TeNT in SV2A/B KO neurons. SV2A/B KO neurons could be resensitized to TeNT, upon lentiviral expression of SV2A. (E) Three putative glycosylation sites in SV2A were removed by creating N to Q mutants (residues 498, 548 and 573) and were expressed in SV2A/B KO neurons along with WT SV2A. Syb II was cleaved by TeNT in neurons reinfected with WT SV2A as well as the three mutants. (F) WT and SV2B KO mice were injected with the indicated amounts of TeNT and their time-to-death was recorded. SV2B KO mice were more than five-times more resistant to TeNT as compared to their WT counterparts. (G) WT and SV2A/B KO neurons were cultured and treated with BoNT/F at the indicated concentrations. Cell lysates were probed for syb II and syp by immunoblot analysis. WT and SV2A/B KO neurons exhibited similar sensitivities to BoNT/F.</p
Wright Model B Flyer tail protruding from a tent
No full textA Wright Model B Flyer in a tent near the Wright Bros. sign at Belmont Park. The tail of the Model B is resting on a sawhorse outside the tent. This photograph is attributed to the U.S. Army Air Corps, 2nd Photo Section, Langley Field, Virginia.https://corescholar.libraries.wright.edu/special_ms1_photographs/2391/thumbnail.jp
An exposure-free tool for monitoring adult malaria mosquito populations.
No full textCatches of Anopheles gambiae and An. arabiensis with the Ifakara Tent Trap-model B (ITT-B) correlate better with human landing catches than any other method but fail to reduce the proportion of blood-fed mosquito caught, which indicates that users are exposed to bites during collection. An improved C model (ITT-C) was developed and evaluated by comparing with ITT-B in semi-field and full-field conditions in southern Tanzania. The sensitivity of the ITT-C was approximately two times that of the ITT-B: relative rate (95% confidence interval) = 1.92 (1.52-2.42), 1.90 (1.48-2.43), and 2.30 (1.54-3.30) for field populations of An. arabiensis, Culex spp., and Mansonia spp., respectively. The ITT-C caught 73% less blood-fed An. arabiensis than the ITT-B in open field experiments and none in semi-field experiments, which confirmed that the C design is a safe trapping method. Validation of ITT-C by comparison with human landing catches and parasitologic measures of human infection status may be necessary to confirm that this design produces consistent and epidemiologically meaningful results
Photo Tent (55b26)
No full textA view of a photo tent at a 319th Bomb Group camp site. The double tent helps provide coolness. One black and white photograph
Tent Frames
No full textTwo rows of tent frames with a dirt path through the two rows. ""1850.0011. CCC Camp S-223 McCall, ID 1935 Tent frames at old camp. May have been either a CCC summer camp or National Guard Camp Author: David Lyon Print (x4) (Negative in '1850 D. Lyon Negatives') 6 A, 23A, & 24A Color"".S-22
Shower Tent
No full textPhoto of a tent on a platform above the edge of Payette Lake. Writing beneath the photo reads: ""1850.0008. CCC Camp S-223 McCall, ID 1935 Shower Tent Erected on Platform of Old Camp. Author: David Lyon Print (x2) (Negative in '1850 D. Lyon Negatives') 3A & 21 A Color"".S-22
Effects of climate and forest structure on duration of forest tent caterpillar outbreaks across central Ontario, Canada
No full textAbstract: We examined the effect of forest structure and climate enlarge-scale and long-term patterns of outbreaks of forest tent caterpillar, Malacosoma disstria Hbn., across central Ontario. This was done using previously published data on outbreak duration and forest heterogeneity, combined with high-resolution climatic data simulated by the recently developed Ontario Climate Model. Our analysis, which eliminates some of the spatially confounding effects of forest structure and climate, suggests that both the predicted long-term temperature minimum for the coldest month and the predicted growing degree-days in the first 6 weeks of the growing season are important determinants of outbreak duration, with colder weather being associated with shorter outbreaks. Forest heterogeneity accounts for more variation in outbreak duration than either of the climatic variables
Interpolation and Embeddings of Weighted Tent Spaces
No full textInternational audienceGiven a metric measure space X, we consider a scale of function spaces T^{p,q}_{s}(X), called the weighted tent space scale. This is an extension of the tent space scale of Coifman, Meyer, and Stein. Under various geometric assumptions on X we identify some associated interpolation spaces, in particular certain real interpolation spaces. These are identified with a new scale of function spaces, which we call Z-spaces, that have recently appeared in the work of Barton and Mayboroda on elliptic boundary value problems with boundary data in Besov spaces. We also prove Hardy–Littlewood–Sobolev-type embeddings between weighted tent spaces
Arab Tent A and B
No full textQuilt pattern from the Linda Lowe Quilt Patterns Collection titled Arab Tent A and B.https://scholarworks.moreheadstate.edu/lowe_quilt_patterns/1035/thumbnail.jp
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