140 research outputs found
Hanen og raeven fra The Canterbury Tales
No full textHere is the Danish version of Chanticleer and the Fox published by Thomas Y. Crowell in 1958. This version features the same cover picture showing the main characters, including both the rooster and the fox. It even uses the same design for its endpapers. As I wrote there, this is an enjoyable large-format book for children. The whole argument about dreams is dropped from the original. The best illustrations are those introducing Chanticleer and the fox (about 12 and 20, respectively). How nice to meet an old friend in a new place! A web reference seems to confirm that it was published in 1958.This is a hardbound book (hard cover)Language note: Danishaf Geoffrey Chaucer. Oversat af Cecil Bødke
Figure 4. Pulmonary gene expression profiles of genes related to T-cells, B-cells, MHC-I and II and novel genes in protected and unprotected mice following B. pertussis challenge.
No full textExpression profiles of genes in the lungs related
to (A) T-cells, (B) B-cells, (C) and antigen presentation by MHC-I and MHC-II
were selected according to GO-BP terms, KEGG pathways and text mining. In
addition, genes with unknown or poorly understood function that showed
interesting gene expression profiles in protected mice were listed as (D) novel
genes. Additionally, the color codes of the six clusters from Figure 2 are added
Figure 5. Cytokine profiles in the lungs following B. pertussis challenge in unprotected and protected mice.
No full textPulmonary concentrations of (A) CCL4, (B) CCL11,
(C) VEGF, (D) G-CSF, (E) CXCL1, (F) CXCL2, (G) CXCL5, (H) CXCL9, (I) CXCL10,
(J) IL-17A, (K) IL-5, (L) TNFα and (M) M-CSF were analyzed before and after a B. pertussis challenge in unprotected (lighter
blue bars) and protected (dark blue and red bars) mice, as indicated. Data
represented as mean concentrations of individual values (n = 3). Significant values were calculated by
one-way ANOVA with multiple comparison compared to the pre-challenge level (D0)
of unprotected mice or protected mice (* = pp<0.01,
and *** = pp<0.0001)
Figure 7. Serum and pulmonary antibody profiles in unprotected and protected mice following B. pertussis challenge.
No full textSerum IgA, IgG, and IgG subclass responses specific
for (A) OMV, (B) Ptx, (C) Prn, (D) FHA, and (E) Fim2/3 were determined by using
a MIA. Data were obtained in naive mice and protected mice prior to challenge (D0)
and 14 days post infection (p.i.) or 14 days post-challenge (p.c.) (n=3/time
point). (F) Pulmonary IgA responses against these antigens were determined on
the same time points. * = p<0.05 experimental group versus unprotected group
(D0), + = p<0.05 protected group (D0) versus protected group (14 days p.c.).
(G) The kinetics of the anti-OMV IgA antibody formation in lung lysates were analyzed
at more time points (n=3/time point) and expressed in fluorescence intensity
(F.I.). **** = p<0.0001, challenged unprotected or protected group versus
unprotected or protected group (day 0), ++ and +++ = p<0.01 and p<0.001
unprotected group versus protected group (for each time point). (H) Western
blot on separated B. pertussis B1917
proteins was performed with pooled lung lysates (1:50) of unprotected and protected
mice prior to challenge (D0), and of protected mice 14 days p.c. with IR800-labeled
secondary antibody. Left panel shows whole protein range (260kDa-B. pertussis lysate. Right panel shows
more detailed separation of the 110-60kDa protein range. Antigen identification
for Vag8 and LPS is depicted
Figure 6. Serum cytokine profiles and percentage of splenic neutrophils following B. pertussis challenge in unprotected and protected mice.
No full text(A) The serum concentrations of 33 cytokines were
analyzed before and after a B. pertussis
challenge in unprotected (lighter blue bars) and protected (dark blue and red
bars) mice, as indicated. Concentrations of CXCL13, CCL11, CXCL1, G-CSF, IL-6,
CXCL10, and IL-13 serum were significantly altered and represented as mean
concentrations of individual values (n = 3). Significant values were calculated by one-way ANOVA with
multiple comparison compared to the pre-challenge level (D0) of unprotected
mice or protected mice (* = ppp<0.001).
(B) The percentage of Gr1+ cells (neutrophils) was determined over
time in the spleen of unprotected and protected mice by using Flow cytometry (*
= p
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Systems vaccinology : molecular signatures of immunity to Bordetella pertussis
No full textThe worldwide resurgence of whooping cough (pertussis), even in highly vaccinated populations, demands improved pertussis vaccines. In this thesis a systems vaccinology approach is applied to deepen knowledge of the immune responses evoked by different pertussis vaccines and compare this with a Bordetella pertussis infection since the latter induces robust protection. Infection-induced responses in mice conferred sterilizing protection that is caused by systemic immunity but more importantly by mucosal IgA, T-helper (Th)1/Th17 responses, and ‘trained’ innate immune cells in the lungs. An experimental outer membrane vesicle vaccine (omvPV) was compared with the two licensed vaccines, acellular vaccine (aPV), whole-cell vaccine (wPV) as well as a B. pertussis infection. OmvPV evoked a different immunoproteomic profile with respect to antibody levels, antigen specificity, and subclass distribution. Furthermore, omvPV confers equal protection in mice as wPV, but with a lower inflammatory response. In this thesis it is also shown that the immunization route is critical. Although subcutaneous omvPV immunization is effective, pulmonary administration lead to superior protection, comparable to infection-induced immunity and included hallmarks of protection such as pulmonary Th17 cells and mucosal IgA. The molecular and cellular signatures described in this thesis may have an important contribution to enhanced pertussis immunity
Figure 1. Design and baseline parameters of a B. pertussis challenge model in protected and naive unprotected mice.
No full text(A) Schematic diagram of animal pre-treatment,
sacrifice, sampling and systems analysis on 4 hours and 2, 7, 10 and 14 days
p.c. in a B. pertussis challenge model
in protected and unprotected BALB/c mice. Pulmonary transcriptomic profile,
percentage of splenic Gr1+ cells (neutrophils), serum and lung
cytokine profiles, serum and lung antibody profiles, and specific splenic CD4+CD44+
T-cells were assessed at the given time points in protected and unprotected mice.
(B) Study parameters at baseline (D0) of
naive unprotected mice and protected mice, 56 days after primary infection,
including time frames of lung clearance, systemic T-helper subsets, serum IgG
profile, mucosal IgA, and pulmonary transcriptomic profile as obtained from data in the
current study and data adapted from our previous study (10). 320 genes
are differential expressed in protected mice compared to naive (D0 unprotected)
mice. (C) An overrepresentation analysis was performed using DAVID for Keywords,
KEGG-pathways, and gene ontology biological pathways (GO-BP) to determine the
function of the 320 genes. For each term, the number of upregulated (red) and
downregulated (green) genes are depicted
Figure 3. Pulmonary gene expression profiles of membrane proteins and secreted proteins in protected and unprotected mice following B. pertussis challenge.
No full textExpression profiles of genes in the lungs encoding
membrane proteins (left panel) and secreted proteins (right panel) were selected
according to GO-BP terms, KEGG pathways and text mining. Each list is divided
in four groups (A-D) based on their expression profile on 4 hours and 2 days p.c.
Group A contains genes that are significantly upregulated in unprotected mice,
but unaltered in protected mice. Group B covers genes that are not upregulated
on 4 hours and 2 days p.i. in unprotected mice, but are directly activated in
protected mice. Group C comprehends genes that are upregulated on 4 hours
and/or 2 days p.c. in both protected and unprotected mice. Group D includes
genes that are significantly regulated from 7 days p.c. in both protected and
unprotected mice. In addition, the color codes of the six clusters from Figure 2 are added
Figure 2. Pulmonary gene expression profiles in protected and unprotected mice following a B. pertussis challenge.
No full text(A) Fold changes in gene expression of both
unprotected and protected mice were calculated compared to naive mice (D0
unprotected). The expression results (FR≥1.5, p-value≤0.001) are visualized as
heatmap (mean of n = 3). Genes not exceeding a fold change of 1.5 are depicted
as basal level (black) at this time point. In total, 786 genes were found to be
differentially regulated. Genes were divided in six clusters (I-VI) based on
their expression profiles (color coding for these clusters is depicted in an
additional table): cluster I (Differential expression in unprotected mice,
absent in protected mice), Cluster II (Differential expression in unprotected
mice and protected mice), Cluster III (Differential expression in unprotected
mice and in protected mice before and after challenge), Cluster IV
(Differential expression in unprotected mice and additional differential
expression as result of challenge in protected mice), Cluster V (Absent in
unprotected mice but differential expression in protected mice) and Cluster VI
(absent in unprotected mice but differential expression pre- and post-challenge
in protected mice). (B) Transcriptomic profiles obtained on 2 days p.i. in
unprotected and 2 days p.c. protected mice were compared by plotting all 786
genes in a scatter plot and divide the genes in different fractions based on
co-expression. The black solid lines are the thresholds for the significant FR
(FR ≥ 1.5 or ≤ 0.67) compared to naive mice (D0 unprotected) for both
unprotected and protected mice. Black dots represent genes that are not
significantly regulated compared to naive mice (D0 unprotected) in both groups.
The red solid lines represent the threshold for the significant FR (FR ≥ 1.5 or
≤ 0.67) of both unprotected 2 days p.i. and protected mice 2 days p.c. All red
triangles represent genes that show significant differential expression (FR ≥ 1.5 or ≤ 0.67) between unprotected 2 days
p.i. and protected mice 2 days p.c. In total, 212
genes were differentially expressed between both groups of which 108 genes were
upregulated and 104 were downregulated. These genes were divided in eight
fractions that are significantly up-regulated (1-4) or downregulated (5-8) in
protected mice compared to unprotected mice and are further specified as
heatmaps in Supplementary
Figure 1B. Dots with other colors (orange, green, brown, and
blue) represent genes that are significantly regulated in unprotected and/or
protected mice compared to naive mice (D0 unprotected) but these genes are not
differentially regulated between unprotected 2 days p.i. and protected mice 2
days p.c. (C-D) A selection of eight terms (KEGG-pathways and GO-BP terms)
found enriched in the ORA of the 786 genes and the kinetics over time of indicated
terms is depicted. (C) Kinetics was determined by averaging the FR for each
term at each time point and is expressed on LN-scale. (D) For each enriched term,
the Benjamini score and the number of upregulated (red) and downregulated
(green) genes in the protected mice and unprotected mice is shown
Measurements of the mass and width of W boson from e"+e"- -> W"+W"- -. (e/#mu#)#nu#qq"- events with the ALEPH detector
No full textIn 1998 at CERN (Geneva), 174 pb"-"1 of data was collected with the ALEPH detector, at an e"+e"- centre-of-mass energy of 188.6 GeV. For this dataset, the selection of semi-leptonic decays of W boson pairs (WW #-># (e/#mu#)#nu#qq-bar) is improved. The resulting efficiencies for selecting e#nu#qq-bar and #mu##nu#qq-bar events are 82.0% and 88.1% respectively, with purities of 92.4% and 93.3%. For the first time in ALEPH, the width of the W boson is measured using a two parameter (mass M_W and width #GAMMA#_W) fit to the reconstructed W mass distribution, yielding: M_W = 80.313 #+-#0.119(stat.) #+-# 0.052(syst.) GeV/c"2; #GAMMA#_W 2.17_-_0_._2_6_"+"0"."2"9"(stat.) #+-# 0.11(syst.) GeV/c"2, for e#nu#qq-bar and #mu##nu#qq-bar decays combined. The mass value is in good agreement with the world average mass and the width value is in good agreement with Standard Model calculations. The systematic error includes detector uncertainties as well as uncertainties in the background, the beam energy, and the theoretical model. Additional studies are done with respect to previous years to take into account errors in the angular and energy resolutions of the ALEPH detector. The stability of the data fit results is checked as a function of various selection cuts. A preliminary measurement is performed at centre-of-mass energies ranging from 191.6 to 201.6 GeV. These results are combined with the result above to obtain the preliminary result for a total integrated luminosity of 411 pb"-"1: M_W = 80.537 #+-# 0.079 #+-# 0.052 GeV/c"2; #GAMMA#_W = 2.11_-_0_._1_8"+"0"."2"0 #+-# 0.11 GeV/c"2. (author)Available from British Library Document Supply Centre-DSC:DXN038352 / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo
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