46 research outputs found
Functional variants in the B-cell gene BANK1 are associated with systemic lupus erythematosus
No full textKozyrev, S.V., Abelson, A.-K., Wojcik, J., Zaghlool, A., Linga Reddy, M.V.P., Sanchez, E., Gunnarsson, I., Svenungsson, E., Sturfelt, G., Jönsen, A., Truedsson, L., Pons-Estel, B.A., Witte, T., D'Alfonso, S., Barrizzone, N., Danieli, M.G., Gutierrez, C., Suarez, A., Junker, P., Laustrup, H., González-Escribano, M.F., Martin, J., Abderrahim, H., Alarcón-Riquelme, M.E
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE QUANTITATIVE DETERMINATION OF ORGANIC IMPURITIES OF DOCETAXEL IN PARENTERAL FORMULATION OF DOCETAXEL USING UV DETECTOR
No full textObjective: The objective is to develop a novel, rapid, simple, precise, accurate, and reproducible reverse-phase high-performance liquid chromatography (RP-HPLC) method for quantitative estimation of organic impurities of docetaxel (DTX) parenteral formulation through high-performance liquid chromatography (HPLC).
Methods: Finalized chromatographic conditions were used for a reversed-phase C18 column with particle size of 3 μm and dimension of 4.6×150 mm, water as mobile phase-A and acetonitrile mobile phase-B. The flow rate is 1.2 mL/min with gradient elution and ultraviolet (UV) detection at 232 nm. Acetonitrile: water:glacialcetic acid in the ratio of 100:100:0.1 (v/v/v) as diluent.
Results: Analytical test method for the quantitative determination of organic impurities of DTX in parenteral formulation of DTX using HPLC with UV detector was verified and found to be linear over the tested concentration range for all impurities (10-deacetylbaccatin: 0.032–0.466 μg/mL; DTX: 0.015–0.151 μg/mL; 6-oxodocetaxel: 0.023–2.080 μg/mL; 4-epidocetaxel: 0.022–1.380 μg/mL; 4-epi-6-oxodocetaxel: 0.021–0.673 μg/mL). The calibration charts plotted were linear with a regression coefficient of R2>0.999. Method-precise results were found to be within the acceptance criteria. Limit of detection and limit of quantification for the active ingredients and their impurities were established with respect to test concentration.
Conclusion: It was concluded that the method is simple, sensitive, precise, and accurate and hasthe ability to separate the drug from degradation products and excipients found in the dosage form
Ultrasonic measurements in ethylacetate and n-butanol
No full text13-19The ultrasonic velocity and
density measurements have been carried out at 2MH frequency in the binary mixture
of ethyl acetate and n-butanol over the entire range of mole fractions at 30
and 40°C temperature. The Pulse echo overlap (PEO) technique is used for
measuring the ultrasonic velocities (v). The measured values of velocity
and density are utilised to compute the
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parameters such as Lf, V and Ks, for an
understanding of their intermolecular interaction.</span
The Genetics of Systemic Lupus Erythematosus : The Specificity of IRF5 to SLE.
No full textThe breakdown of self-tolerance is the main driving force behind susceptibility to SLE. When this occurs, T and B cells are activated in an uncontrolled manner and produce autoantibodies against self fragmented DNA, RNA and sometimes other parts of the cell such as cardiolipin, phosphatidylserine, etc. The mechanism behind the breakdown of self-tolerance may be genetic factors that are triggered by environmental factors. SLE is not caused by a single gene, but by many genes, and is thus a polygenic disease. So far only a few genes have been found to be associated with SLE including PDCD1, FcγRs, and PTPN22. The main aim of my thesis is to find susceptibility genes responsible for SLE. Recently, a gene called IRF5 was found to be associated with SLE. In paper one, we performed a thorough study and confirmed its association to SLE. In addition, we found a few other SNPs in the gene that were associated to the disease. Among them, SNP rs2004640 is very strongly associated and was found to affect the splicing of the gene. Another SNP, rs2280714, correlated with overexpression of the gene, although SNP rs10954213 was much more highly correlated with expression adding to this, in paper two we found a few other SNPs that were associated to SLE and played crucial roles in gene function. An indel in exon 6, though not associated by itself, regulated which isoforms were expressed. Individuals with 2 repeats expressed isoforms V1 and V4, while individuals with 4 repeats expressed isoforms V5 and V6. SNP rs2070197 was also very strongly associated, but did not have a functional role. In paper three, the same polymorphisms were studied in a Mexican population, which showed an even stronger association when compared to a European population. It is known that autoimmune diseases share susceptibility genes, therefore we wanted to see if the IRF5 gene is associated with any other autoimmune diseases. In papers four and five, we tested its association to RA (using three sets of patients and controls from Sweden, Argentina and Spain) and psoriasis (using a set of patients and controls from Sweden). Association was not found in either of the diseases. Therefore, we believe that this association may be SLE-specific
Functional variants in the B-cell gene BANK1 are associated with systemic lupus erythematosus
No full textSystemic lupus erythematosus (SLE) is a prototypical autoimmune disease characterized by production of autoantibodies and complex genetic inheritance. In a genome-wide scan using 85,042 SNPs, we identified an association between SLE and a nonsynonymous substitution (rs10516487, R61H) in the B-cell scaffold protein with ankyrin repeats gene, BANK1. We replicated the association in four independent case-control sets (combined P = 3.7 x 10(-10); OR = 1.38). We analyzed BANK1 cDNA and found two isoforms, one full-length and the other alternatively spliced and lacking exon 2 (Delta2), encoding a protein without a putative IP3R-binding domain. The transcripts were differentially expressed depending on a branch point-site SNP, rs17266594, in strong linkage disequilibrium (LD) with rs10516487. A third associated variant was found in the ankyrin domain (rs3733197, A383T). Our findings implicate BANK1 as a susceptibility gene for SLE, with variants affecting regulatory sites and key functional domains. The disease-associated variants could contribute to sustained B cell-receptor signaling and B-cell hyperactivity characteristic of this disease
Single-channel speech enhancement using implicit Wiener filter for high-quality speech communication
Get PDFSpeech enables easy human-to-human communication as well as human-to-machine interaction. However, the quality of speech degrades due to background noise in the environment, such as drone noise embedded in speech during search and rescue operations. Similarly, helicopter noise, airplane noise, and station noise reduce the quality of speech. Speech enhancement algorithms reduce background noise, resulting in a crystal clear and noise-free conversation. For many applications, it is also necessary to process these noisy speech signals at the edge node level. Thus, we propose implicit Wiener filter-based algorithm for speech enhancement using edge computing system. In the proposed algorithm, a first order recursive equation is used to estimate the noise. The performance of the proposed algorithm is evaluated for two speech utterances, one uttered by a male speaker and the other by a female speaker. Both utterances are degraded by different types of non-stationary noises such as exhibition, station, drone, helicopter, airplane, and white Gaussian stationary noise with different signal-to-noise ratios. Further, we compare the performance of the proposed speech enhancement algorithm with the conventional spectral subtraction algorithm. Performance evaluations using objective speech quality measures demonstrate that the proposed speech enhancement algorithm outperforms the spectral subtraction algorithm in estimating the clean speech from the noisy speech. Finally, we implement the proposed speech enhancement algorithm, in addition to the spectral subtraction algorithm, on the Raspberry Pi 4 Model B, which is a low power edge computing device
Performance Enhancement of MIMO MC-CDMA System employing Cylically Rotated Complete Complementary Codes
Get PDFWireless communication needs very high data rate and throughput in order to meet increasing demand for multimedia applications. MC-CDMA technique along with multiple input multiple output (MIMO) technique is used to increase data rate and to reduce the channel impairments. Spreading code plays a major role in CDMA technique, Cyclically rotated complete complementary codes (CRCCCs) are used for spreading which have perfect auto-correlation and cross correlation properties. Bit error rate (BER) can be reduced and have other advantages like high data rate, high throughput, reduced inter symbol interference (ISI) and multiple access interference (MAI). MIMO MC-CDMA system employing CRCCCs is designed and simulation results are shown.
DOI: 10.17762/ijritcc2321-8169.150614
ChemInform Abstract: CuI Nanoparticles C—N and C—O Cross Coupling of Heterocyclic Amines and Phenols with Chlorobenzenes.
No full text
