81 research outputs found
Colonization of the intestine with nonpathogenic E. coli atrer birth reduces frewuency of allergies and tepeated infections later in life (after 10 and 20 years)
No full textIn our previous studies, the presence of an orally administered probiotic E.coli strain in the intestine stimulated a serum and local antibidy response
An assessment of the quantum for financial provision of mine closures: a Monte Carlo approach.
No full textIncludes abstract.Includes bibliographical references.Poor mine closure policy threatens both society and the environment. Some of the more prominent threats that have manifested are acid mine drainage, slimes dams and toxic dust from dumps. Current legislation requires that new mines set aside funds to cover their rehabilitation costs at closure
The ELISPOT assay for the detection and enumeration of 00 7 immunoglobulin-, antibody-, and/or cytokine- secreting cells
No full text
Complete genome sequencing and probiotic trait analysis of Lacticaseibacillus rhamnosus LR110, a human stool isolate from the NORDBIOTIC collection
No full textWe present the complete genome of Lacticaseibacillus rhamnosus strain LR110, a human stool isolate from the NORDBIOTIC strain collection. The genome consists of a 2,867,184-bp chromosome with 46.8% GC content. Genomic analysis revealed genes related to thiamine salvage, lactose metabolism, and putrescine biosynthesis, providing insight into the strain’s potential probiotic properties
Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ-free piglets: Production by CD5- B cells
No full textPig fetuses, colostrum-deprived newborns and germ-free (GF) piglets, animals in which B-cell development is not influenced by maternal regulatory factors, were employed to study the occurrence and specificity of natural antibodies (NAb). Serum immunoglobulins of all isotypes were found in 44-day-old fetuses (the gestation period in pigs lasts 114 days) and their level, with predominating IgM, was increased during fetal ontogeny. In sera of fetuses at the end of embryonic life as well as of newborns and older GF piglets, antibody activity against autoantigens (thyroglobulin, hormones, ssDNA), phylogenetically conserved proteins (myosin), haptens (trinitrophenyl; TNP) and bacterial components (Escherichia coli O86, tetanic anatoxin) was detected by enzyme-linked immunosorbent assay. The antigen-binding activity of IgM NAb increased after isolation of the serum immunoglobulins on a Staphylococcus Protein A (SPA)–Sepharose column. IgM reactivity similar to that detected in serum was found in supernatants from polyclonally stimulated cultures of spleen of 8- and 12-day-old GF piglets. Pig fetal liver IgM+ B cells, which were able to produce IgM after polyclonal stimulation, did not express the CD5 molecule. Our results indicate that pig preimmune repertoire is comparable to that described in humans and mice, although in contrast to these species pig B-1 cells do not express CD5
Fine mapping of the celiac disease-associated LPP locus reveals a potential functional variant.
No full textUsing the Immunochip for genotyping, we identified 39 non-human leukocyte antigen (non-HLA) loci associated to celiac disease (CeD), an immune-mediated disease with a worldwide frequency of ∼1%. The most significant non-HLA signal mapped to the intronic region of 70 kb in the LPP gene. Our aim was to fine map and identify possible functional variants in the LPP locus. We performed a meta-analysis in a cohort of 25 169 individuals from six different populations previously genotyped using Immunochip. Imputation using data from the Genome of the Netherlands and 1000 Genomes projects, followed by meta-analysis, confirmed the strong association signal on the LPP locus (rs2030519, P = 1.79 × 10-49), without any novel associations. The conditional analysis on this top SNP-indicated association to a single common haplotype. By performing haplotype analyses in each population separately, as well as in a combined group of the four populations that reach the significant threshold after correction (P < 0.008), we narrowed down the CeD-associated region from 70 to 2.8 kb (P = 1.35 × 10-44). By intersecting regulatory data from the ENCODE project, we found a functional SNP, rs4686484 (P = 3.12 × 10-49), that maps to several B-cell enhancer elements and a highly conserved region. This SNP was also predicted to change the binding motif of the transcription factors IRF4, IRF11, Nkx2.7 and Nkx2.9, suggesting its role in transcriptional regulation. We later found significantly low levels of LPP mRNA in CeD biopsies compared with controls, thus our results suggest that rs4686484 is the functional variant in this locus, while LPP expression is decreased in CeD
Complete genome sequence of Limosilactobacillus reuteri LU150, a potential vitamin B 12 producer from the NORDBIOTIC collection
No full textWe performed whole-genome sequencing of Limosilactobacillus reuteri
LU150, a human fecal isolate from the NORDBIOTIC collection. The genome consists
of 2,039,406 bp with a GC content of 38.9%. The genetic content of LU150 suggests its
probiotic potentia
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