304 research outputs found
X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (<it>Ovis aries</it>) and European red deer (<it>Cervus elaphus</it>)
No full textAbstract Background Simple and precise methods for sex determination in animals are a pre-requisite for a number of applications in animal production and forensics. However, some of the existing methods depend only on the detection of Y-chromosome specific sequences. Therefore, the abscence of a signal does not necessarily mean that the sample is of female origin, because experimental errors can also lead to negative results. Thus, the detection of Y- and X-chromosome specific sequences is advantageous. Results A novel method for sex identification in mammals (sheep, Ovis aries and European red deer, Cervus elaphus) is described, using a polymerase chain reaction (PCR) and sequencing of a part of the amelogenin gene. A partial sequence of the amelogenin gene of sheep and red deer was obtained, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. With a specific pair of primers a DNA fragment of different length between the male and female mammal was amplified. Conclusion PCR amplification using the amelogenin gene primers is useful in sex identification of samples from sheep and red deer and can be applied to DNA analysis of micro samples with small amounts of DNA such as hair roots as well as bones or embryo biopsies.</p
P5005 Hitchhiking effects influence allele frequencies and exclusion probabilities of microsatellites used for parentage control in Holstein Friesian cattle
No full text
Cellular nucleic acids in serum and plasm as new diagnostic tools
No full textCurrently, the diagnosis of bovine spongiform encephalopathy is only possible in the brain stem of dead animals. Protease resistant prions are detected in the obex region of the brain stem. However, from a veterinary medical and agricultural point of view the development of an in vivo detection assay is of utmost importance. Because infectious prions are detectable relatively late in the central nervous systeme during an infection, efforts are made searching for surrogate markers. Besides neuronal proteins that are released into the liquor and blood during neurodegenerative processes or other neuronal diseases, cellular nucleic acids circulating in the plasm or serum are an absolutely new approach for the detection of infectious diseases
Cellular nucleic acids in serum and plasm as new diagnostic tools
No full textCurrently, the diagnosis of bovine spongiform encephalopathy is only possible in the brain stem of dead animals. Protease resistant prions are detected in the obex region of the brain stem. However, from a veterinary medical and agricultural point of view the development of an in vivo detection assay is of utmost importance. Because infectious prions are detectable relatively late in the central nervous systeme during an infection, efforts are made searching for surrogate markers. Besides neuronal proteins that are released into the liquor and blood during neurodegenerative processes or other neuronal diseases, cellular nucleic acids circulating in the plasm or serum are an absolutely new approach for the detection of infectious diseases
Glutamicibacter creatinolyticus strain LGCM 259 chromosome, complete genome
No full textNCBI Reference Sequence: NZ_CP034412.1
The complete genome sequencing of G. creatinolyticus LGCM 259 was deposited with the
National Center for Biotechnology Information (NCBI) under accession number CP034412; BioProject:
PRJNA507728, BioSample: SAMN10502625, Assembly: GCF_006094275.1 and the same became NCBI
RefSeq sequence NZ_CP034412.1. https://www.ncbi.nlm.nih.gov/nuccore/1679400616.
LOCUS NZ_CP034412 3309128 bp DNA circular CON 08JUN2019
DEFINITION Glutamicibacter creatinolyticus strain LGCM 259 chromosome,
complete genome.
ACCESSION NZ_CP034412
VERSION NZ_CP034412.1
DBLINK BioProject: PRJNA224116
BioSample: SAMN10502625
Assembly: GCF_006094275.1
KEYWORDS RefSeq.
SOURCE Glutamicibacter creatinolyticus
ORGANISM Glutamicibacter creatinolyticus
Bacteria; Actinobacteria; Micrococcales; Micrococcaceae;
Glutamicibacter.
REFERENCE 1 (bases 1 to 3309128)
AUTHORS Santos,R.G., Silva,A.L., Seyffert,N., Castro,T.L.P., Attili,A.R.,
Rifici,C., Mazzullo,G., Brenig,B., Venanzi,F. and Azevedo,V.
TITLE Complete Genome Sequence of Glutamicibacter creatinolyticus strain
LGCM259,isolated from an abscess of a 12yearold
mare in Italy
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3309128)
AUTHORS Santos,R.G., Silva,A.L., Seyffert,N., Castro,T.L.P., Attili,A.R.,
Rifici,C., Mazzullo,G., Brenig,B., Venanzi,F. and Azevedo,V.
TITLE Direct Submission
JOURNAL Submitted (07DEC2018)
General Biology, UFMG, Av Antonio Carlos
6627, Pampulha, Belo Horizonte, Minhas Gerais 31270901,
Brazil
COMMENT REFSEQ INFORMATION: The reference sequence was derived from
CP034412.
The annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (PGAP). Information about PGAP can be found here:
https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
Bacteria and source DNA available from GcLGCM259.
##GenomeAssemblyDataSTART##
Assembly Date :: OCT2018
Assembly Method :: SPAdes v. 3.9.1
Assembly Name :: GcLGCM259
Genome Representation :: Full
Expected Final Version :: Yes
Genome Coverage :: 286.0x
Sequencing Technology :: Illumina HiSeq
##GenomeAssemblyDataEND##
##GenomeAnnotationDataSTART##
Annotation Provider :: NCBI RefSeq
Annotation Date :: 06/07/2019 10:17:37
Annotation Pipeline :: NCBI Prokaryotic Genome
Annotation Pipeline (PGAP)
Annotation Method :: Bestplaced
reference protein
set; GeneMarkS2+
Annotation Software revision :: 4.8
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes (total) :: 3,035
CDSs (total) :: 2,962
Genes (coding) :: 2,882
CDSs (with protein) :: 2,882
Genes (RNA) :: 73
rRNAs :: 4, 4, 4 (5S, 16S, 23S)
complete rRNAs :: 4, 4, 4 (5S, 16S, 23S)
tRNAs :: 58
ncRNAs :: 3
Pseudo Genes (total) :: 80
CDSs (without protein) :: 80
Pseudo Genes (ambiguous residues) :: 0 of 80
Pseudo Genes (frameshifted) :: 31 of 80
Pseudo Genes (incomplete) :: 51 of 80
Pseudo Genes (internal stop) :: 6 of 80
GenBank
Due to the large size of this record, sequence and annotated features are not shown. Use the "Customize view" panel to change the display##GenomeAnnotationDataEND##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..3309128
/organism="Glutamicibacter creatinolyticus"
/mol_type="genomic DNA"
/strain="LGCM 259"
/isolation_source="abcess of a mare"
/host="horse"
/db_xref="taxon:162496"
/country="Italy"
/collection_date="2015"
CONTIG join(CP034412.1:1..3309128)
/
Diagnostic polymorphisms in the mitochondrial cytochrome b gene allow discrimination between cattle, sheep, goat, roe buck and deer by PCR-RFLP
No full textBackground: As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. Results: Interspecies-specific DNA sequence polymorphisms in the mitochondrial cytochrome b gene were analyzed using PCR-RFLP technology to determine the source (i.e., species) of blood traces obtained from a leaf. Conclusions: The method presented can be used for the discrimination of cattle (Bos taurus), sheep (Ovis aries), goat (Capra hircus), roe buck (Capreolus capreolus) and red deer (Cervus elaphus)
Analysis and mapping of CACNB4, CHRNA1, KCNJ3, SCN2A and SPG4, physiological candidate genes for porcine congenital progressive ataxia and spastic paresis
No full textThe cause of porcine congenital progressive ataxia and spastic paresis (CPA) is unknown. This severe neuropathy manifests shortly after birth and is lethal. The disease is inherited as a single autosomal recessive allele, designated cpa. In a previous study, we demonstrated close linkage of cpa to microsatellite SW902 on porcine chromosome 3 (SSC3), which corresponds syntenically to human chromosome 2. This latter chromosome contains ion channel genes (Ca(2+), K(+) and Na(+)), a cholinergic receptor gene and the spastin (SPG4) gene, which cause human epilepsy and ataxia when mutated. We mapped porcine CACNB4, KCNJ3, SCN2A and CHRNA1 to SSC15 and SPG4 to SSC3 with the INRA-Minnesota porcine radiation hybrid panel (IMpRH) and we sequenced the entire open reading frames of CACNB4 and SPG4 without finding any differences between healthy and affected piglets. An anti-epileptic drug treatment with ethosuximide did not change the severity of the disease, and pigs with CPA did not exhibit the corticospinal tract axonal degeneration found in humans suffering from hereditary spastic paraplegia, which is associated with mutations in SPG4. For all these reasons, the hypothesis that CACNB4, CHRNA1, KCNJ3, SCN2A or SPG4 are identical with the CPA gene was rejected
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