6 research outputs found
Strong interfacial polarization in graphene/ZnO nanocomposite for high-performance miniscule permittivity materials
No full textMORPHOMETRIC VARIATIONS BETWEEN TRIPLOID AND DIPLOID Clarias gariepinus (Burchell, 1822)
No full textNekoliko znanstvenih metoda ranije je opisano za
prepoznavanje triploidnih riba. Međutim, mnoge od tih
metoda nisu primjenjive u svrhu rutinskog upravljanja radi
njihove složenosti i troškova. U ovom radu istraživana je
mogućnost korištenja morfoloških varijacija kao jeftine i
jednostavne metode razlikovanja triploidnih i diploidnih
afričkih somova Clarias gariepinus (Burchell, 1822).
Triploidski somovi su proizvedeni metodom hladnog šoka
oplođenih jaja na 5°C tijekom 20 minuta (približno 3 min.
nakon oplodnje). Ribe su bile inkubirane, izvaljene i uzgajane
tijekom 3 mjeseca. Plodnost riba utvrđena je promatranjem
oblika eritrocita. Triploidni eritrocit bio je elipsoidnog oblika
dok je diploidi bio okrugli. Morfološka karakterizacija je
provedena na 100 uzoraka triploidnih i na 100 uzoraka
diploidnih afričkih somova. Iako su značajne razlike
zabilježene u mnogim parametrima, glavna morfometrijska
razlika između triploidnih i diploidnih afričkih somova nije se
mogla jasno razlikovati. Stoga je zaključeno da morfološka
svojstva nisu idealna za razlikovanje triploida i diploida
afričkog soma. Upotreba eritrocitnih karakteristika i dalje je
najjeftiniji i relativno učinkovit način za triploido i diploidno
određivanje afričkih somova.Several scientific methods have been described in the identification of
triploid fish. However, many of these methods are not applicable for routine
management purposes due to their complexity and cost. In this study, the
possibility of using morphological variation as a least cost and less complex
method of distinguishing triploid and diploid African catfish Clarias
gariepinus (Burchell, 1822) was examined. Triploid catfish were produced
by cold shock of fertilized eggs in 5°C for 20 mins (at approximately 3
mins after fertilization). The fish were incubated, hatched and raised for
3 months. Ploidy levels of the fish were then ascertained by observing
the erythrocyte shape. Triploid erythrocyte was ellipsoidal in shape while
diploid was round. Morphological characterization was then carried out on
100 samples each of triploid and diploid African catfish. Although significant
differences were observed in many parameters, the principal morphometric
difference between triploid and diploid African catfish could not be clearly
distinguished. It was therefore concluded that morphological characteristics
is not ideal for discriminating triploids and diploids of African catfish. The
used of erythrocyte characteristics still remains the cheapest and relatively
effective method for triploid and diploid determination in African catfish
Morphometric Variations Between Triploid and Diploid Clarias gariepinus (Burchell, 1822)
No full textSeveral scientific methods have been described in the identification of triploid fish. However, many of these methods are not applicable for routine management purposes due to their complexity and cost. In this study, the possibility of using morphological variation as a least cost and less complex method of distinguishing triploid and diploid African catfish Clarias gariepinus (Burchell, 1822) was examined. Triploid catfish were produced by cold shock of fertilized eggs in 5°C for 20 mins (at approximately 3 mins after fertilization). The fish were incubated, hatched and raised for 3 months. Ploidy levels of the fish were then ascertained by observing the erythrocyte shape. Triploid erythrocyte was ellipsoidal in shape while diploid was round. Morphological characterization was then carried out on 100 samples each of triploid and diploid African catfish. Although significant differences were observed in many parameters, the principal morphometric difference between triploid and diploid African catfish could not be clearly distinguished. It was therefore concluded that morphological characteristics is not ideal for discriminating triploids and diploids of African catfish. The used of erythrocyte characteristics still remains the cheapest and relatively effective method for triploid and diploid determination in African catfish
Genetic Variation between Triploid and Diploid Clarias gariepinus (Burchell, 1822) Using RAPD Markers
No full textThis study was designed to examine the use of RAPD markers in discriminating triploid and diploid African catfish Clarias gariepinus (Burchell, 1822). Following a routine technique, triploidy was induced by cold shock and confirm by erythrocyte measurement in C. gariepinus. Thereafter, 80 RAPD markers were screened; out of which, three showed the highest percentage of polymorphism (i.e., OPB 16 = 71.43%; OPC 14 = 61.9%; OPD 12 = 75%). The results obtained showed genotype differences between triploid and diploid without overlapping. However, the development of a Sequence Characterized Amplified Region (SCAR) marker was not achievable because progenies of triploid and diploid C. gariepinus could not be differentiated based on a specific fragment. Consequently, the genetic distance showed high similarities for both treatments and the UPGMA-generated dendrogram could not separate the treatments into two distinct clusters. It was concluded that RAPD makers cannot be used to separate the ploidy status of fishes
Crigler-Najjar Syndrome Type 2 in a Young Adult
No full textCrigler-Najjar syndrome type 2 in an autosomal recessive congenital non-hemolytic hyperbilirubinemia caused by UDP-glucuronosyltransferase deficiency. Only a few hundred cases have been described in the literature so far. We are reporting Crigler-Najjar syndrome type 2 in an 18 year old female born out of consanguineous marriage. Keyword: Crigler-Najjar syndrome; UDP-glucuronosyltransferase; Bangladesh DOI: 10.3329/jom.v12i1.6359J Medicine 2011; 12 : 81-85</jats:p
Morphometric Variations Between Triploid and Diploid<i>Clarias gariepinus</i>(Burchell, 1822)
No full textAbstractSeveral scientific methods have been described in the identification of triploid fish. However, many of these methods are not applicable for routine management purposes due to their complexity and cost. In this study, the possibility of using morphological variation as a least cost and less complex method of distinguishing triploid and diploid African catfishClarias gariepinus(Burchell, 1822) was examined. Triploid catfish were produced by cold shock of fertilized eggs in 5°C for 20 mins (at approximately 3 mins after fertilization). The fish were incubated, hatched and raised for 3 months. Ploidy levels of the fish were then ascertained by observing the erythrocyte shape. Triploid erythrocyte was ellipsoidal in shape while diploid was round. Morphological characterization was then carried out on 100 samples each of triploid and diploid African catfish. Although significant differences were observed in many parameters, the principal morphometric difference between triploid and diploid African catfish could not be clearly distinguished. It was therefore concluded that morphological characteristics is not ideal for discriminating triploids and diploids of African catfish. The used of erythrocyte characteristics still remains the cheapest and relatively effective method for triploid and diploid determination in African catfish.</jats:p
