278 research outputs found
Woodside Energy Ltd. Cossack Pioneer Facility Engineering Team
No full textCossack Pioneer is a floating production storage and offloading vessel located 112 km North West of Karratha. This report details the work performed during a 16 week internship with Woodside Energy Ltd working in the Cossack Pioneer Facility Engineering Team. This Perth based team provides engineering support to the production facility. The report incorporates a description of the facility and topsides process and discusses the systems used for process control.
The earlier work performed during the internship focussed on small engineering design and control system modifications for the instrumentation and control group within the facility engineering team. Partway through the internship focus changed and the challenging role of Facility Control Engineer for Cossack Pioneer was assumed during the absence of the facility Senior Control Engineer. The report provides discussion of learning outcomes acheived and experience gained during the internship
sj-docx-1-aph-10.1177_10105395231151284 – Supplemental material for Active and Passive Smoking in Adults Undergoing a Long-Term Multi-Setting Healthy Lifestyle Education: Tehran Lipid and Glucose Study
No full textSupplemental material, sj-docx-1-aph-10.1177_10105395231151284 for Active and Passive Smoking in Adults Undergoing a Long-Term Multi-Setting Healthy Lifestyle Education: Tehran Lipid and Glucose Study by Hasti Masihay-Akbar, Parisa Amiri, Leila Cheraghi, Amir Abbas Momenan and Fereidoun Azizi in Asia Pacific Journal of Public Health</p
Corrigendum to Developing a Sustainability Indicator Set for Measuring Green Infrastructure Performance
No full textThere was only one correction in corresponding author. It should be Parisa Pakzad
Living alpha-olefin polymerization by cationic zirconium and hafnium complexes containing chelating diamidopyridine ligands
No full textThesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2002.Vita.Includes bibliographical references (leaves 196-199).This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.by Parisa Mehrkhodavandi.Ph.D
Known and potential molecules associated with altered B cell development leading to predominantly antibody deficiencies
No full textPredominantly antibody deficiencies (PADs) encompass a heterogeneous group of disorders characterized by low immunoglobulin serum levels in the presence or absence of peripheral B cells. Clinical presentation of affected patients may include recurrent respiratory and gastrointestinal infections, invasive infections, autoimmune manifestations, allergic reactions, lymphoproliferation and increased susceptibility to malignant transformation. In the last decades, several genetic alterations affecting B cell development/maturation have been identified as causative of several forms of PADs, adding important information on the genetic background of PADs which in turn should lead to a better understanding of these disorders and precise clinical management of affected patients. This review aims to present a comprehensive overview of the known and potentially involved molecules in the etiology of PADs to elucidate the pathogenesis of these disorders and eventually offer a better prognosis for affected patients
Fabbisogni energetici per edifici caratterizzanti il terziario in Italia: aspetti termici ed illuminotecnici
No full textIl presente lavoro verte sull'individuazione di benchmark di fabbisogno energetico per edifici di riferimento con destinazione d'uso non-residenziale. In particolare, si intende fornire profili tipo di domanda energetica per l'illuminazione artificiale, al fine di valutarne l'effetto sui profili di fabbisogno di energia per la climatizzazione estiva ed invernale, nonché di individuare le variabili che maggiormente incidono sulla loro determinazione. Per giungere a questi risultati, ci si avvale dell'uso di programmi informatici di simulazione dinamica riconosciuti ed impiegati a livello scientifico, in ambito europeo ed extraeuropeo (Energy Plus). La ricerca viene condotta sotto vari aspetti: architettonico, energetico e ambientale. Il primo obiettivo, partendo dalle indicazioni bibliografiche, legislative e normative, è quello di caratterizzare in modo univoco gli edifici di riferimento per le varie destinazioni d'uso oggetto di analisi: edifici per l'istruzione, ed edifici terziari. Gli edifici di riferimento intendono costituire archetipi rappresentativi del patrimonio edilizio italiano. Il secondo obiettivo è di tipo energetico: si intende definire profili tipo di domanda energetica per l'illuminazione artificiale degli ambienti. Si intendono inoltre valutare contestualmente i fabbisogni energetici netti per il riscaldamento e il raffrescamento degli ambienti. L'obiettivo finale è riuscire a determinare le migliori soluzioni progettuali per la minimizzazione del fabbisogno di energia connesso all'illuminazione, in relazione alle destinazioni d'uso dei differenti edifici, nonché le variabili che più incidono per indirizzare al meglio le attenzioni progettuali in caso di nuova realizzazione. Il presente lavoro, dopo un primo inquadramento generale in cui si delinea quello che è lo stato dell'arte della valutazione dei fabbisogni energetici e della ricerca scientifica in materia di illuminazione artificiale di ambienti non residenziali, passa poi ad indagare il significato di archetipo e a definire degli archetipi edilizi per il patrimonio immobiliare costruito a destinazione d'uso scolastica e terziaria. Successivamente vengono descritti i modelli di calcolo impiegati e sono dettagliati i dati di input impiegati per la loro definizione. Sono infine esplicati i risultati ottenuti, con individuazione dei valori di benchmark energetico per gli archetipi edilizi scolastici e terziari, e con l'analisi di quelle che sono le grandezze maggiormente influenti sul fabbisogno energetico per illuminazion
Development of blast disease-resistant transgenic rice variety from MR219 through transformation with Pikh gene
No full textFood security has become a concern of global importance, and major price spike of staple food crops, such as rice has occurred in recent years. These price spikes
are partly due to the brunt of plant disease. Magnapor the oryzae, rice blast fungus, is a plant pathogen causes a serious rice disease and therefore poes a threat to the world‟s important food security crop. Planttrans formation technology has become an adaptable system for cultivar development and also for functional analysis of gene in plants. The objectives of this study were (i) to
screen Malaysian rice varieties phenotypically, physiologically and genetically against blast disease (ii) to isolate Pikh gene from leaves of resistant rice variety (PH9), (iii) to construct the over-expression vector carrying CDS of Pikhgene,and (iv) to determine the effects of (over-expression)of Pikh in MR219 rice
variety. Sixteen important Malaysian rice varieties were screened phenotypically (by scoring), physiologically (measuring photosynthesis and its components) and
genetically (gene expression analysis using Real-Time PCR). Specific primer was designed to isolate full Coding DNA Sequence (CDS) of Pikh gene from PH9 rice variety. Entry and the expression vectors were constructed using the Gateway Technology. Agrobacterium-mediated transformation technology was used to introduce Pikh gene to the callus of MR219. Transgenic plants were evaluated from DNA to protein stages by polymerase chain reaction (PCR),Semi-quantitative RT-PCR, Real-Time PCR, high performance liquid chromatography. Transgenic plants also were compared to the control plants using Real-Time quantification technique (to quantify pathogen population) and also challenging of transgenic and control plants with the local most virulent M.
oryzaepathotype, P7.2. The TRIzol method was quick and reliable method for RNA extraction from leaves of rice. Ten out of 16 varieties (MR159, PH9,MR84, MR185, MR253, MR269, MRQ74, MRQ50, Pulut Siding and Pongsu Seribu 2) demonstrated high degree of resistance to pathotype P7.2 rice leaf blast. Photosynthesis and chlorophyll contents were decreased significantly among treated susceptible varieties compared to the controls. There were absent of differences in photosynthesis and its components between inoculated and noninoculated resistant varieties. Hence, it seems that energy sources are provided for both resistant and susceptible plants, but the expression of defence-associated genes restricts the pathogens accessibility in the resistant varieties.Our findings
provide evidences that the expression profiling of Pikh, Pi9, Pi21, and Osw45 genes is involved in the defense responses in the leaves of rice 31 h after inoculation of plants by M. oryzae. Full CDS ofPikhgene with 1206 bp length was obtained through amplification of the cDNA template using specific primer. Pikh gene was up-regulated in the transgenic plants in comparison to the control plants. The amount of leucine amino acid of transgenic rice plants has increased significantly from 17.131 in the wild-type to 47.865 mg g-1. The M.oryzae
population was constant at 31, 48 and 72h after inoculation in transgenic plants while itincreased in inoculated control plants. This study successfully clarified that over-expression of Pikh gene in the transgenic plants is able to improve the resistance of rice against M.oryzaepathotype P7.2
Isolating, characterizing, and engineering novel Cu-proteins and peroxidases
No full textMetalloproteins are a fascinating class of proteins that function at the heart of several important biological processes including photosynthesis, respiration, and nitrogen fixation. It is even more amazing, considering that nature uses a small set of tertiary structures and metal centers to perform all these different functions with efficiency and selectivity. How nature tunes the activity within these scaffolds has been the area of research for many years. The goal of this work is to understand the underlying mechanisms of such tuning with a special focus on the role of subtle changes of residues in the secondary coordination sphere of the metal ion, an underexplored area of study. I use protein engineering techniques not only to shed light on the mechanisms underlying such changes, but also to design new functionalities within our scaffold proteins and to enhance their properties for specific purposes, such as fuel generation.
This work is divided into three main sections. In the first, I focus on characterizing a novel metalloprotein, N. mar_1307, from the organism Nitrosopumilus maritimus. While the protein shares a protein fold and Type 1 copper coordination site with other common electron transfer cupredoxins, the lack of an axial residue creates an open binding position in the Cu center, leading to a novel enzymatic function, NO oxidation. The purification, characterization, and activity assays of the protein are described in detail in chapter 2.
The second and major focus of this work is on tuning the reduction potential of azurin, a common electron transfer protein. In chapter 3 I demonstrate that how by making mutations around the Cu site, and replacing Cu with Ni I can obtain an azurin variant with a reduction potential of nearly 1V, the highest potential that can be observed under physiological conditions, along with other variants with negative potentials. Chapter 4 describes the characterization of a series of Phe114 mutants that were used to understand the role of this critical secondary sphere residue in tuning the reduction potential of the Cu site. Chapter 5 demonstrates the Marcus inverted region of electron transfer in a series of azurin variants with different reduction potentials. Finally, I show my initial attempts toward the design of a high-throughput screening platform for the directed evolution of azurin in chapter 6.
In chapters 7 and 8, I focus on the design of novel functionalities in one of our model scaffolds, cytochrome c peroxidase (CcP). Chapter 7 describes the work done to enhance the Mn(II) oxidation activity in a designed model of manganese peroxidase within the CcP scaffold based on modifications of the second coordination sphere around the Mn(II) binding site. In chapter 8 I report the design and characterization of a novel CcP variant that shows catalase-like activity in “as-purified” form and forms a heme-protein crosslink in the heme-bound form.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-12-01The student, Parisa Hosseinzadeh, accepted the attached license on 2015-09-17 at 11:09.The student, Parisa Hosseinzadeh, submitted this Dissertation for approval on 2015-09-17 at 11:57.This Dissertation was approved for publication on 2015-10-08 at 14:46.DSpace SAF Submission Ingestion Package generated from Vireo submission #8686 on 2016-03-02 at 14:11:52Made available in DSpace on 2016-03-02T20:57:17Z (GMT). No. of bitstreams: 2
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Antisense-mediated melanoma inhibitor of apoptosis protein downregulation sensitizes G361 melanoma cells to cisplatin
No full textMalignant melanoma is an aggressive form of skin cancer that is highly resistant to conventional therapies. The melanoma inhibitor of apoptosis protein is a potent inhibitor of apoptosis and is overexpressed in melanoma cells, but undetectable in most normal tissues including melanocytes. We designed 20-mer phosphorothioate antisense oligonucleotides complementary to five putatively single-stranded sites on the melanoma inhibitor of apoptosis protein mRNA and investigated their ability to sensitize G361 melanoma cells to cisplatin. Inhibition of melanoma inhibitor of apoptosis protein mRNA and protein expression were measured by real-time polymerase chain reaction and immunoblotting. Cell viability and apoptosis were quantitated by colorimetric viability assays and by annexin V staining, respectively. Oligonucleotide M706 was identified as the most efficient antisense sequence which downregulated melanoma inhibitor of apoptosis protein mRNA and protein levels in G361 cells by 68 and 78%, respectively. The specificity of target downregulation was confirmed using scrambled sequence control oligonucleotides that only marginally decreased melanoma inhibitor of apoptosis protein expression. Whereas downregulation of melanoma inhibitor of apoptosis protein moderately inhibited cell growth by 26%, in combination with cisplatin, this resulted in a supra-additive effect with almost 57% reduction in G361 cell viability compared with cisplatin alone (17%) (P<0.05). Cell death was mainly due to apoptosis as demonstrated by a 3- to 4-fold increase in annexin V-positive cells and typical morphological changes compared with controls. In summary, we describe a new antisense oligonucleotide that efficiently downregulates melanoma inhibitor of apoptosis protein expression and sensitizes melanoma cells to cisplatin
Correction: Recent Advances in Atmospheric Chemistry of Mercury
No full textThe published paper [1] has been updated to remove instances of copied text from otherpublications [2–6]. Changes have been made throughout the paper, with the most significant alterationsmade in Sections 2.1, 2.4, 3.1, 3.2, 3.3, and 4.The authors wish to provide the following explanation. Ref. [1] is a review article that was invitedfor the journal Atmosphere. The author Lin Si took the lead in writing the article, although this is thefirst time she has undertaken writing a review paper. Sections copied were cited, and a number ofthem were taken from Parisa A. Ariya’s previously published articles. The authors offered to retractthe paper. As scientists we are seeker of the truth, and our integrity and ethics are the most preciousgifts that we leave to future next generations.This Correction was deemed necessary to avoid the impression that the text presented in [1]was the original work of the authors. The Editorial Office accepts that the authors did not intend tomisrepresent the work, however, the original wording did not make it sufficiently clear that a numberof passages were direct quotations.We wish to thank the authors for their cooperation and apologize to readers that this case was notdetected earlier. Atmosphere routinely checks submitted manuscripts for duplication, but issues weremissed in this case due to human error
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