14 research outputs found

    Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family

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    Eisvand, Payam, Nejad, Reza Farrokhi, Azimi, Sedighe (2019): Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family. Zootaxa 4563 (3): 482-490, DOI: 10.11646/zootaxa.4563.3.

    FIGURE 2. Basiria khouzestanensis n in Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family

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    FIGURE 2. Basiria khouzestanensis n. sp. A&B: Female pharyngeal region, C: Lateral field at mid-body, D: Female reproductive system, E&F: Female tail, G&H: Male tail. (Scale bars = 20 µm.)Published as part of Eisvand, Payam, Nejad, Reza Farrokhi & Azimi, Sedighe, 2019, Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family, pp. 482-490 in Zootaxa 4563 (3) on page 486, DOI: 10.11646/zootaxa.4563.3.4, http://zenodo.org/record/260141

    FIGURE 3. Bayesian 50 in Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family

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    FIGURE 3. Bayesian 50% majority rule consensus tree inferred from analysis of the D2–D3 domains of the 28S rRNA gene under the GTR+G+I model. Bayesian posterior probability values more than 50% are given for appropriate clades. The new species is in bold.Published as part of Eisvand, Payam, Nejad, Reza Farrokhi & Azimi, Sedighe, 2019, Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family, pp. 482-490 in Zootaxa 4563 (3) on page 487, DOI: 10.11646/zootaxa.4563.3.4, http://zenodo.org/record/260141

    Basiria khouzestanensis Eisvand & Nejad & Azimi 2019, n. sp.

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    <i>Basiria khouzestanensis</i> n. sp. <p>Figs 1, 2</p> <p> <b>Description.</b> Measurements, see Table 1.</p> <p> <i>Female</i>. Body ventrally arcuate following heat fixation. Cuticle annuli 0.7–1.2 µm wide at mid-body. Lateral field with four incisures, 3.6–4.8 µm wide, occupying 28–30% of body diameter, with non-areolated bands. Lip region smooth and elevated, continuous with body contour, 2.4–3.1 µm high and 4–5.4 µm wide. Amphidial apertures oblique and slit-like. Stylet straight with rounded basal knobs, slightly posteriorly directed, 0.8–1.5 µm across. Dorsal pharyngeal gland orifice (DGO) 4–6 µm posterior to stylet knobs. Median bulb oval, 9–12 µm long and 4–6.6 µm wide, with a weakly developed valve; located at about the middle of the pharynx. Isthmus slender. Basal bulb short and almost pyriform to slightly cylindrical, 6–9.5 µm wide and 12–16.5 µm long. Cardia small. Excretory pore mostly at the level of anterior part of the pharyngeal bulb or rarely at the level of the beginning of the basal bulb, posterior to hemizonid. Nerve ring surrounding the middle part of the isthmus. Reproductive system monodelphic-prodelphic, composed of an outstretched ovary with oocytes arranged in a single row. Spermatheca non-offset, elongated, filled with rounded sperm, 18–36 µm long and 6.5–12 µm wide. Vulva a transverse slit lacking flaps or epiptygma. Vagina 6–7.8 µm long, about one half of body diameter. Post-vulval uterine sac (PUS) 70–77% of corresponding body width in length. Tail elongate-conoid, about equal to the vulva-anus distance, tail terminus variously shaped, mostly slightly rounded to pointed tip in some specimens with a broadly rounded to slightly clavate tip.</p> <p> <i>Male</i>. Less frequent than female and shorter. General morphology similar to that of female, except for character states associated with sexual differences. Testis single, outstretched; Spermatocytes arranged in a single row. Spicules tylenchoid, small and slightly ventrally arcuate. Gubernaculum simple, rod-shaped. Bursa adanal, simple, with smooth margins. Tail similar to that of the female.</p> <p> <b>Type host and locality.</b> Soil samples collected from the rhizosphere of orange (<i>Citrus sinensis</i> L.) in the vicinity of Baghmalek city in Khouzestan Province, GPS coordinates: 31° 32′ 21.5″ N, 49° 50′ ″ E, southwestern Iran.</p> <p> <b>Etymology.</b> The new species name refers to the province name where it was found.</p> <p> <b>Type material.</b> Holotype, 13 females and 8 males paratypes deposited at the nematology laboratory of the Department of Plant Protection, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Two female and one male paratypes deposited at the Wageningen Nematode Collection (WaNeCo), Wageningen, The Netherlands (collection number: WT 3724).</p> <p> <b>Diagnosis and relationships.</b> <i>Basiria khouzestanensis</i> <b>n. sp.</b> is characterized by having short body length (430–635 µm), lateral fields composed of four incisures, with non areolated bands, cephalic region smooth and continuous with body contour, stylet 9–12.5 µm long, basal bulb short and pyriform to slightly cylindrical, V = 63.3 (58–65.5), spermatheca non-offset, tail elongate-conoid, tail tip with various shapes and males with 16.5 (15– 18) µm long spicules.</p> <p> Due to the median bulb located at about the middle of the pharynx and basal bulb pyriform to slightly cylindrical, the new species comes close to <i>B. babhi</i> Siddiqi, 1986, <i>B. brevia</i> (Sultana, 1980) Hashim, 1985, <i>B. graminophila</i> Siddiqi, 1959, <i>B. lauta</i> Randhawa, Khera & Khan, 1996 and <i>B. shahidi</i> Khan, 1982.</p> <p> Compared to <i>B. babhi</i>, the new species has a shorter body (430–635 <i>vs</i> 740–760 µm), lower <i>c</i> ratio (4.9–6.7 <i>vs</i> 8), lower <i>V</i> and <i>V</i> ŕatio (58–65.5 <i>vs</i> 77–78 and 75.1–80 <i>vs</i> 88–89, respectively), tail tip with various shapes (pointed tip and rounded to slightly clavate <i>vs</i> only broadly rounded terminus).</p> <p> Compared to <i>B. brevia</i>, the outer bands of the lateral fields in the new species are smooth (<i>vs</i> distinctly crenate). It also differs in longer body length (430–635 <i>vs</i> 390–420 µm), longer stylet and more posterior DGO (9– 12.5 <i>vs</i> 7.5–8.5 and 4–6 µm <i>vs</i> just behind the stylet base, respectively), basal bulb pyriform to slightly cylindrical (<i>vs</i> pyriform), longer tail (86–120 <i>vs</i> 71–74 µm) and male present (<i>vs</i> unknown).</p> <p> Compared to <i>B. graminophila</i>, the outer bands of the lateral fields in the new species are smooth (<i>vs</i> distinctly crenate), basal bulb pyriform to slightly cylindrical (<i>vs</i> pyriform) and there are remarkable differences in 28S rRNA gene sequences. There was only one record in GenBank for sequence of D2–D3 expansion segments of 28S rRNA gene for <i>B. graminophila</i>. Sequence variation between new species and <i>B. graminophila</i> were 24.6% (133 bp).</p> <p> Compared to <i>B. lauta</i>, the new species has basal bulb pyriform to slightly cylindrical (<i>vs</i> pyriform), higher <i>c</i> ratio (9.3–13.8 <i>vs</i> 5.8–6.6), more posterior DGO (4–6 <i>vs</i> 3.5 µm), tail tip with various shapes (pointed tip and rounded to slightly clavate <i>vs</i> only clavate terminus) and males present (<i>vs</i> unknown).</p> <p> Finally, the new species also resembles <i>B. shahidi</i>, but differs from it by the shorter tail (86–120 <i>vs</i> 134–140 µm), tail tip with various shapes (<i>vs</i> only pointed terminus), and in shorter spicule and gubernaculum lengths (15– 18 vs 21–24 and 3.8–5.5 vs 6–7 µm, respectively).</p> <p> <b>Molecular phylogenetic status.</b> Partial sequencing of the 28S rRNA gene (D2–D3 region) of the new species yielded a fragment of 683 nt. The result of a Blast search using this fragment revealed the highest similarity with three unidentified species of the genus <i>Basiria</i> (JQ004998, JQ004999 and JQ005000) with identities of 87%, 87% and 86%, respectively. A total of 42 isolates of Tylenchidae (ingroup members) and Aphelenchoididae Skarbilovich, 1974 (as outgroup taxa) were selected for LSU rDNA gene (D2–D3 region) phylogenetic analyses. This dataset comprises 822 total characters. The phylogenetic relationships between the new species and representatives of Tylenchidae, as inferred from the Bayesian inference (BI) analysis, are presented in Figure 3. The sequenced isolate from the present study is in boldface.</p> <p> All isolates of <i>Basiria</i> species formed one clade. The monophyly of this clade is highly supported (BPP = 0.99). The new species clusters in a subclade containing three unidentified species of the genus <i>Basiria</i> from Iran (JQ004998, JQ004999 and JQ005000) with high support (BPP = 1.00). <i>B. graminophila</i>, <i>B. gracilis</i> and one unidentified species of the genus <i>Basiria</i> (DQ077803), formed another subclade with high support (BPP = 1.00).</p>Published as part of <i>Eisvand, Payam, Nejad, Reza Farrokhi & Azimi, Sedighe, 2019, Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family, pp. 482-490 in Zootaxa 4563 (3)</i> on pages 483-487, DOI: 10.11646/zootaxa.4563.3.4, <a href="http://zenodo.org/record/2601410">http://zenodo.org/record/2601410</a&gt

    First Morphological and Molecular Characterization of Paratylenchus vandenbrandei (Rhabditida: Tylenchulidae) in Iran

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    Paratylenchus vandenbrandei, has been recovered from the rhizospheric soil of Euphrates poplar (Populus euphratica) in the Karkheh protected area of Khuzestan province, southwestern Iran. The species was identified as P. vandenbrandei by the presence of three lines in the lateral fields; conoid rounded lip region; presence of submedian lobes, a stylet 24.0–28.8 μm long; an excretory pore at the level of the anterior part of the pharyngeal bulb; a round-to-oval spermatheca; presence of vulval flaps; and a conoid tail, with a terminus that is rounded or slightly pointed in some specimens. Males have a conoid tail, with a rounded-to-slightly-pointed terminus. The phylogenetic relationships of the species were reconstructed and investigated using partial sequencing of the D2-D3 expansion segments of large subunits, as well as internal transcribed spacer regions (LSU D2-D3 and ITS rDNA) based on Bayesian inference (BI). P. vandenbrandei has formed a clade with P. neonanus, P. minor, P. nainianus, P. chongqinjensis, P. pedrami, P. baldaccii, P. leptos and P. rostrocaudatus with maximal support (BPP = 1.00). To the best of our knowledge, this is the first report of P. vandenbrandei in Iran and the first molecular characterization of the species worldwide

    DESCRIPTION OF TYLENCHORHYNCHUS IRANENSIS SP. N. (NEMATODA TELOTYLENCHIDAE) FROM IRAN

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    A new species of stunt nematodes, Tylenchorhynchus iranensis sp. n. is described from the rhizosphere of faba bean (Vicia faba L.), from material collected in the Khuzestan province, southwestern Iran. The new species is characterized by the following combination of features: lateral fields with four non areolated incisures, cephalic region continuous to slightly offset and conformed by 6-7 fine annuli, stylet 15-18 µm long, post-anal intestinal sac extends into the entire tail cavity, epiptygma present, tail sub-cylindrical with a hemispherical to sub-hemispherical smooth terminus, 46-65 μm long and composed by 46-49 annuli. Molecular analysis based on 28S rRNA gene sequences placed T. iranensis sp. n. within a clade that contained representatives of the genus Tylenchorhynchus with high support

    Investigating Leisure-Time Patterns of Individuals with Physical Disabilities: A Case Study of Individuals with Physical Disabilities in the City of Kashan

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    Investigating Leisure-Time Patterns of Individuals with Physical Disabilities: A Case Study of Individuals with Physical Disabilities in the City of Kashan Mohsen Shaterian[1]  ,  Sedighe Kiani Salmi[2]  ,  Maryam Kamari[3] Received: 13/05/2018             Accepted: 19/01/2019   Abstract The study of how individuals with disabilities spend their leisure time in order to identify their limitations and weaknesses based on their viewpoints is an essential element in planning. The results will be effective in improving the services and investment with the goal of improving, at least, part of their leisure time quality. The purpose of this study is to identify how individuals with physical disabilities in Kashan spend their leisure time. The research tool for this applied study is a questionnaire with 66 items which was developed after the study of the background and theoretical foundations of the research with an approach to empowering individuals with physical disabilities in managing their leisure time. The tool was validated with the help of experts in the field and its reliability was caluculated using the Cronbach's alpha coefficient to be 0.776. The results of the questionnaire were analyzed using AMOS and SPSS softwares. Findings indicate that home-activities with a factor load of 0.93 had a greater role in the leisure time of individuals with physical disabilities. Socializing with friends with a factor load of 0.77 ranked in second followed by computer activities, mass media, and the family. The results of the study on the problems of individuals with physical disabilities in spending their   leisure time indicate that low safety of sports equipment and lack of proper equipment for exercise with a factor load of 0.90 are the main barriers to performing leisure time activities. Other important obstacles are a lack of leisure facilities such as benches and sports facilities, lack of standard footpaths, adequate floor coverings and parking spaces with regression weights of 0.88, 0.82, 0.81, 0.77 and 0.57, respectively. In order to improve the leisure-time patterns of individuals with physical disabilities community-based rehabilitation programs are required. In this regard, planning for and empowerment of individuals with physical disabilities can be more effective with six rehabilitational activities including family education, community education, individuals with physical disabilities referring to higher levels of support and guidance, providing rehabilitation aids, employment, and social support for individuals with physical disabilities. Keywords: City of Kashan, Individuals with Physical Disabilities, Leisure-Time, Structural Equation Modeling [1]. Associate Professor, Department of Geography and Ecotourism, Faculty of Natural     Resource and Earth Scince, University of Kashan. (Corresponding Author).     [email protected] [2]. Assistant Professor, Department of Geography and ecotourism, faculty of natural resource     and EarthScinceUniversity of Kashan.     [email protected] [3]. MA Student of Cultural Studies, Faculty of Natural Resource and EarthScinceUniversity     of Kashan
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