7 research outputs found
Nigella arvensis leaf extract mediated green synthesis of silver nanoparticles: Their characteristic properties and biological efficacy
No full textEnhanced hemocompatibility, antimicrobial and anti-inflammatory properties of biomolecules stabilized AgNPs with cytotoxic effects on cancer cells
No full textAbstract In the current research, we developed a safe method using Iranian yarrow extract for the synthesis of silver nanoparticles (IY-AgNPs) as reducing and stabilizing agents in different conditions. The prepared and stabilized IY-AgNPs under optimal conditions were characterized using FT-IR, XRD, TEM, and UV-vis techniques. Also, the blood-clotting, hemolytic, antioxidant, bactericidal and, fungicidal properties, cytotoxicity effects and inhibition of protein denaturation efficiency of IY-AgNPs were assessed in vitro. The stabilized IY-AgNPs with spherical shape and an average particle size of 19. 25 ± 7.9 nm did not show any hemolytic potential below 1000 µg/mL. These hemo-compatible NPs showed good blood-clotting ability by reducing clotting time (6 min relative to the control). These particles excellently inhibited the denaturation of bovine serum albumin (BSA) by 69.3–80.7% at concentrations ranging from 31.25 to 500 µg/mL compared to a reference drug. The outcomes showed that the IC50 values of IY-AgNPs were below 12.5 µg/mL against A375 cells and between 25 and 50 µg/mL against MCF-7 cancer cells. In addition, IY-AgNPs were bactericidal against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus (especially), and were fungicidal against Candida albicans. Biosynthesized IY-AgNPs indicated a significant antioxidant activity (63.2%) at a concentration of 350 µg/mL. These attained results suggested that bio/hemo-compatible IY-AgNPs may be a promising candidate for applications in the medicinal fields (particularly for wound healing) as anti-bleeding, antimicrobial, antioxidant, anti-inflammatory, and anticancer agents
Green approach for synthesis of gold nanoparticles from <i>Nigella arvensis</i> leaf extract and evaluation of their antibacterial, antioxidant, cytotoxicity and catalytic activities
No full text
Salicylic acid and jasmonic acid-mediated different fate of nickel phytoremediation in two populations of Alyssum inflatum Nyár.
No full textAbstract This study investigates Ni phytoremediation and accumulation potential in the presence of salicylic acid (SA) (0, 50 and 200 μM) and jasmonic acid (JA) (0, 5 and 10 μM) in two populations of Alyssum inflatum under various nickel (Ni) doses (0, 100 and 400 μM). By measuring Ni levels in the shoots and roots, values of bioaccumulation coefficient (BAC), biological concentration factor (BCF) and translocation factor (TF) were calculated to quantify Ni accumulation and translocation between plant organs. Additionally, the amounts of histidine (His), citric acid (CA) and malic acid (MA) were explored. The results showed that plant dry weight (DW) [in shoot (29.8%, 8.74%) and in root (21.6%, 24.4%)] and chlorophyll [a (17.1%, 32.5%), b (10.1%, 30.9%)] declined in M and NM populations respectively, when exposed to Ni (400 μM). Conversely, the levels of MA [in shoot (37.0%, 32.0%) and in root (25.5%, 21.2%)], CA [in shoot (17.0%, 10.0%) and in root (47.9%, 37.2%)] and His [in shoot (by 1.59- and 1.34-fold) and in root (by 1.24- and 1.18-fold)] increased. Also, in the presence 400 μM Ni, the highest accumulation of Ni was observed in shoots of M (1392 μg/g DW) and NM (1382 μg/g DW). However, the application of SA and JA (especially in Ni 400 μM + SA 200 μM + JA 5 and 10 μM treatments) mitigated the harmful impact of Ni on physiological parameters. Also, a decreasing trend was observed in the contents of MA, CA, and His. The reduction of these compounds as important chelators of Ni caused a decrease in root-to-shoot Ni transfer and reducing accumulation in the shoots of both populations. The values of phytoremediation indices in both populations exposed to Ni (400 μM) were above one. In presence of the SA and JA, these indices showed a decreasing trend, although the values remained above one (BAC, BCF and TF > 1). Overall, the results indicated that SA and JA can reduce phytoremediation potential of the two populations through different mechanisms
Salvigenin, a Trimethoxylated Flavone from Achillea Wilhelmsii C. Koch, Exerts Combined Lipid-Lowering and Mitochondrial Stimulatory Effects
No full textPhytochemical analysis of the Iranian plant Achillea wilhelmsii led to the isolation of 17 pure secondary metabolites belonging to the classes of sesquiterpenoids and phenolics. Two of these compounds, named wilhemsin (7) and wilhelmsolide (9), are new sesquiterpenoids, and the first shows undescribed structural features. Their structures were elucidated through extensive spectroscopic analysis, mainly based on 1D and 2D NMR, and chemical derivatization. Starting from plant traditional use and previous reports on the activity of the plant extracts, all the pure compounds were evaluated on endpoints related to the treatment of metabolic syndrome. The sesquiterpene hanphyllin (8) showed a selective cholesterol-lowering activity (−12.7% at 30 µM), santoflavone (13) stimulated glucose uptake via the GLUT transporter (+16.2% at 30 µM), while the trimethoxylated flavone salvigenin (14) showed a dual activity in decreasing lipid levels (−22.5% palmitic acid biosynthesis at 30 µM) and stimulating mitochondrial functionality (+15.4% at 30 µM). This study further confirms that, in addition to the antioxidants vitexin, isovitexin, and isoschaftoside, A. wilhelmsii extracts contain molecules that can act at different levels on the metabolic syndrome symptoms
