7 research outputs found
Games to familiarize children of 7-9 years old with the displacements of Badminton
No full textIn order that Badminton’s athlete manages to join the technical elements displacements are indispensable, that´s why this research shows the experience carried out to a group of 7-9 year-old children to familiarize them with the displacements in the sport Badminton, considering the difficulties they presented to dominate them. The purpose of the work focuses in proposing games that contribute to improve the assimilation of the displacements technique in Badminton teaching – learning process in these ages. To get the purpose theoretical and empiric methods were used, among the empirical ones the observation and the interview were used. In the content of the work some theoretical referents on the games and their use in the teaching of these sport basic technical skills are assumed, also it is given the physiologic and psychological characteristics of the group studied, as well as a group of 10 pre-sport games for the teaching of the displacements. The application of these games in the practice, starting from their structure, understanding and easy performance has allowed a better assimilation of the technical contents taught to the group studied
Pre-sport games of Badminton for the sport for all
No full textThe games are the fundamental activity in the children by means of which you/they are developed physical and intellectually, of here the motivation of investigating on the application of several of them in the sport for all, specifically with the project badminton. Keeping in mind the few games that are used to contribute to the development of the abilities in this sport, is that we think about to structure pre-sport games with that purpose and to improve this way the assimilation of the dexterities of the children that practice this discipline in the Sport for All". For it, besides the theoretical methods, empiric methods were used as the observation and the interview. The work shows the assimilation of the abilities and technical basic of the badminton, through games, jointly with aspects on the sport for all, besides being presented 8 structured games, which contribute to the learning of the basic abilities of the badminton
Serie de ejercicios para la técnica recibo activo de derecha y remate de los atletas de Tenis de Mesa de la categoría 11-12 años del sexo femenino de la EIDE “Ormani Arenado” de Pinar del Río
No full textCon el presente trabajo se muestra una propuesta de una serie de ejercicios con situaciones reales de juego para las técnicas recibo activo de derecha y remate en los atletas de Tenis de Mesa del sexo femenino de la categoría 11-12 años de la EIDE Ormani Arenado de Pinar de Río para el mejoramiento en la enseñanza, iniciación y desarrollo de la modalidad deportiva en cuestión, con ellos se puede aprender y/o ejercitar los aspectos técnico-tácticos necesarios para esta categoría, permitiendo una practica más eficiente. Tomamos estas técnicas porque las mismas tienen gran importancia dentro del accionar táctico por su importancia en la definición de las acciones. Para ello se tuvo en cuenta a los 6 atletas de esta categoría. Proponiendo como objetivo una serie de ejercicios para facilitar la ejecución de las técnicas recibo activo de derecha y remate. Para ello empleamos métodos teóricos y empíricos. Dentro de los teóricos el histórico lógico, inductivo deductivo, análisis síntesis, dentro de los empíricos trabajo con documentos, entrevistas, observación, valoración con especialistas. Nuestra propuesta reviste vital importancia porque con ella se logra confeccionar una serie de ejercicios para dichas técnicas que no existían con anterioridad en ningún documento, convirtiéndose en un arma imprescindible para los profesores/entrenadores de Tenis de Mesa de nuestro país en el momento de desarrollar los diferentes componentes de la preparación Técnico-Táctica
Diagnosis of Training for Badminton Coaches of Sports Initiation in Pinar del Río, Cuba
No full textEsta investigación propone posibles soluciones a las insuficiencias en la capacitación de los entrenadores de Bádminton de la provincia de Pinar del Río, las cuales quedaron evidenciadas tras un proceso de caracterización de su preparación y la definición de sus necesidades. Para ello se emplearon métodos teóricos y empíricos como el histórico - lógico, el análisis - síntesis, la inducción - deducción, la bservación, la entrevista y el análisis porcentual. A partir de los resultados arrojados por el diagnóstico, se determinaron las necesidades de capacitación de dichos entrenadores y se diseñó y ejecutó un programa de superación para dar respuesta a dichas necesidades, contribuyendo así a la superación de esta fuerza técnica con el propósito de alcanzar resultados superiores
El Tenis de Mesa una opción recreativa para los adolescentes entre 12 y 15 años, sexo masculino, zona # 33, Consejo Popular Sumidero
No full textEl conjunto de actividades deportivo-recreativa que se establece en esta investigación se van a poner en práctica en el Consejo popular Sumidero. Nos planteamos como objetivo Proponer actividades basadas en elementos del deporte Tenis de Mesa para la diversificación de las ofertas deportivo recreativas de los adolescentes entre 12 y 15 años del sexo masculino,zona # 33 del Consejo Popular Sumidero. Los elementos presentados permiten diversificar las ofertas para el disfrute de actividades de los adolescentes de una forma provechosa para ellos y para la Comunidad. Este trabajo que argumenta el carácter científico de las actividades Deportivorecreativas se estructura en dos capítulos, el primero permite conocer fundamentos teóricos sobre la actividad deportivo–recreativa comunitaria, la Comunidad de Sumidero y el Tenis de mesa como deporte y con fines recreativos. El segundo capítulo refiere los resultados de los instrumentos aplicados en el desarrollo de la investigación, las actividades presentadas y los avales otorgados por un número de especialistas tanto del deporte como de recreación. Teniendo en cuenta la importancia que tiene la realización de las actividades deportivo-recreativas en este medio es que nos decidimos a realizar esta investigación, que nos permitirá a partir de las actividades que proponemos con las características antes mencionadas, contribuir a la diversificación de las ofertas recreativas para los adolescentes del Consejo Popular
Determination of the bacterial microbiome of free-living amoebae isolated from wastewater by 16S rRNA amplicon-based sequencing
No full text[EN] Free-living amoebae (FLA) are ubiquitous protozoa commonly found in water. FLA are well-established hosts for amoeba-resistant bacteria, most of which are pathogenic, and offer them shelter from adverse environmental conditions or water treatments. Since there is very little knowledge about the complete bacterial microbiome of FLA, in this work the bacterial microbiome of FLA isolated from wastewater both after secondary and tertiary treatments was studied by amplicon-based sequencing. FLA were detected in 87.5% and 50.0% of wastewater samples taken after secondary and tertiary disinfection treatments, respectively. The most abundant bacterial phyla were Proteobacteria, Planctomycetes, Bacteroidetes and Firmicutes, which represented 83.77% of the total bacterial FLA microbiome. The most abundant class of bacteria was Gammaproteobacteria, which contains an important number of relevant pathogenic bacteria. The bacteria of public health concern Aeromonas, Arcobacter, Campylobacter, Helicobacter, Klebsiella, Legionella, Mycobacterium, Pseudomonas and Salmonella were detected as part of the FLA microbiome. Although different microbial communities were identified in each sample, there is no correlation between the microbiome of FLA and the extent of wastewater treatment. To our knowledge, this is the first work in which the bacterial microbiome of FLA isolated from wastewater is studied. Obtained results indicate that FLA are hosts of potentially pathogenic bacteria in treated wastewater used for irrigation, which may pose a public health threat.This work was supported by the Conselleria de Educación, Investigación, Cultura y Deporte, of the Community of Valencia, Spain, within the program of support for research under project AICO/2018/273.
The author Laura Moreno-Mesonero is the recipient of a technician contract funded by the Conselleria de Educación, Investigación, Cultura y Deporte, of the Community of Valencia, Spain, within the program of support for research under project AICO/2018/273.Moreno-Mesonero, L.; Ferrús Pérez, MA.; Moreno Trigos, MY. (2020). Determination of the bacterial microbiome of free-living amoebae isolated from wastewater by 16S rRNA amplicon-based sequencing. Environmental Research. 190:1-7. https://doi.org/10.1016/j.envres.2020.109987S17190Agustí, G., Codony, F., Fittipaldi, M., Adrados, B., & Morató, J. (2010). Viability Determination of Helicobacter pylori Using Propidium Monoazide Quantitative PCR. Helicobacter, 15(5), 473-476. doi:10.1111/j.1523-5378.2010.00794.xArslan, D., Legendre, M., Seltzer, V., Abergel, C., & Claverie, J.-M. (2011). Distant Mimivirus relative with a larger genome highlights the fundamental features of Megaviridae. Proceedings of the National Academy of Sciences, 108(42), 17486-17491. doi:10.1073/pnas.1110889108Balczun, C., & Scheid, P. (2017). Free-Living Amoebae as Hosts for and Vectors of Intracellular Microorganisms with Public Health Significance. Viruses, 9(4), 65. doi:10.3390/v9040065Batra, P., Mathur, P., & Misra, M. C. (2016). Aeromonas spp.: An Emerging Nosocomial Pathogen. Journal of Laboratory Physicians, 8(01), 001-004. doi:10.4103/0974-2727.176234Bertelli, C., Courtois, S., Rosikiewicz, M., Piriou, P., Aeby, S., Robert, S., … Greub, G. (2018). Reduced Chlorine in Drinking Water Distribution Systems Impacts Bacterial Biodiversity in Biofilms. Frontiers in Microbiology, 9. doi:10.3389/fmicb.2018.02520Bui, X. T., Winding, A., Qvortrup, K., Wolff, A., Bang, D. D., & Creuzenet, C. (2011). Survival of Campylobacter jejuni in co-culture with Acanthamoeba castellanii: role of amoeba-mediated depletion of dissolved oxygen. Environmental Microbiology, 14(8), 2034-2047. doi:10.1111/j.1462-2920.2011.02655.xCaporaso, J. G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F. D., Costello, E. K., … Knight, R. (2010). QIIME allows analysis of high-throughput community sequencing data. Nature Methods, 7(5), 335-336. doi:10.1038/nmeth.f.303Chouari, R., Le Paslier, D., Daegelen, P., Ginestet, P., Weissenbach, J., & Sghir, A. (2003). Molecular Evidence for Novel Planctomycete Diversityin a Municipal Wastewater TreatmentPlant. Applied and Environmental Microbiology, 69(12), 7354-7363. doi:10.1128/aem.69.12.7354-7363.2003Comeau, A. M., Douglas, G. M., & Langille, M. G. I. (2017). Microbiome Helper: a Custom and Streamlined Workflow for Microbiome Research. mSystems, 2(1). doi:10.1128/msystems.00127-16Delafont, V., Brouke, A., Bouchon, D., Moulin, L., & Héchard, Y. (2013). Microbiome of free-living amoebae isolated from drinking water. Water Research, 47(19), 6958-6965. doi:10.1016/j.watres.2013.07.047García, A., Goñi, P., Clavel, A., Lobez, S., Fernandez, M. T., & Ormad, M. P. (2011). Potentially pathogenic free-living amoebae (FLA) isolated in Spanish wastewater treatment plants. Environmental Microbiology Reports, 3(5), 622-626. doi:10.1111/j.1758-2229.2011.00271.xGómez-Couso, H., Paniagua-Crespo, E., & Ares-Mazás, E. (2006). Acanthamoeba as a temporal vehicle of Cryptosporidium. Parasitology Research, 100(5), 1151-1154. doi:10.1007/s00436-006-0377-7Greub, G., & Raoult, D. (2004). Microorganisms Resistant to Free-Living Amoebae. Clinical Microbiology Reviews, 17(2), 413-433. doi:10.1128/cmr.17.2.413-433.2004Huse, S. M., Dethlefsen, L., Huber, J. A., Mark Welch, D., Relman, D. A., & Sogin, M. L. (2008). Correction: Exploring Microbial Diversity and Taxonomy Using SSU rRNA Hypervariable Tag Sequencing. PLoS Genetics, 4(12). doi:10.1371/annotation/3d8a6578-ce56-45aa-bc71-05078355b851Klindworth, A., Pruesse, E., Schweer, T., Peplies, J., Quast, C., Horn, M., & Glöckner, F. O. (2012). Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Research, 41(1), e1-e1. doi:10.1093/nar/gks808Kopylova, E., Noé, L., & Touzet, H. (2012). SortMeRNA: fast and accurate filtering of ribosomal RNAs in metatranscriptomic data. Bioinformatics, 28(24), 3211-3217. doi:10.1093/bioinformatics/bts611Li, Q., Yu, S., Li, L., Liu, G., Gu, Z., Liu, M., … Ren, L. (2017). Microbial Communities Shaped by Treatment Processes in a Drinking Water Treatment Plant and Their Contribution and Threat to Drinking Water Safety. Frontiers in Microbiology, 8. doi:10.3389/fmicb.2017.02465Lyczak, J. B., Cannon, C. L., & Pier, G. B. (2000). Establishment of Pseudomonas aeruginosa infection: lessons from a versatile opportunist1*Address for correspondence: Channing Laboratory, 181 Longwood Avenue, Boston, MA 02115, USA. Microbes and Infection, 2(9), 1051-1060. doi:10.1016/s1286-4579(00)01259-4Magnet, A., Fenoy, S., Galván, A. L., Izquierdo, F., Rueda, C., Fernandez Vadillo, C., & del Aguila, C. (2013). A year long study of the presence of free living amoeba in Spain. Water Research, 47(19), 6966-6972. doi:10.1016/j.watres.2013.09.065Marciano-Cabral, F., & Cabral, G. (2003). Acanthamoeba
spp. as Agents of Disease in Humans. Clinical Microbiology Reviews, 16(2), 273-307. doi:10.1128/cmr.16.2.273-307.2003Marshall, B. (2002). Helicobacter pylori: 20 years on. Clinical Medicine, 2(2), 147-152. doi:10.7861/clinmedicine.2-2-147Maschio, V. J., Chies, F., Carlesso, A. M., Carvalho, A., Rosa, S. P., Van Der Sand, S. T., & Rott, M. B. (2014). Acanthamoeba T4, T5 and T11 Isolated From Mineral Water Bottles in Southern Brazil. Current Microbiology, 70(1), 6-9. doi:10.1007/s00284-014-0676-7José Maschio, V., Corção, G., & Rott, M. B. (2015). IDENTIFICATION OF Pseudomonas spp. AS AMOEBA-RESISTANT MICROORGANISMS IN ISOLATES OF Acanthamoeba. Revista do Instituto de Medicina Tropical de São Paulo, 57(1), 81-83. doi:10.1590/s0036-46652015000100012Montalbano Di Filippo, M., Santoro, M., Lovreglio, P., Monno, R., Capolongo, C., Calia, C., … Di Cave, D. (2015). Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy. International Journal of Environmental Research and Public Health, 12(4), 3417-3427. doi:10.3390/ijerph120403417Moreno, Y., Moreno-Mesonero, L., & García-Hernández, J. (2019). DVC-FISH to identify potentially pathogenic Legionella inside free-living amoebae from water sources. Environmental Research, 176, 108521. doi:10.1016/j.envres.2019.06.002Moreno-Mesonero, L., Hortelano, I., Moreno, Y., & Ferrús, M. A. (2020). Evidence of viable Helicobacter pylori and other bacteria of public health interest associated with free-living amoebae in lettuce samples by next generation sequencing and other molecular techniques. International Journal of Food Microbiology, 318, 108477. doi:10.1016/j.ijfoodmicro.2019.108477Moreno-Mesonero, L., Moreno, Y., Alonso, J. L., & Ferrús, M. A. (2016). DVC-FISH and PMA-qPCR techniques to assess the survival of Helicobacter pylori inside Acanthamoeba castellanii. Research in Microbiology, 167(1), 29-34. doi:10.1016/j.resmic.2015.08.002Reyes-Batlle, M., Wagner, C., López-Arencibia, A., Sifaoui, I., Martínez-Carretero, E., Valladares, B., … Lorenzo-Morales, J. (2017). Isolation and molecular characterization of a Naegleria strain from a recreational water fountain in Tenerife, Canary Islands, Spain. Acta Parasitologica, 62(2). doi:10.1515/ap-2017-0033Rodríguez-Zaragoza, S. (1994). Ecology of Free-Living Amoebae. Critical Reviews in Microbiology, 20(3), 225-241. doi:10.3109/10408419409114556Rognes, T., Flouri, T., Nichols, B., Quince, C., & Mahé, F. (2016). VSEARCH: a versatile open source tool for metagenomics. PeerJ, 4, e2584. doi:10.7717/peerj.2584Rowbotham, T. J. (1980). Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. Journal of Clinical Pathology, 33(12), 1179-1183. doi:10.1136/jcp.33.12.1179Scheikl, U., Sommer, R., Kirschner, A., Rameder, A., Schrammel, B., Zweimüller, I., … Walochnik, J. (2014). Free-living amoebae (FLA) co-occurring with legionellae in industrial waters. European Journal of Protistology, 50(4), 422-429. doi:10.1016/j.ejop.2014.04.002Siddiqui, R., & Khan, N. A. (2012). War of the microbial worlds: Who is the beneficiary in Acanthamoeba–bacterial interactions? Experimental Parasitology, 130(4), 311-313. doi:10.1016/j.exppara.2012.01.021Steenbergen, J. N., Shuman, H. A., & Casadevall, A. (2001). Cryptococcus neoformans interactions with amoebae suggest an explanation for its virulence and intracellular pathogenic strategy in macrophages. Proceedings of the National Academy of Sciences, 98(26), 15245-15250. doi:10.1073/pnas.261418798Tezcan-Merdol, D., Ljungström, M., Winiecka-Krusnell, J., Linder, E., Engstrand, L., & Rhen, M. (2004). Uptake and Replication of
Salmonella enterica
in
Acanthamoeba rhysodes. Applied and Environmental Microbiology, 70(6), 3706-3714. doi:10.1128/aem.70.6.3706-3714.2004Thomas, J. M., & Ashbolt, N. J. (2010). Do Free-Living Amoebae in Treated Drinking Water Systems Present an Emerging Health Risk? Environmental Science & Technology, 45(3), 860-869. doi:10.1021/es102876yThomas, V., McDonnell, G., Denyer, S. P., & Maillard, J.-Y. (2010). Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality. FEMS Microbiology Reviews, 34(3), 231-259. doi:10.1111/j.1574-6976.2009.00190.xValster, R. M., Wullings, B. A., Bakker, G., Smidt, H., & van der Kooij, D. (2009). Free-Living Protozoa in Two Unchlorinated Drinking Water Supplies, Identified by Phylogenic Analysis of 18S rRNA Gene Sequences. Applied and Environmental Microbiology, 75(14), 4736-4746. doi:10.1128/aem.02629-08White, C.I., Birtles, R.J., Wigley, P., Jones, P.H., Mycobacterium avium subspecies paratuberculosis in free-living amoebae isolated from fields not used for grazing. Vet. Rec., 166: 401-402. https://doi.org/10.1136/vr.b4797.Young, K. T., Davis, L. M., & DiRita, V. J. (2007). Campylobacter jejuni: molecular biology and pathogenesis. Nature Reviews Microbiology, 5(9), 665-679. doi:10.1038/nrmicro1718Yousuf, F. A., Siddiqui, R., & Khan, N. A. (2013). Acanthamoeba castellanii of the T4 genotype is a potential environmental host for Enterobacter aerogenes and Aeromonas hydrophila. Parasites & Vectors, 6(1). doi:10.1186/1756-3305-6-169Zhang, J., Kobert, K., Flouri, T., & Stamatakis, A. (2013). PEAR: a fast and accurate Illumina Paired-End reAd mergeR. Bioinformatics, 30(5), 614-620. doi:10.1093/bioinformatics/btt593Zhang, T., Shao, M.-F., & Ye, L. (2011). 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants. The ISME Journal, 6(6), 1137-1147. doi:10.1038/ismej.2011.18
Tonsillitis and sore throat in children
No full textSurgery of the tonsils is still one of the most frequent procedures during childhood. Due to a series of fatal outcomes after hemorrhage in children in Austria in 2006, the standards and indications for tonsillectomy have slowly changed in Germany. However, no national guidelines exist and the frequency of tonsil surgery varies across the country. In some districts eight times more children were tonsillectomized than in others. A tonsillectomy in children under six years should only be done if the child suffers from recurrent acute bacterially tonsillitis. In all other cases (i.e. hyperplasia of the tonsils) the low risk partial tonsillectomy should be the first line therapy. Postoperative pain and the risk of hemorrhage are much lower in partial tonsillectomy (=tonsillotomy). No matter whether the tonsillotomy is done by laser, radiofrequency, shaver, coblation, bipolar scissor or Colorado needle, as long as the crypts are kept open and some tonsil tissue is left behind. Total extracapsular tonsillectomy is still indicated in severely affected children with recurrent infections of the tonsils, allergy to antibiotics, PFAPA syndrome (periodic fever, aphthous stomatitis, pharyngitis, and cervical adenitis) and peritonsillar abscess. With regard to the frequency and seriousness of the recurrent tonsillitis the indication for tonsillectomy in children is justified if 7 or more well-documented, clinically important, adequately treated episodes of throat infection occur in the preceding year, or 5 or more of such episodes occur in each of the 2 preceding years (according to the paradise criteria). Diagnosis of acute tonsillitis is clinical, but sometimes it is hard to distinguish viral from bacterial infections. Rapid antigen testing has a very low sensitivity in the diagnosis of bacterial tonsillitis and swabs are highly sensitive but take a long time. In all microbiological tests the treating physician has to keep in mind, that most of the bacterials, viruses and fungi belong to the healthy flora and do no harm. Ten percent of healthy children even bear strepptococcus pyogenes all the time in the tonsils with no clinical signs. In these children decolonization is not necessary. Therefore, microbiological screening tests in children without symptoms are senseless and do not justify an antibiotic treatment (which is sometimes postulated by the kindergartens). The acute tonsillitis should be treated with steroids (e.g. dexamethasone), NSAIDs (e.g. ibuprofene) and betalactam antibiotics (e.g. penicillin or cefuroxime). With respect to the symptom reduction and primary healing the short-term late-generation antibiotic therapy (azithromycin, clarithromycin or cephalosporine for three to five days) is comparable to the long-term penicilline therapy. There is no difference in the course of healing, recurrence or microbiological resistance between the short-term penicilline therapy and the standard ten days therapy. On the other hand, only the ten days antibiotic therapy has proven to be effective in the prevention of rheumatic fever and glomerulonephritic diseases. The incidence of rheumatic heart disease is currently 0.5 per 100,000 children of school age. The main morbidity after tonsillectomy is pain and the late haemorrhage. Posttonsillectomy bleeding can occur till the whole wound is completely healed, which is normally after three weeks. Life-threatening haemorrhages occur often after smaller bleedings, which can spontaneously cease. That is why every haemorrhage, even the smallest, has to be treated properly and in ward. Patients and parents have to be informed about the correct behaviour in case of haemorrhage with a written consent before the surgery.The handout should contain important addresses, phone numbers and contact persons. Almost all cases of fatal outcome after tonsillectomy were due to false management of haemorrhage. Haemorrhage in small children can be especially life-threatening because of the lower blood volume and the danger of aspiration with asphyxia. A massive haemorrhage is an extreme challenge for every paramedic or emergency doctor because of the difficult airway management. Intubation is only possible with appropriate inflexible suction tubes. All different surgical techniques have the risk of haemorrhage and even the best surgeon will experience a postoperative haemorrhage. The lowest risk of haemorrhage is after cold dissection with ligature or suturing. All "hot" techniques with laser, radiofrequency, coblation, mono- or bipolar forceps have a higher risk of late haemorrhage. Children with a hereditary coagulopathy have a higher risk of haemorrhage. It is possible, that these children were not identified before surgery. Therefore it is recommended by the Society of paediatrics, anaesthesia and ENT, that a standardised questionnaire should be answered by the parents before tonsillectomy and adenoidectomy. This 17-point-checklist questionnaire is more sensitive and easier to perform than a screening with blood tests (e.g. INR and PTT). Unfortunately, a lot of surgeons still screen the children preoperatively by coagulative blood tests, although these tests are inappropriate and incapable of detecting the von Willebrand disease, which is the most frequent coagulopathy in Europe. The preoperative information about the surgery should be done with the child and the parents in a calm and objective atmosphere with a written consent. A copy of the consent with the signature of the surgeon and both custodial parents has to be handed out to the parents
