25 research outputs found
Hybrid lunar inflatable structure
No full textBefore man had even stepped foot on the lunar surface, the idea of establishing a base was discussed extensively, be it in science fiction or academia. Although, to this day there has not been an actual base on the Moon, many studies have been conducted regarding design possibilities and environmental impacts. There exists a prevalent challenge in regards to the predictability of the effects the lunar environment has on surface structures. This thesis discusses those aspects of lunar habitats and proposes a new design concept.M.S.Includes bibliographical referencesby Rohith Dronadul
Synthesis of Sensitive Oligodeoxynucleotides Containing Acylated Cytosine, Adenine, and Guanine Nucleobases
No full textBackground/Objective: Oligodeoxynucleotides (ODNs) containing base-labile modifications such as N4-acetyldeoxycytidine (4acC), N6-acetyladenosine (6acA), N2-acetylguanosine (2acG), and N4-methyoxycarbonyldeoxycytidine (4mcC) are highly challenging to synthesize because standard ODN synthesis methods require deprotection and cleavage under strongly basic and nucleophilic conditions, and there is a lack of ideal alternative methods to solve the problem. The objective of this work is to explore the capability of the recently developed 1,3-dithian-2-yl-methoxycarbonyl (Dmoc) method for the incorporation of multiple 4acC modifications into a single ODN molecule and the feasibility of using the method for the incorporation of the 6acA, 2acG and 4mcC modifications into ODNs. Methods: The sensitive ODNs were synthesized on an automated solid phase synthesizer using the Dmoc group as the linker and the methyl Dmoc (meDmoc) group for the protection of the exo-amino groups of nucleobases. Deprotection and cleavage were achieved under non-nucleophilic and weakly basic conditions. Results: The 4acC, 6acA, 2acG, and 4mcC were all found to be stable under the mild ODN deprotection and cleavage conditions. Up to four 4acC modifications were able to be incorporated into a single 19-mer ODN molecule. ODNs containing the 6acA, 2acG, and 4mcC modifications were also successfully synthesized. The ODNs were characterized using RP HPLC, capillary electrophoresis, gel electrophoresis and MALDI MS. Conclusions: Among the modified nucleotides, 4acC has been found in nature and proven beneficial to DNA duplex stability. A method for the synthesis of ODNs containing multiple 4acC modifications is expected to find applications in biological studies involving 4acC. Although 6acA, 2acG, and 4mcC have not been found in nature, a synthetic route to ODNs containing them is expected to facilitate projects aimed at studying their biophysical properties as well as their potential for antisense, RNAi, CRISPR, and mRNA therapeutic applications
Dependence of equilibrium stacking fault width on thickness of Cu thin films: A molecular dynamics study
No full textApplications of Fixator-norator Pair in Companion Model Based Designs
No full textAbstractThis paper presents a novel approach to the accurate time domain analysis and design of analog circuits containing storage elements by making use of ‘companion models’. The emphasis is given to the design using fixator-norator pair, which is now ingenious in analog circuit design. The Backward Euler method can render a storage element to an equivalent circuit, containing only linear components viz. current source or voltage source and resistance. This distinctive feature allows us to generate equivalent companion models of the storage elements for every time step, whereby persuading the application of fixator-norator pairs in such circuits containing reactive components. This paper briefly explains the construction of equivalent circuits at desired time steps and provides a detailed explanation on the design of the gain of an op-amp differentiator for a given input by exploiting the fixator-norator method. The proposed method reveals to be much reliable and accurate in the design of circuits containing reactive elements
Mid infrared spectroscopic imaging and tomography
No full textFourier Transform Infrared (FT-IR) Spectroscopic Imaging is an optical technique that simultaneously provides morphological (two dimensional) and mid-infrared spectral (one dimensional) information for a specimen under observation. In this dissertation, we develop a new instrument that extends the capabilities of FT-IR imaging to provide three dimensional morphological information along with mid-infrared spectral information on the microscopic length scale. We first demonstrate that spectroscopic information from the mid-infrared can be used to perform histologic recognition and cancer detection in prostate, breast and skin cancer tissues. These studies utilize FT-IR imaging data of thin tissue slices from several hundred patients. The segmentation algorithms and instrumentation used are described in detail. We perform a rigorous analysis of instrumentation and data so as to understand the limits and limitations of current state-of-the-art instrumentation. We then propose a new instrument that adds a new dimension of information to current instruments and establish the new state-of-the-art in mid-infrared imaging. The theoretical basis of such instrumentation is presented in detail and techniques for visualization of four dimensional data are outlined.Item withdrawn by Alexis Thompson ([email protected]) on 2013-02-21T21:53:07Z
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University of Illinois Theses & Dissertations (ID: 1)
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Synthesis of Sensitive RNAs Using Fluoride-Cleavable Groups as Linkers and Amino-Group Protection
No full textA chemical method suitable for the synthesis of RNAs containing modifications such as N4-acetylcytidine (ac4C) that are unstable under the basic and nucleophilic conditions used by standard RNA synthesis methods is described. The method uses the 4-((t-butyldimethylsilyl)oxy)-2-methoxybutanoyl (SoM) group for the protection of exo-amino groups of nucleobases and the 4-((t-butyldimethylsilyl)oxy)-2-((aminophosphaneyl)oxy)butanoyl (SoA) group as the linker for solid phase synthesis. RNA cleavage and amino deprotection are achieved using fluoride under the same conditions used for the removal of the 2\u27-OH silyl protecting groups. Using this method, a wide range of electrophilic and base-sensitive groups including those that play structural and regulatory roles in biological systems and those that are artificially designed for various purposes are expected to be able to be incorporated into any position of any RNA sequences. As a proof of concept, several RNAs containing the highly sensitive ac4C epitranscriptomic modification was synthesized and purified with RP HPLC. MALDI MS analysis indicated that the ac4C modification is completely stable under the fluoride deprotection conditions. The sensitive RNA synthesis method is expected to be able to overcome the long-lasting obstacle of accessing various modified sensitive RNAs to projects in areas such as epitranscriptomics, molecular biology and the development of nucleic acid therapeutics
In vitro studies of graphene oxide reinforced hydroxyapatite nanobiocomposite on human erythrocytes
No full textGrowth and characterization of divalent transition metal ions doped zinc hydrogen phosphate single crystals
No full textRetracted: When a giant ovarian cyst poses a diagnostic dilemma
No full textThe article " When a giant ovarian cyst poses a diagnostic dilemma" is retracted by the Editor-in-Chief, on the request of corresponding author and co-authors. The corresponding author informed that the patient described in this article, although willingly gave her consent for revealing her clinical data for publication, later withdrew her consent after knowing about the publication of clinical material during she came for a follow up visit
