1,179 research outputs found
Study of Morphokinetics in Day 3 Embryo with Implantation Potential and Effect of Sperm Cryopreservation on Embryogenesis
No full textABSTRACTAimIn recent past, many studies had come up with the combination of time-lapse (TL) imaging of embryo morphokinetics as a noninvasive means for improving embryo selection andin vitrofertilization (IVF) success. The primary objective of the study was to find out if there is significant variation in morphokinetics of embryos with different implantation potential and also to study the effect of sperm freezing on time points of embryogenesis events in embryos with implantation potential.Materials and methodsKinetic data and cycle outcomes were analyzed retrospectively in 142 patients who had undergone IVF/intracytoplasmic sperm injection (ICSI) cycles using semen with normal parameters and embryo transfer (ET) on day 3. For the surety of specificity of morphokinetics, only cases with single ET cycles were included in the study. Timing of specific events, from the point of ICSI, was determined using TL imaging. Kinetic markers like time to syngamy (t-pnf), t2, time to two cells (c), 3c (t3), 4c (t4), 5c (t5), 8c (t8), tMor, CC2, CC3, t5–t2, t5–t4, s1, s2, and s3 were calculated. The cleavage synchronicity from the 2–8 cell stage (CS2–8), from 4 to 8 cell stage (CS4–8), and from 2 to 4 cell stage (CS2–4) were calculated as defined elsewhere. Deoxyribonucleic acid replication time ratio (DR) was also included in the comparison. Analysis of variance test was used for comparison of the mean timing of cell division and cell cycle intervals.ResultsMorphokinetics t-pnf, t2, t8, CC2, S2, S3, CS2–8, CS4–8, and CS2–4 differed significantly between embryos with and without implantation potential, when embryos were developed using fresh semen, while t3, t4, t5, CC2, S2, t5–t2, CS2–4, and DR differed significantly between the embryos with and without implantation potential when frozen semen was used. No significant difference was found in mean value of any of the above-stated parameters when comparison was done between implanted embryos fertilized by either fresh or cryopreserved sperm.ConclusionMany morphokinetics parameters of embryogenesis vary significantly between embryos with different ability to implant; therefore, the criteria developed in our IVF lab can be useful for selection of suitable embryo even at day 3 of development with more chances of implantation.Clinical significanceStudy indicates necessity of development of individualized selection model based on morphokinetics for every IVF lab and also confirms freezing as an important tool for fertility preservation of males as it does not affect events of embryogenesis.How to cite this articleBhadarka HK, Patel NH, Patel KB, Sodagar NR, Jadeja YD, Patel NH, Patel MN, Patel AV, Patel DH, Patel JS. Study of Morphokinetics in Day 3 Embryo with Implantation Potential and Effect of Sperm Cryopreservation on Embryogenesis. Int J Infertil Fetal Med 2017;8(2):61-67.</jats:sec
Direct Production of Graphene Nanosheets for Near Infrared Photoacoustic Imaging.
No full textHummers method is commonly used for the fabrication of graphene oxide (GO) from graphite particles. The oxidation process also leads to the cutting of graphene sheets into small pieces. From a thermodynamic perspective, it seems improbable that the aggressive, somewhat random oxidative cutting process could directly result in graphene nanosheets without destroying the intrinsic π-conjugated structures and the associated exotic properties of graphene. In Hummers method, both KMnO4 and NO2þ (nitronium ions) in concentrated H2SO4 solutions act as oxidants via different oxidation mechanisms. From both experimental observations and theoretical calculations, it appears that KMnO4 plays a major role in the observed oxidative cutting and unzipping processes. We find that KMnO4 also limits nitronium oxidative etching of graphene basal planes, therefore slowing down graphene fracturing processes for nanosheet fabrication. By intentionally excluding KMnO4 and exploiting pure nitronium ion oxidation, aided by the unique thermal and kinetic effects induced by microwave heating, we find that graphite particles can be converted into graphene nanosheets with their π-conjugated aromatic structures and properties largely retained. Without the need of any postreduction processes to remove the high concentration of oxygenated groups that results from Hummers GO formation, the graphene nanosheets as-fabricated exhibit strong absorption, which is nearly wavelength-independent in the visible and near-infrared (NIR) regions, an optical property typical for intrinsic graphene sheets. For the first time, we demonstrate that strong photoacoustic signals can be generated from these graphene nanosheets with NIR excitation. The photo-to-acoustic conversion is weakly dependent on the wavelength of the NIR excitation, which is different from all other NIR photoacoustic contrast agents previously reported.This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in ACS Nano, copyright © American Chemical Society after peer review. To access the final edited and published work see https://dx.doi.org/10.1021/nn403429v
Reading at risk: why effective literacy practice is not effective.
No full textThe gap between high and low achievers in reading is wide in New Zealand compared to other countries as shown in PIRLS (Progress in International Reading Literacy Study) 2001 and 2006 studies. Students of minority backgrounds and low socio-economic status are over-represented in the low achieving category. As the primary response to reduce the achievement gap, the Government developed and distributed the literacy teaching reference Effective Literacy Practice in Years 1 to 4. This article examines Effective Literacy Practice against current scientificbased international research in the teaching of reading, with particular emphasis on the teaching of reading to students at risk. Research evidence shows that an explicit and systematic approach to teaching reading is critical to the success of reading achievement with at-risk students. With a heavy leaning towards incidental learning, Effective Literacy Practice fails to provide teachers with the necessary knowledge to effectively teach the critical components of reading
Psychological, social and welfare interventions for psychological health and well-being of torture survivors
No full textThis is the protocol for a review and there is no abstract. The objectives are as follows: Primary objective 1. To assess beneficial and adverse effects of psychological, social and welfare interventions versus no treatment for the reduction of psychological distress in torture survivors. Secondary objectives 2. To describe the quality and generalisability of the studies evaluating the effects of these treatment approaches on torture survivors, and specifically: • to provide an objective assessment of risk of bias in these studies; • to describe the specific populations evaluated in studies of torture survivors (including demographics, torture experiences and psychological status); • to describe the variety of interventions that have been evaluated in these populations; and • to describe the outcomes evaluated in these intervention studies
Synthesis of novel biologically active methylene derivatives of sydnones
No full textA series of mono and bis N-Mannich bases incorporating 3-(4-methylphenyl)- 4-(substituted-1/4-ylmethyl)sydnone scaffolds were synthesized and tested for their antibacterial, antifungal, anticancer, and antiviral activities. Most of the compounds showed moderate-to-significant antibacterial and antifungal activities. The compounds did not show selective activity against HIV. Some of the compounds have been evaluated for anticancer activity, but they were found as poorly active. The structures of the synthesized compounds were elucidated by IR and NMR spectroscopy. © 2013 Springer Science+Business Media New York.sponsorship: One of the author (STA) would like to express his gratitude to the National Cancer Institute, Bethesda, MD, USA for anticancer activity data. He also wants to thank the University Grants Commission, New Delhi for providing a Research Fellowship. He is also grateful to Atul Limited, Valsad and Ami Organics, Sachin for providing useful chemicals. (University Grants Commission, New Delhi)status: Publishe
Successful Treatment of Long-Term, Poststroke, Upper-Limb Spasticity With OnabotulinumtoxinA
No full textBench-to-bedside review: Erythropoietin and its derivatives as therapies in critical care
No full textAuthor can archive publisher's pdf. Free via Creative Commons: CC-BENCHTOBEDSIDE-2.0. © 2012 BioMed Central Ltd
Tirmilyra concinnus Patel & Naderloo & Trivedi & Mitra 2023, n. comb.
No full text<i>Tirmilyra concinnus</i> (Ghani & Tirmizi, 1995) n. comb. <p>(Figs. 1E, F, 2C, D, 3C, D, 4E, F)</p> <p> <i>Philyra concinnus</i> Ghani & Tirmizi, 1995: 144–147, fig 1a–h (material from Karachi).— Apel, 2001: 56; Ng <i>et al</i>. 2008: 92 (in list); Galil 2009: 281, tab 1; Naderloo & Türkay 2012: 32; Naderloo 2017: 109, figs 14.35c, 14.37, 14.38.</p> <p> <i>Philyra sexangula —</i> Stephensen, 1946: 89.</p> <p> <i>Ebalia sagittifera —</i> Cooper, 1997: 173, 174 (partly), fig 7.</p> <p> <b>Material examined.</b> ZUTC 6925, 1 male (CL 8.4 mm, CW 7.6 mm), 2 females (CL 9.3 mm, CW 8.2 mm; CL 9.4 mm, CW 8.4 mm), Hormoz Island, Persian Gulf, unknown collector and date; ZUTC 1341, 1 male, 1 female, coast of Bushehr, Persian Gulf, 28°58′N, 50°49′E, sandy/rocky, 10.2006, coll. R. Naderloo; ZUTC 1342, 2 males, 1 female, Qeshm I., Persian Gulf, coll. M. Asgari; ZUTC 1343, 1 male, 1 female, Badnar Kolahi, Strait of Hormoz, 27°02′N, 56°51′E, muddy-sand flat, 22.04.2008, coll. R. Naderloo; ZUTC 1344, 1 juvenile, East of city, Bandar-Abbas, Persian Gulf, 27°11′N, 56°21′E, muddy-sand flat with shells, 23.04.2008, coll. R. Naderloo, A. Kazemi, A. Keykhosravi; ZUTC 1345, 1 male, Jofreh, Bushehr, Persian Gulf, 28°58′N, 50°49′E, sandy/rocky, dead coral with algae, 24.05.2008, coll. R. Naderloo, A. Kazemi, H. Salehi.</p> <p> <b>Description.</b> Carapace hexagonal (Fig. 1E), slightly longer than broad. Dorsal surface smooth except three narrow minutely granular ridges uniting to form "broad-arrow" pointing forwards, middle ridge extendingfrom progastric to intestinal region; lateral ridges extending backwards laterally to branchial region, running parallel to anterolateral margins, with ends projecting beyond posterolateral margins, forming larger denticles (Fig. 1E); regions relatively distinct, with elevated gastric, cardiac, branchial, intestinal regions; hepatic region excavated forming depression, upper and lower margins beaded, originating from middle of anterolateral margin, not merging anteriorly, broadly triangular tooth projecting on anterior half of lower margin of anterolateral border; anterolateral margin convex, shorter than posterolateral margin, merging with posterolateral margin to form broad triangular tooth; anterolateral, posterolateral, posterior margins beaded; epibranchial angle obtuse; posterolateral margin slightly sinuous; front smooth, almost straight, epistome visible beyond frontal margin from dorsal view; posterior margin almost straight, slightly convex, lateral sides with strong, pointed, outwardly directed spines, spines or small obtuse projections (Fig. 1E). Anterior margin of endostome reaching slightly beyond anterior margin of inhalent channels.</p> <p>Maxilliped 3 (Fig. 3C) smooth, entirely covering endostome; merus with notch, two-thirds as long as ischium along inner margin; ischium two times longer than wide; carpus not visible in external view when reposed; propodus and dactylus visible in external view, articulating subdistally on inner surface of merus; exopod (Fig. 3C) outer margin convex, setose, much longer than wide, almost twice the length of merus.</p> <p>Chelipeds (Fig. 1E, F) equal, as long as carapace length, granulated. Merus triquetral in cross-section with edges raised and granular; both inner and outer margins with tuberculated ridge. Carpus and propodus have raised row of granules along inner edge of their upper surface. Fingers slender, about two-thirds length of propodus, cutting edge of fingers toothed with scattered setae, median shallow canal extending to distal length of both fingers.</p> <p>P2–P5 (Fig. 1E) subcylindrical; total length of all pairs almost similar, merus and carpus glabrous, merus longest as compared to carpus, propodus and dactylus. Carpus and propodus almost equal in length, dactylus large with tapering distal end.</p> <p>Thoracic sternum (Fig. 1F) smooth, tuberculated on margins, slightly concave. Sternites 1–3 completely fused without any trace of sutures, sternite 3 separated from sternite 4 by shallow groove; sternite 4–6 almost similar in width, sternite 7 slightly tuberculated.</p> <p>Male pleon (Fig. 1F, 3D) smooth with three articulating parts: somite 1 free, narrow; somite 2–6 fused, immovable, posterolateral angles with small bulge, somite 6 without any denticle; telson triangular, longer than broad with curved apex.</p> <p>Female pleon ovate; somite 1 free; somites 2–6 fused, suture clearly visible between somites 2 and 3, partial suture visible between somites 3–4 and 5–6, forming domed plate almost completely covering thoracic sternum. Telson triangular, longer than broad, with curved apex (Fig. 2D).</p> <p>G1 (Fig. 4E, F) shaft long, slender, straight, apical process long, about 0.2 times as long as whole length, more sinuous-shape, with spade-shape (spatulate) ending, apical lobe bearing setae almost length of the tip. Female gonopore (Fig. 5D) on inner anterior edge of sternite 5, oval in shape with broad opening.</p> <p> <b>Variation</b>. The carapace of female resembles with that of male, except for lateral sides of posterior margin having sharp dorsoventrally flattened teeth (Fig. 2C).</p> <p> <b>Distribution.</b> Pakistan (Ghani & Tirmizi 1995), Persian Gulf [Stephensen (1946) as <i>Philyra sexangula</i>; Apel 2001; Naderloo & Türkay 2012; Naderloo 2017] and Gulf of Oman (Naderloo <i>et al</i>. 2015).</p> <p> <b>Remarks.</b> Ghani & Tirmizi (1995) described <i>T. concinnus</i> <b>n. comb.</b> on the basis of specimens collected from Karachi (Pakistan) and Persian Gulf. As mentioned earlier, the Persian Gulf material examined by these authors is referable to <i>T. sagittifera</i> <b>n. comb.</b> (Apel 2001; Naderloo & Türkay 2012; Naderloo 2017). Here we also confirm that the drawing of a Persian Gulf specimen by Ghani & Tirmizi (1995: fig. 2) agrees with type specimen of <i>P. sagittifera</i> in the following features: carapace hexagonal, smooth except three broad tuberculated ridges unite to form a "broad-arrow" point; epibranchial angle forming right angle, posterior margin convex, lateral sides with knob-like teeth having rounded tips, small median tooth present on the posterior margin in males; and G1 shaft straight, slender, with apical process slightly sinuous-shape, with tapering end.</p> <p> The holotype and some of the paratypes of <i>T. concinnus</i> <b>n. comb.</b> deposited in the Marine Reference Collection and Resource Centre, University of Karachi, Karachi, Pakistan are not traceable despite many searches in the museum (Noor Saher, personal communication). The second author has examined the paratype specimens (1 male and 1 female) deposited in Naturalis Biodiversity Center, Leiden, but these are in very poor condition and of little use for identification. The fresh specimens from the Persian Gulf examined here are in agreement with the description and illustrations of holotype provided by Ghani & Tirmizi (1995).</p> <p> <i>Tirmilyra concinnus</i> <b>n. comb.</b> closely resembles <i>T. sagittifera</i> <b>n. comb.</b> but can be differentiated from the latter species on the basis of several morphological characteristics that are discussed above in the remarks section of the latter species.</p>Published as part of <i>Patel, Krupal, Naderloo, Reza, Trivedi, Jigneshkumar & Mitra, Santanu, 2023, On the taxonomy of Philyra sagittifera (Alcock, 1896) and P. concinnus Ghani & Tirmizi, 1995 (Decapoda, Brachyura, Leucosiidae), with description of a new genus from the Indian Ocean, pp. 430-440 in Zootaxa 5330 (3)</i> on pages 436-439, DOI: 10.11646/zootaxa.5330.3.6, <a href="http://zenodo.org/record/8254906">http://zenodo.org/record/8254906</a>
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