103 research outputs found
Enhanced Oil Recovery using Carbonated Polymeric Nanofluids : A New Age Approach to CO2 Utilization and Corrosion Mitigation
No full textThe author would like to thank the CIF facility and its staff, including Mr. Anuj Prajapati, Mr. Zahoor Alam, and Mr. Brijesh. Thanks are also extended to all the members associated with the work.Peer reviewe
Semantic network based knowledge representation for cognitive decision making in teaching electrical motor concepts
No full textDeveloping E. coli-E. coli co-cultures to overcome barriers of heterologous tryptamine biosynthesis
No full textTryptamine is an alkaloid compound with demonstrated bioactivities and is also a precursor molecule to many important hormones and neurotransmitters. The high efficiency biosynthesis of tryptamine from inexpensive and renewable carbon substrates is of great research and application significance. In the present study, a tryptamine biosynthesis pathway was established in a metabolically engineered E. coli-E. coli co-culture. The upstream and downstream strains of the co-culture were dedicated to tryptophan provision and conversion totryptamine, respectively. The constructed co-culture was cultivated using either glucose or glycerol as carbon source for de novo production of tryptamine.The manipulation of the co-culture strains’ inoculation ratio was adapted to balance the biosynthetic strengths of the pathway modules for bioproduction optimization. Moreover, a biosensor-assisted cell selection strategy was adapted to improve the pathway intermediate tryptophan provision by the upstream strain, which further enhanced the tryptamine biosynthesis. The resulting biosensor-assisted modular co-culture produced 194mg/L tryptamine with a yield of 0.02 g/g glucose using shake flask cultivation.The findings of this work demonstrate that the biosensor-assisted modular co-culture engineering offers a new perspective for conducting microbial biosynthesis.Peer reviewe
Design and Governance of Platforms to leverage an ecosystem of complementors
No full textThis paper analyzes how specific aspects of design and governance of platform explain complementors participation in the video game console industry. This study introduces a new dimension to measure platform openness and suggest that by opening the platform vertically to different markets, platform firms can smoothen complementors competition and incentivize them to participate in their network. This study makes a first attempt to measure platform boundary resources and highlight its positive influence on complementors participation. Finally, the analysis also explores conditions under which signing an exclusive contract is beneficial for complementors. Complementors tend to participate in exclusive contracts during the early stages of platform rather than mature stages due to intense competition among complementors in the latter stage. The results suggest that by following the correct design and governance strategies, platform firms can orchestrate a large network of complementors and proliferate a variety of complementary product offerings
Stability-indicating reversed-phase high-performance thin-layer chromatography/densitometry estimation of lafutidine in bulk and tablets
No full textLafutidine (LTD) is a histamine H2 receptor antagonist, with anti-ulcer and mucosal protective activity. A sensitive and selective stability-indicating reversed- phase high -performance thin-layer chromatography (RP-HPTLC)/Densitometry method is suggested for the determination of LTD in the presence of its acid, alkaline, oxidative and dry heat degradation products. Stress testing of LTD was done according to the International Conference on Harmonization (ICH) guidelines in order to validate the stability-indicating power of the analytical procedures. Stress testing demonstrated that LTD underwent acid, alkaline, oxidative and dry heat degradation; on the other hand, it showed stability towards neutral and photo degradation. Chromatography was performed on aluminum-backed silica gel 60 RP-18 F254 S plates with Methanol: Water: Triethylamine 8: 2: 0.5 (v/v) as mobile phase. Densitometric scanning was performed at 276 nm. The system gave compact bands for LTD (Rf 0.67 ± 0.02). Linear regression analysis data for the calibration plots discovered a good linear relationship with r2 > 0.99 in the working concentration range 500 to 3000 ng per band. The limits of detection and quantification were 23.51 ng and 71.26 ng, respectively. The developed method can routinely be used for analysis of LTD in bulk and pharmaceutical formulations
Ontology Based Knowledge Representation for Cognitive Decision Making in Teaching Electrical Motor Concepts
No full texthe Creators A Look at the Changing Work of Potters and the Future of Their Craft in Thimi, Nepal
No full textThe Newari sur-name Prajapati has been associated with those who are of the potter caste in the Kathmandu valley. In the past 30 years ceramics in the historic pottery town of Thimi has changed drastically from being an essential and necessary craft and the only occupation for Prajapatis, to a struggling population of visually aging potters. This paper examines the workshop Everest Pottery in Thimi nepal as a case study for the state of ceramics in Thimi today. The author traces the origins of the workshop\u27s founder Shiva Prajapati and examines the shift that Shiva made from traditional Newari pottery practices to modern techniques. The author discusses the success of Everest Pottery and the shift of their market from local to global. The author concludes that with the rise of aluminum, plastic and the fact that Nepali\u27s are no longer looking to buy terracotta pots, the traditional forms that have inhabited the houses of the Kathmandu Valley for generations may be all but gone in the next few years. As a secondary focus the author discovered that while Everest Pottery has left behind most traditional Newari pottery practices, Newari traditional values and customs remain present in the workplace and can be witnessed through the stark division of labor by gender. The women of Everest Pottery all have different stories of how they came to work in ceramics. The author discusses the stories of the women of Everest Pottery, their perceptions of their own roles in ceramics and the perceptions of the female role from the point of view of the men of Everest Pottery. The author found that while there is no spoken rule against women throwing on the wheel, even in a modern ceramic workshop, this tradition remains. Most women who learned to throw in their youth stop throwing when they get married, and additionally do not want to try again for fear that it is too difficult. Similarly, the men of Everest Pottery express doubts about women\u27s ability to throw due to their lack of strength. While the women of Everest Pottery seem to be happy with the status quo, the author offers a counter observation that while some women aren\u27t interested in the wheel, the ones that are given no space to learn. Additionally the author offers potential futures for ceramics if women are eventually included in this integral part of the process
Climate And Environmental Changes: Restrospect And Prospect
No full textDiscussion on problems of climate and environmental changes i.e. global warming has inundated in recent years. Every major newspaper publishes multiple editorials or op- ed pieces on the topic, the broadcast media and researchers regularly discuss the issue, and thousands of Web pages and blogs provide definitions and information and suggest causes and consequences of action and inaction. Under the situation why are we adding to congestion on the subject? Because the analysis of past has much to contribute, especially in understanding prospects of adapting to climate change, which has received relatively little study and comment relative to the standing it should take in the debate [Gary D. Libecap and Rechard H. Steckel, 2011]. At the outset, it is to be noted that in the present paper science of climate change is not evaluated as author is not the expertise to contribute on this matter but the paper respond to economic effects of the climate and environmental changes
Rutin Potentially Binds the Gamma Secretase Catalytic Site, Down Regulates the Notch Signaling Pathway and Reduces Sphere Formation in Colonospheres
No full textRutin, a natural flavonol, can modulate molecular signaling pathways and has considerable potential in cancer treatment. However, little is known about the effect of rutin on the notch signaling pathway (NSP) in cancer and cancer stem-like cells. In this study, we explored the effect of rutin on gamma secretase (GS, a putative notch signaling target) inhibition mediated NICD (Notch Intracellular Domain) production in colon cancer cells. Molecular docking, MM-GBSA, and Molecular dynamics (MD) simulation experiments were performed to check rutin’s GS catalytic site binding potential. The HCT-116 colon cancer and cancer stem-like cells (colonospheres) were utilized to validate the in silico findings. The NICD production, notch promoter assay, expression of notch target genes, and cancer stemness/self-renewal markers were studied at molecular levels. The results were compared with the Notch-1 siRNA transfected test cells. The in silico study revealed GS catalytic site binding potential in rutin. The in vitro results showed a decreased NICD formation, an altered notch target gene (E-cad, Hes-1, and Hey-1) expression, and a reduction in stemness/self-renewal markers (CD44, c-Myc, Nanog, and Sox2) in test cells in a time and dose-dependent manner. In conclusion, rutin inhibits the notch signaling pathway and reduces the stemness/self-renewal property in colon cancer cells and the colonospheres by targeting gamma secretase. The clinical efficacy of rutin in combination therapy in colon cancer may be studied in the future
Minimized Group Delay FIR Low Pass Filter Design Using Modified Differential Search Algorithm
No full textDesigning a finite impulse response (FIR) filter with minimal group delay has proven to be a difficult task. Many research studies have focused on reducing pass band and stop band ripples in FIR filter design, often overlooking the optimization of group delay. While some works have considered group delay reduction, their approaches were not optimal. Consequently, the achievement of an optimal design for a filter with a low group delay value still remains a challenge. In this work, a modified differential search optimization algorithm has been used for the purpose of designing a minimal group delay FIR filter. The results obtained have been compared with the classical techniques and they turned out to be promising
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