1,721,041 research outputs found

    I Controlli Sanitari nella Filiera Avicola: il punto di vista dell'Università

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    Art. 10. del D.M. 13-11-2013 Registrazione (data, motivo e, in caso di non conformità, l’esito) dei controlli eseguiti dai servizi veterinari: verifiche periodiche di frequenza e numerosità in base alla valutazione del rischio attribuito all’azienda in relazione ai parametri epidemiologici, la capacità strutturale e le altre condizioni sanitarie rilevanti. Il riconoscimento sanitario degli stabilimenti di pollame ad effettuare scambi è regolamentato da: - D. Lgs. 3 dicembre 2014, n. 199 fissa le norme di polizia sanitariaI Sistemi informativi permettono di aumentare la qualità, la precisione, la disponibilità e la tempestività dei dati relativi agli animali vivi, agli alimenti di origine animale e ai mangimi, nonché di effettuare le necessarie valutazioni epidemiologiche per una razionale e funzionale gestione della prevenzione e del controllo della malattie animali. L'art. 4 D. Lgs. n. 9 del 25 gennaio 2010 rende obbligatoria la registrazione degli allevamenti avicoli nella Banca Dati Nazionale (BDN), mentre il D.M. del 13-11-2013 detta le modalità operative di funzionamento dell’anagrafe informatizzata delle aziende avicole. La registrazione del riconoscimento ad effettuare scambi comunitari e dello stato sanitario dell’allevamento viene regolamentato dalle seguenti norme: Art. 4.2.1. del D.M. 13-11-2013 ... omissis ... Il servizio veterinario competente registra lo stato sanitario per Salmonellosi con data dell’attribuzione/aggiornamento dell’accreditamento per allevamenti di Gallus gallus e Meleagris gallopavo sottoposti ai piani di controllo delle Salmonellosi; Art. 7. Registrazione delle movimentazioni in B.D.N. ... omissis ... La validazione in B.D.N. della “richiesta di movimentazione in uscita” da parte del Servizio veterinario A.S.L., nei casi previsti, equivale all’“attestazione sanitaria” della documentazione di accompagnamento degli avicoli; per gli scambi intracomunitari e le importazioni in provenienza dai Paesi terzi di pollame e uova da cova.Le Regioni e le Province Autonome, ai sensi dell’art. 3.1 del D.lg. 199/2014, rilasciano il riconoscimento e registrano tale informazione in BDN; Art. 5 - Requisiti generali per uova da cova, pulcini di un giorno e pollame riproduttore e da reddito 1. ...omissis... sono oggetto di scambi intracomunitari esclusivamente le uova da cova, i pulcini di un giorno, il pollame riproduttore e da reddito appartenenti ad un branco che, al momento della spedizione, non presenta alcun sintomo clinico o sospetto di malattia contagiosa per il pollame e proviene da stabilimenti che soddisfano i seguenti requisiti ...omissis... &nbsp

    Amphixenosic aspects of Staphylococcus aureus infections in Man and Animals: veterinary and public health perspectives

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    From a veterinary and public health perspective, Staphylococcus aureus (SA) is a major concern. As opportunistic pathogen responsible for severe nosocomial and community-associated infections of humans, it is one of the most important pathogenic Staphylococcus species in veterinary medicine, especially where animal-derived food products intersect humans. According to the mode of transmission, SA infection between hosts is classified as “direct zoonoses”; actually, the correct definition of SA infections between humans and animals is “amphixenoses” or infections maintained in both man and lower vertebrate animals and transmitted in either direction. SA presents a high genetic plasticity. In the last years, in addition to the genetic lineages associated to the hospital or community in humans, other lineages have emerged in the animal–human interface. Moreover, SA can harbor a wide variety of resistance genes, at least 60 different resistance genes that confer resistance to virtually all classes of antimicrobial agents approved for use in humans and animals. Multidrug-resistant strains, in particular MRSA, have become established as a veterinary pathogens in pets and horses; in livestock, MRSA presents a concern for public health as a reservoir that can infect humans and as a source of transferrable resistance genes. In addition to, SA has been also recognized as intracellular pathogen and biofilm producer. This property potentially contributes to bacterial persistence, protection from antibiotics, and evasion of immune defenses. Based on this evidence, animal-adapted SA lineages exhibiting resistance to antibiotics must be considered a major threat to public health

    Autologous platelet-rich plasma for topical application as regenerative therapy in dogs

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    This protocol describes a step-by-step procedure for the production of autologous platelet-rich plasma for topical application in dogs

    Performance Evaluation of an Indirect milk-ELISA for the Diagnosis of Mycobacterium Avium Subsp. Paratuberculosis in Individual Samples of Dairy Sheep

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    The aim of this study was to evaluate the performance and the most appropriate cut-off value in dairy sheep samples of an indirect ELISA (ID Screen® Paratuberculosis - ID.vet, France). For the purpose, an intra-flock epidemiological study was carried out in Italy on 200 paired blood and milk sheep sera and 200 faecal samples collected both in October 2019 (T0: low milk production) and in April 2020 (T6: high milk production) in two flocks ..

    Spreading of Macrorhabdus Ornithogaster in Ornamental Bird Aviaries in Piedmont

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    Avian gastric yeast (AGY) Macrorhabdus ornithogaster, has a worldwide distribution and currently colonize the proventriculus and ventriculus of a wide of bird species (PHALEN, 2014), representing a potential threat to bird breeders. The aim of this study was to assess the presence of AGY in breeding ornamental birds, rearing in Piedmont. The study was conducted in 15 breeding centers of ornamental birds and on birds from 3 private owners. Pool of fecal samples were examined by microscopic test for the presence of yeast. Level of shedding of AGY was evaluated using a semi-quantitative scale (LANZAROT et al., 2013). On some dead animals was performed histological examination. The presence of AGY was observed in 12 of the 18 farms (67%): 61% in commercial farms and 50% in private ones. The prevalence was 61%, in farms with 1 species, while in multispecies farms the prevalence was 44%. A high number of fecal parasitic and fungal co-infections (62%) was observed. From dead animals, the histological exam confirmed AGY infection. The study confirms that M. ornithogaster is widespread: 2/3 of farms and more than half of the sampled cages harbored AGY. Moreover, the parasitic and fungal co-infections do not seem to influence the risk of infection for AGY (P=0.759)

    Longitudinal Study on Mycobacterium Avium Subsp. Paratuberculosis Antibody Kinetics and Excretion in Dairy Sheep Using Blood, Milk and Faeces Throughout the Lactation Period

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    The dynamics of immune responses and excretion of Mycobacterium avium subsp. paratuberculosis (MAP) during the different stages of natural infection were evaluated in an intra-flock longitudinal study among asymptomatic Italian dairy sheep. In October 2019 (T0), April 2020 (T6), and June 2021 (T20), a cross-sectional and prospective incidence studies were performed on 150 ewes. ..

    Evaluation of the association between the lactation stage and serum and milk ELISA results in the diagnosis of ovine Paratuberculosis

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    In order to determine the association between the lactation stage with serum and milk ELISA results, 697 ewes reared in 17 paratuberculosis infected dairy farms, were sampled: 10 flocks at early/late and 7 flocks at peak lactation period. Prevalences of 7.75% (n=697) and 5.31% (n=697) were observed analyzing serum and milk samples, respectively, and the difference was not significant (P=0.065). A higher number of Mycobacterium avium subsp. paratuberculosis (MAP) seropositive sheep were found in the larger flocks than in the smaller ones (P=0.04), when sampled in early/late lactation. Moreover, the milk ELISA revealed that 7.8% and 3.7% of the ewes were positive to MAP antibodies at early/late and peak of lactation, respectively, and the difference was highly significant (P=0.021). Considering the importance of early diagnosis, milk ELISA could be a valid alternative tool in early/late lactation stage. Further studies, investigating the effect of MAP positivity on production parameters of ovine dairy breeds, are needed

    Platelet-rich Plasma and Other Hemocomponents in Veterinary Regenerative Medicine

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    Platelet-rich plasma, platelet-rich fibrin, fibrin glue, and platelet lysate are the most widely used nontransfusional hemocomponents that, as biological and therapeutic aids, enhance the physiological reactions after an injury to facilitate the repair and regeneration processes. Recently, this type of therapy also has significantly expanded in veterinary medicine. Due to many similarities, the animal patient could be a good reference as a study model for humans, especially for chronic and difficult-to-heal injuries. This review discusses the current state of hemocomponent use for topical application in veterinary medicine, with a comparison with human medicine

    Health assessment of wild speckled dwarf tortoises, Chersobius signatus.

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    BACKGROUND In free-ranging reptile populations, bacterial, fungal, viral and parasitic pathogens may affect hosts through impairment in movements, thermoregulation, reproduction, survival, and population dynamics. The speckled dwarf tortoise (Chersobius [Homopus] signatus) is a threatened species that is mostly restricted to the Succulent Karoo biome in South Africa, and little information on pathogens of this species is available yet. We derived baseline parameters for five males and five females that were captured to genetically enhance a conservation breeding program in Europe. Upon collection of the tortoises, ticks were removed and identified. Immediately upon arrival in Europe, ocular, nasal, oral and cloacal swabs were taken for viral, bacteriological and mycological examinations. Fecal samples were collected before and 1 month after fenbendazole treatment, and analyzed for parasites. A panel of PCR, aiming to detect herpesviruses, adenoviruses and iridoviruses, was carried out. RESULTS Samples were negative for viruses, while bacteriological examination yielded detectable growth in 82.5% of the swabs with a mean load of 16 × 107 ± 61 × 108 colony forming units (CFU) per swab, representing 34 bacterial species. Cloacal and oral swabs yielded higher detectable growth loads than nasal and ocular swabs, but no differences between sexes were observed. Fungi and yeasts (mean load 5 × 103 ± 13 × 103 CFU/swab) were detected in 25% of the swabs. All pre-treatment fecal samples were positive for oxyurid eggs, ranging from 200 to 2400 eggs per gram of feces, whereas after the treatment a significantly reduced egg count (90-100% reduction) was found in seven out of 10 individuals. One remaining individual showed 29% reduction, and two others had increased egg counts. In five tortoises, Nycthocterus spp. and coccidian oocysts were also identified. Soft ticks were identified as Ornithodoros savignyi. CONCLUSIONS Our baseline data from clinically healthy individuals will help future studies to interpret prevalences of microorganisms in speckled dwarf tortoise populations. The study population did not appear immediately threatened by current parasite presence
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