523 research outputs found
Elevated expression of serine protease HtrA1 in preeclampsia and its role in trophoblast cell migration and invasion
No full textObjective: Aberrant expression of developmentally regulated genes during placental development could affect fetal growth and contribute to preeclampsia. Expression of serine protease HtrA1 is developmentally regulated with the highest expression in decidua capsularis, compared with ectoplacental cone, and with the highest expression during later stages of pregnancy, compared with early stages. This study was designed to determine the expression of HtrA1 in placental tissues from control and preeclamptic pregnancies and to determine the effect of HtrA1 expression in trophoblast cell migration and invasion. Study Design: HtrA1 expression was assessed by immunohistochemistry in placentas from gestational age-matched preeclamptic and control pregnancies. HtrA1 expression in extravillous trophoblast cells, HTR-8/SVneo, was assessed by immunoblotting and immunofluorescence microscopy. Finally, the effect of ectopic expression of HtrA1 on cell migration and invasion was determined in HTR-8/SVneo cells. Results: Higher expression of HtrA1 was detected in placental tissues collected from patients with early-onset preeclampsia, compared with those from gestational age-matched control samples. Moreover, ectopic expression of HtrA1 significantly attenuates HTR-8/SVneo cell migration and invasion. Conclusion: Higher expression of HtrA1 is associated with early-onset preeclampsia and may affect trophoblast cell migration and invasion. © 2008 Mosby, Inc. All rights reserved
Direct numerical simulation of turbulent Couette-Poiseuille flow with zero skin friction
No full textThe near-wall scaling of mean velocity U(y) is addressed for the case of zero skin friction on one wall of a fully turbulent channel flow. The present DNS results can be added to the evidence in support of the conjecture that U is proportional to √yw in the region just above the wall at which the mean shear dU/dy = 0
Combination Of Cfd And Csd Packages For Fluid-Structure Interaction
No full textIn this article the UDF script file in the Fluent software was rewritten as the "connecting file" for the Fluent and the ANSYS/ABAQUS in order that the joined file can be used to do aero-elastic computations. In this way the fluid field is computed by solving the Navier-Stokes equations and the structure movement is integrated by the dynamics directly. An analysis of the computed results shows that this coupled method designed for simulating aero-elastic systems is workable and can be used for the other fluid-structure interaction problems
An integrated model of the transcriptome of HER2-positive breast cancer
No full textOur goal in these analyses was to use genomic features from a test set of primary breast tumors to build an integrated transcriptome landscape model that makes relevant hypothetical predictions about the biological and/or clinical behavior of HER2-positive breast cancer. We interrogated RNA-Seq data from benign breast lesions, ER+, triple negative, and HER2-positive tumors to identify 685 differentially expressed genes, 102 alternatively spliced genes, and 303 genes that expressed single nucleotide sequence variants (eSNVs) that were associated with the HER2-positive tumors in our survey panel. These features were integrated into a transcriptome landscape model that identified 12 highly interconnected genomic modules, each of which represents a cellular processes pathway that appears to define the genomic architecture of the HER2-positive tumors in our test set. The generality of the model was confirmed by the observation that several key pathways were enriched in HER2-positive TCGA breast tumors. The ability of this model to make relevant predictions about the biology of breast cancer cells was established by the observation that integrin signaling was linked to lapatinib sensitivity in vitro and strongly associated with risk of relapse in the NCCTG N9831 adjuvant trastuzumab clinical trial dataset. Additional modules from the HER2 transcriptome model, including ubiquitin-mediated proteolysis, TGF-beta signaling, RHO-family GTPase signaling, and M-phase progression, were linked to response to lapatinib and paclitaxel in vitro and/or risk of relapse in the N9831 dataset. These data indicate that an integrated transcriptome landscape model derived from a test set of HER2-positive breast tumors has potential for predicting outcome and for identifying novel potential therapeutic strategies for this breast cancer subtype
Finite-Sample Bias Propagation in Autoregressive Estimation With the Yule–Walker Method
No full textThe Yule-Walker (YW) method for autoregressive (AR) estimation uses lagged-product (LP) autocorrelation estimates to compute an AR parametric spectral model. The LP estimates only have a small triangular bias in the estimated autocorrelation function and are asymptotically unbiased. However, using them in finite samples with the YW method for AR estimation can give a strong distortion in the weak parts of the power spectral density. The distortion is shown to be influential in an example without strong spectral peaks. The true biased AR model, which is computed by applying the triangular bias to the true autocorrelation function, has an infinite order. A new objective measure is introduced to determine the smallest sample size for which the unbiased asymptotic theory can be considered as a fair approximation.Multi-Scale PhysicsApplied Science
XML approach to communication design of WebGIS
No full textXML can describe the concept model of inclusion relationship conveniently. Also, it can directly express the concept model in an understandable way, and the expression format is so flexible that no useless element will be included there. While describing communication protocols by XML, we can not only give a common format for data and control commands, but also reuse the existing XML-parsers, so as to facilitate the expansibility and integration of protocols in a system, W3C had proposed Simple Object Access Protocol (SOAP) [1], which is a light weight protocol based on XML used to build information exchange framework under distributed environments. ArcInfo's ArcIMS also used ArcXML as the fundamental command and data transmission protocols to communicate between users' web pages and backend spatial data servers [2]. Our idea of XML based communication protocols for WebGIS benefits from the SOAP model, but we basically focus on the application in WebGIS. With the aid of UML, the typical requiring and responding protocols of WebGIS are analyzed firstly. Then the mechanism of designing communication protocols following W3C's XML Schema specification is illustrated.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000231171300077&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Computer Science, Information SystemsComputer Science, Theory & MethodsSCI(E)CPCI-S(ISTP)
State-Space Inference and Learning with Gaussian Processes
No full text18.10.13 KB. Ok to add author version to spiral, authors hold copyright.State-space inference and learning with Gaussian processes (GPs) is an unsolved problem. We propose a new, general methodology for inference and learning in nonlinear state-space models that are described probabilistically by non-parametric GP models. We apply the expectation maximization algorithm to iterate between inference in the latent state-space and learning the parameters of the underlying GP dynamics model. Copyright 2010 by the authors
Spatial information grid - An agent framework
No full textSpatial information grid (SIG) is a spatial information infrastructure that has the capability of providing services on-demand. In this paper, agent technology is adopted to construct a SIG framework, which contains three layers: users/applications layer, agent services layer and information layer. Different applications can get their spatial information via agent services, and agent services make the procedure of navigating and accessing spatial information transparent to users. Also, the implementation issues of the framework are discussed.Computer Science, Artificial IntelligenceComputer Science, Information SystemsComputer Science, Theory & MethodsSCI(E)CPCI-S(ISTP)
A component-based WebGIS Geo-Union
No full textA component-based WebGIS system Geo-Union is introduced, including its architecture, functional partition and web application mode.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000223024600075&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Computer Science, Information SystemsComputer Science, Software EngineeringComputer Science, Theory & MethodsSCI(E)CPCI-S(ISTP)
A cache mechanism for component-based WebGIS
No full textFirst, a component-based WebGIS system Geo-Union is introduced, then the architecture and the web application mode of Geo-Union are discussed. After that, a spatial cache framework is designed and adopted in Geo-Union to improve its performance in network environment. There are three levels of spatial cache: spatial database cache, network spatial cache and spatial data proxy server, and they play different roles in different situations to improve spatial data access performance of Geo-Union.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000223079700121&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Computer Science, Interdisciplinary ApplicationsComputer Science, Theory & MethodsSCI(E)CPCI-S(ISTP)
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