593 research outputs found
Generation and analysis of transgenetic mice expressing catalytically inactive recombination activating gene-1
No full textThe proteins encoded by the recombination activating genes (RAG1 and RAG2) are lymphoid cell-specific endonucleases that initiate V(D)J recombination; the mechanism by which the exons that encode the variable portion of both B and T cell receptors are assembled somatically from several variable (V), joining (J) and sometimes diversity (D) germline gene segments. The enzymatic activity of the RAG proteins has been attributed to three acidic residues (Asp600, Asp708, and Glu962) within RAG1. These residues constitute the DDE motif which functions as the active site for the RAG proteins by coordinating metal ions required for catalysis of DNA cleavage. Mutation of any of these residues abrogates V(D)J recombination without affecting the DNA binding activity of the RAG protein complex. Accordingly, we have speculated that ectopic expression of a catalytically inactive form of RAG1 in vivo may suppress the activity of endogenous RAG1 and V(D)J recombination in a dominant negative pattern.
To test this hypothesis we have generated transgenic mice overexpressing a catalytically deficient form of RAG1 in which the three residues comprising the DDE motif have been mutated to alanine. We show here that transgenic mice, but not non- transgenic littermates, accumulate an unusual population of B cells that are characterized by low expression of the pan B cell marker, CD45R (B220), referring to them as B220k> B cells. Extensive phenotypic characterization using How cytometery shows that the B22010 B cells are phenotypically resemble the immature transitional- and Bl- B cell. Total splenocytes and sorted splenic B22010 B cells of transgenic mice showed significant reduction in their proliferative capacity in response to B cell receptor cross-linking. Moreover, serum levels of immunoglobulins (IgM and IgG) are significantly reduced in transgenic mice when compared to non-transgenic littermates.
Taken together, these findings are consistent with significant impairment in B cell maturation due to suppression of secondary rearrangement mediated by the RAG proteins to edit receptor specificity in self-reactive B cells. This model may provide an opportunity to dissect the mechanisms of tolerance and autoimmunity in peripheral B cellsProQuest Traditional Publishing Optionxvii, 158 page
sj-docx-1-jic-10.1177_08850666221109779 - Supplemental material for Airway Pressure Release Ventilation for Acute Respiratory Failure Due to Coronavirus Disease 2019: A Systematic Review and Meta-Analysis
No full textSupplemental material, sj-docx-1-jic-10.1177_08850666221109779 for Airway Pressure Release Ventilation for Acute Respiratory Failure Due to Coronavirus Disease 2019: A Systematic Review and Meta-Analysis by Ashraf Roshdy, Ahmad Samy Elsayed and Ahmad Sabry Saleh in Journal of Intensive Care Medicine</p
VisionArtech - 5th Honorary Mention
No full textA new building skin has been proposed that addresses the challenge of reducing cooling load and daylight needs of buildings in hot climatic areas. It represents a multi-disciplinary research-based design that links between architecture, biomimicry, and computational design.
Ideas from nature serve as inspiration in designing a porous, folded double façade for an existing building in Cairo, aiming at increasing heat lost by convection in the cavity as well as reducing heat gained by radiation. The folded surface provides self-shading while a network of small perforations provides airflow, allowing the cavity to breathe and remove heated air.
The cooling load and daylight performances of an existing room with a single façade were compared to a flat double façade and to our proposal.
Results show a strong reduction of cooling loads by up to 12%, an increase in daylighting by 24% and a reduction of over-lit space by 53%.
Our proposal improves wellbeing of building occupants, which increases productivity, introduces more advanced construction technologies into the building sector, establishes a sense of belonging to a global community that cares for the Environment and enhances local aesthetic urban quality
Penetration vs. Shear wave velocity for evaluation of liquefaction potential in Northeast Arkansas
No full textShear wave velocity profiles at 16 bridge sites in Northeast Arkansas (NEA) were determined using a hybrid, non-invasive technique. These profiles were used to evaluate the liquefaction resistance at the selected sites using the simplified procedure by Seed and Idriss (Vs approach). The liquefaction resistance was also evaluated using the Standard Penetration Test (SPT approach) results from the geotechnical investigations at these sites that were conducted by the Arkansas State Highway and Transportation Department (AHTD). The Liquefaction Potential Index (LPI), as introduced by Iwasaki, was used to evaluate the severity of liquefaction. The results of both approaches were then compared. Recommendations were made to AHTD personnel for liquefaction evaluation of future bridge projects based on the results of this research. © Elsayed and Pezeshk; Licensee Bentham Open
Effect of Psychological First Aid Intervention Program on Post-traumatic Growth¬ among Recently Diagnosed Cancer Patients
No full textAbstract: Cancer diagnosis and related treatment represent one of the most painful and distressing events that human beings can meet in their life. This causes a serious crisis in the lives of patients, their families, and those around them. This study aims to evaluate the effect of psychological first aid intervention program on post-traumatic growth among recently diagnosed cancer patients. Study design: A quasi-experimental design was utilized. Setting: The study conducted in the following hospitals at Ismailia city: Suez Canal University Hospitals and Ismailia Oncology Hospital. Subject: A sample of 52 recently diagnosed cancer patients. Tools of data collection: Two tools were used: Tool (I) demographic characteristics and medical background. Tool (II) Post-traumatic Growth Inventory (PTGI). Results: Findings revealed that, recently diagnosed cancer patients had low level of post-traumatic growth in pre-program phase while, post and follow up phases, the results revealed that patients had high level of post-traumatic growth. Conclusion: Psychological first aid intervention program had significant effect on improvement of post-traumatic growth of recently diagnosed cancer patients in post, and follow up study phases compared with pre-program phase. Recommendation: Psychological first aid based interventions can be combined with care programs of hospitals and daily clinical works as a complementary intervention.
Keywords: Cancer, Post-traumatic growth, Psychological first aid, Recently diagnosed.
Title: Effect of Psychological First Aid Intervention Program on Post-traumatic Growth among Recently Diagnosed Cancer Patients
Author: Mahmoud Abd Elmagied Ahmed, Ola Ali Abd El-Fattah, Ashraf Mohamed Mohamed Ali El-tantawy, Somaya Elsayed Abou-Abdou
International Journal of Novel Research in Healthcare and Nursing
ISSN 2394-7330
Vol. 9, Issue 3, September 2022 - December 2022
Page No: 41-54
Novelty Journals
Website: www.noveltyjournals.com
Published Date: 26-September-2022
DOI: https://doi.org/10.5281/zenodo.7112904
Paper Download Link (Source)
https://www.noveltyjournals.com/upload/paper/Effect%20of%20Psychological%20First%20Aid-26092022-1.pdfInternational Journal of Novel Research in Healthcare and Nursing, ISSN 2394-7330, Novelty Journals, Website: www.noveltyjournals.co
XRWALC 12 - KIDSTAR: Augmented Reality Reading Tool To Measure Children's Behavior
No full textABSTRACT
For decades, reading retained to be the best tool to enhance children’s imagination. Our presented solution—KIDSTAR uses augmented reality technology to enhance children’s reading and measure children’s interaction behaviour during the reading experience. KIDSTAR accommodates children with augmented reality interactive tools to illustrate and interact with the augmented scenes. These tools enable users to alter virtual objects based on their preferences while tracking their decisions and choices. The innovative contribution of KIDSTAR is the merging of augmented reality reading experience with analytical feedback. This merge converts the reading experience into a two-way creative channel and a unique platform for unleashing children’s behaviour.
HOW TO CITE (APA)
Elsayed, N., Elkady, M. & Shewail, A.S. (2022). KIDSTAR: Augmented Reality Reading Tool To Measure Children's Behavior. In 1st Int. Workshop on Analytics, Learning & Collaboration in eXtended Reality - ACM International Conference on Interactive Media Experiences: IMX 2022 (pp. 257-262). Aveiro, Portugal. </p
Once upon Palestine - Audio documentary
No full textOnce Upon Palestine is an audio documentary that sheds light on a very personal aspect in my life. I personally draw back to the history of the Palestinian Nakba (catastrophe) with the assistance of Professor Ashraf ElSherif from the Political Science Department at the American University in Cairo. This documentary provides an insight to the Egyptian perspective on the Palestinian cause
Lane Line Detection and Object Scene Segmentation Using Otsu Thresholding and the Fast Hough Transform for Intelligent Vehicles in Complex Road Conditions
Get PDFAn Otsu-threshold- and Canny-edge-detection-based fast Hough transform (FHT) approach to lane detection was proposed to improve the accuracy of lane detection for autonomous vehicle driving. During the last two decades, autonomous vehicles have become very popular, and it is constructive to avoid traffic accidents due to human mistakes. The new generation needs automatic vehicle intelligence. One of the essential functions of a cutting-edge automobile system is lane detection. This study recommended the idea of lane detection through improved (extended) Canny edge detection using a fast Hough transform. The Gaussian blur filter was used to smooth out the image and reduce noise, which could help to improve the edge detection accuracy. An edge detection operator known as the Sobel operator calculated the gradient of the image intensity to identify edges in an image using a convolutional kernel. These techniques were applied in the initial lane detection module to enhance the characteristics of the road lanes, making it easier to detect them in the image. The Hough transform was then used to identify the routes based on the mathematical relationship between the lanes and the vehicle. It did this by converting the image into a polar coordinate system and looking for lines within a specific range of contrasting points. This allowed the algorithm to distinguish between the lanes and other features in the image. After this, the Hough transform was used for lane detection, making it possible to distinguish between left and right lane marking detection extraction; the region of interest (ROI) must be extracted for traditional approaches to work effectively and easily. The proposed methodology was tested on several image sequences. The least-squares fitting in this region was then used to track the lane. The proposed system demonstrated high lane detection in experiments, demonstrating that the identification method performed well regarding reasoning speed and identification accuracy, which considered both accuracy and real-time processing and could satisfy the requirements of lane recognition for lightweight automatic driving systems
Correction: Guidelines for the prevention, detection and management of the renal complications of COVID-19 in Africa
Get PDFThe authors of the article ‘Guidelines for the prevention, detection and management of the renal complications of COVID-19 in Africa’ [1] wish to acknowledge the contribution of Professor Hussein El Fishawy. Our guidelines drew on various sources, including the Egyptian Ministry of Health guidelines, portions of which were adapted and reproduced with permission from the Egyptian Ministry of Health. Two of the authors of those guidelines, Professors Elsayed and Zaki, are also coauthors of our paper. Professor El Fishawy was the third author of the Egyptian guidelines and we would like to acknowledge his contribution to our review through this source, especially with respect to the treatment algorithms for patients with kidney transplants and those with acute kidney injury. Reference1. Elsayed HM, Wadee S, Zaki MS, Were AJO, Ashuntantang GE, Bamgboye EL, et al. Guidelines for the prevention, detection and management of the renal complications of COVID-19 in Africa. Afr J Nephrol. 2020; 23(1):109-126
Représentations littéraires du sacré dans le roman maghrébin de langue française
No full textThis interdisciplinary study explores how Driss Chraïbi’s L’Homme du Livre (1995), Assia Djebar’s Loin de Médine (1991), and Anissa Boumediène’s La fin d’un monde (1991) present accounts of particular historical moments in early Islam. This study explores the role of the imagination as well as freedom of invention when reconstructing historical events. It engages the novels through a study of the interplay between the literary text and the sources and traditions that impact and shape the text narrative. Gaining direct access to the original sources in Arabic serves to analyze how religious and early historical materials are considered in and reflected by the fictional texts. Because the sources tend to differ in both content and approach, this study examines their preoccupations in order to determine the criteria of selection applied by each novelist.Ph.D.Includes bibliographical referencesIncludes vitaby Hanan Elsaye
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