122 research outputs found
Prediction of potential barcoding sites on ITS1 by wavelet transform
No full text<p>For sustainable development, biodiversity conservation and life-quality improvement must be simultaneously considered. Molecular techniques have greatly impacted biotechnology. These methods have, in particular, improved the capability to investigate the fine differences among organisms and, as a consequence, to better investigate the effects on environmental factors on them. We propose an approach to support the optimal selection of molecular probes for barcoding application in many biotechnological fields. The aim of our work is specificity maximization. To this purpose, we have integrated a filter system based on wavelet transforms with biological knowledge about the sequence proneness to mutation and post-translational modification. Specifically, we have tested the proposed method on ITS1 sequences that are a region of the rRNA locus. Our analysis has shown the presence of other local relative stable conformations in addition to known cleavage site. Their characteristics differ within the group of mammals selected for our analysis. These variations could be used to design new species-specific barcoding probes or other quick molecular screening tools.</p
SNP analysis of Rac1 For personalized ligand interaction
No full textThis paper addresses mutational events that give rise to differing response to drugs focusing on Rac1, a protein that has been recognized as a target for drug design for cardiovascular disease due its regulatory role of angiogenesis. Rac1 has been considered with reference to Single Nucleotide Polymorphism (SNP), which has become of great value for personalized medicine. We have considered four variation of Rac1 registered in UNIPROTKB. Two of these variations are due to the environmental or population factors and two are mutation that we have selected because they are located near the binding sites of Rac1. Rac1 has been modelled by Rosetta software and by i-Tasser web server. We have chosen i-Tasser based modelling because the Rac1 structure obtained was more closely resembling crystallography data. In silico model have been used as receptors for docking with a set of 20 morpholines. The results that have been obtained on SNPs shows that a single ligand can react very differently with a mutated structure. Our analysis shows that all mutations that have been considered change Rac1 conformation and increase the accessible surface of Rac1. Our analysis highlights the effect of two sources of genetic variability: single base variation and alternative splicing
Les edats d’«In memoriam»
No full textRemarks on "In memoriam", the largest poem by Gabriel Ferrater, nn the occasion of the 100th birth anniversary of the author
Impact of photovoltaic technology and feeder voltage level on the efficiency of façade building-integrated photovoltaic systems
No full textsponsorship: This project receives the support of the European Union, the European Regional Development Fund ERDF, Flanders Innovation & Entrepreneurship and the Province of Limburg. This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 751159. Special thanks goes to C. Sheehan from Bourns for providing the required inductor specifications. Patrizio Manganiello was previously with IMEC, Kapeldreef 75, Leuven, Belgium. (European Union, European Regional Development Fund ERDF, Flanders Innovation & Entrepreneurship, Province of Limburg, European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie|751159, Marie Curie Actions (MSCA)|751159)status: Published onlin
Modulatory effects of transcranial direct current stimulation (tDCS) on words and non-words reading task
No full textTITLE:
Modulatory effects of transcranial direct current stimulation (tDCS) on words and non-words reading task
AUTHOR(S):
Margherita Forgioneb, Patrizio Tressoldi b, Daniela Mapelli b, Paulo Sergio Boggio a
a Centro de Ciências Biológicas e da Saúde, Universidade Presbiteriana Mackenzie, Sao Paulo
b Dipartimento di Psicologia Generale, Università degli Studi di Padova, Italia
INTRODUCTION:
In recent past tDCS has gained much attention for rehabilitation purposes. Moreover, few studies have been conducted to investigate its efficacy on reading process. The aim of these studies was to investigate the role of left and right posterior temporal cortex in words and non-words reading process, through tDCS. We focused on the online task to see if it can influence the subjects’ performance. We hypothesized that the anodal stimulation would facilitate the task execution compared to sham and cathodal, in terms of reaction times and accuracy.
METHOD:
Twenty good readers were tested in 3 sessions (sham, anodal, cathodal), with different online task (text reading or music listening). tDCS was applied bilaterally over posterior temporal lobe, with a current of 1,5 mA, lasting for 20 minutes. Participants had to read aloud words or non-words, before and after stimulation. We recorded reaction times and accuracy for words and non-words, before and after stimulation.
RESULTS:
Regarding reaction times, we found significant effect of cathodal tDCS in short words reading task. Similarly, we also found an improvement in reading accuracy for non-words after tDCS. The online task doesn’t seem to affect the performance.
CONCLUSIONS:
We found a modulatory effect of cathodal tDCS in words reading task, suggesting a compensatory mechanisms of the right hemisphere. To verify this hypothesis, we are investigating different montages, involving left or right hemisphere. Further studies are necessary to understand the modulation effects of this technique, in addition with other methodologies, such as eye tracker, and with clinical sample, in particular dyslexics
In silico screening of Rac1 ligand specificity
No full textMicrotubule (MT) destabilization promotes the formation of actin stress fibers and enhances the contractility of cells. The actin cytoskeleton is bound to each junction and controls the integrity of each through actin remodeling and these junctions can be disassembled or assembled to either increase or decrease cellular permeability. Mediators, such as thrombin, stimulate their respective receptor on endothelial cells to initiate signaling that increases cytosolic Ca2+ and activates myosin light chain kinase (MLCK), as well as monomeric GTPases RhoA, Rac1, and Cdc42. Ca2+ activation of MLCK and RhoA disrupts junctions, whereas Rac1 and Cdc42 promote junctional assembly. In order to develop formal systems biology model of actin remodelling it is necessary to investigate the reciprocal interactions between Rac1 and Cdc42 by using experimental selective inhibition. We have screened, by docking analysis, a new class of compounds for Rac1 and/or Cdc42 inhibition, the Morpholinos, that could be used as alternative tool to switch off a gene
Toll-like receptor structural determinants: Variability analysis by digital signal processing methods
No full textIncerta glòria de Joan Sales: viatge entre les edicions i les traduccions
No full textJoan Sales’ Incerta glòria has a complicated textual history, as most of the literature
published in Spain during Franco’s dictatorship. As the novel deals with
the Spanish Civil War according to the losers’ viewpoint it was carefully read by
censors and of course censored. The editions of the novel published after the first
(1956) were continously changed and enlarged by the author who was, at the same
time, the publisher. After the author’s death in 1983, it was his wife who edited
the subsequent editions. It would be necessary therefore to have the text edited
and checked according to textual criticism. The article aims at reconstructing the
textual history of Incerta glòria from an external standpoint
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