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Indagine preliminare sulle caratteristiche microbiologiche e chimico-fisiche del Pecorino di Farindola
No full text
La concentrazione di acido malico quale indicatore per la valutazione qualitativa dei fieni
No full textMicrobiological and physicochemical characterization of naturally fermented Italian table olives
No full textQuinoxyfen 1995-2019 is the story's end? Evaluation of its adverse effects on head size and nervous system genes involved in synaptic maturation
No full text: In 2013 quinoxyfen (QXY) was included in the list of priority hazard pollutants of the European Water Framework Directive due to its toxicity to aquatic organisms. The use of products formulated containing QXY was banned from all commercials starting from 27th June of 2019. QXY is an organic pollutant with potential persistence, bioaccumulation and this must not lower attention to this pollutant. To date the effects of this compound on development are not completely clear, thus this research tries to elucidate the adverse effects of QXY on zebrafish development. The study aims to understand the toxicological effects of QXY using the zebrafish as in vivo model and performing toxicological and molecular investigations. Considering the FET test results two sublethal concentrations, 0.4 mg/L and 0.8 mg/L were chosen for subsequent analysis. The expression of the gad1b, cyp19a1b, shank3a, nrxn1a and c-fos genes, involved in the development of the nervous system and the regulation of synaptic transmission, were evaluated. To confirm the potential neurotoxic effects of the treatment on the development of the central nervous system, both a transgenic Tg(neuroD:gfp ia50) line was used for confocal microscopy and Orange Acridine was used on wild type larvae to assess the presence of neuronal apoptosis. The results showed sub-lethal alterations, particularly affecting craniofacial and brain development highlighting as QXY may represent a possible endocrine disruptor able to induce severe cartilage defects, small head and tremor phenotype in zebrafish larvae and a strong modulation of the selected genes
Microbiological and chemical profiles of naturally fermented table olives and brines from different Italian cultivars.
No full textSix naturally fermented Greek-style)table olives of cultivars Itrana, Peranzana, Cellina di Nardo`, Nocellara del Belice and Bella di Cerignola, as well as their corresponding brines, were studied by a combined strategy consisting of chemical, microbiological and molecular analyses. In particular, organic acids, sugars, polyphenols, fatty acids, biogenicamines and cultivable microbiota were detected bystandard methods. Moreover, tyramine and histamineproducing bacteria were evaluated by an originalapproach consisting of Reverse-Transcription (RT)-qPCR. At the end of the fermentation process,mesophilic lactobacilli and yeasts in brine representedthe dominating biota, ranging from 6.25 to 7.84 log CFU/ml and from 6.5 to 7.56 log CFU/ml, respectively. Enterobacteriaceae and pathogens were undetectable in all the samples. In general, table olive preparations differed in chemical composition. Inparticular, C16:0 and C18:2c9,12 concentrationsranged from 9.9 to 18.8 % and from 5.4 to 15.4 % of total fatty acids, respectively. The main fatty aciddetected was C18:1c9 while CLAc9, t11 was present only in traces. Polyphenol concentrations greatlydifferentiated the final product, depending on thecultivar. A low quantity of biogenic amines was foundin some samples and biogenic amines producingbacteria were rapidly detectable by RT-qPCR.[...
Effects of different diets on fatty acid profile of sheep milk and ricotta covered by the protected origin denomination “Ricotta Romana”
No full textQuinoxyfen 1995-2019 is the story's end? Evaluation of its adverse effects on head size and nervous system genes involved in synaptic maturation
Full text linkIn 2013 quinoxyfen (QXY) was included in the list of priority hazard pollutants of the European Water Framework Directive due to its toxicity to aquatic organisms. The use of products formulated containing QXY was banned from all commercials starting from 27th June of 2019. QXY is an organic pollutant with potential persistence, bioaccumulation and this must not lower attention to this pollutant. To date the effects of this compound on development are not completely clear, thus this research tries to elucidate the adverse effects of QXY on zebrafish development. The study aims to understand the toxicological effects of QXY using the zebrafish as in vivo model and performing toxicological and molecular investigations. Considering the FET test results two sublethal concentrations, 0.4 mg/L and 0.8 mg/L were chosen for subsequent analysis. The expression of the gad1b, cyp19a1b, shank3a, nrxn1a and c-fos genes, involved in the development of the nervous system and the regulation of synaptic transmission, were evaluated. To confirm the potential neurotoxic effects of the treatment on the development of the central nervous system, both a transgenic Tg(neuroD:gfp ia50) line was used for confocal microscopy and Orange Acridine was used on wild type larvae to assess the presence of neuronal apoptosis. The results showed sub-lethal alterations, particularly affecting craniofacial and brain development highlighting as QXY may represent a possible endocrine disruptor able to induce severe cartilage defects, small head and tremor phenotype in zebrafish larvae and a strong modulation of the selected gene
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