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Editorial for the Special Issue on “Multidisciplinary Insights on Bone Healing (Volume II)”
Full text linkAs the population ages and differences among sexes and age groups become more pronounced, the research on bone healing and damage mechanisms continues to advance, with evaluation conducted in both pre-clinical and clinical settings [...
Editorial for the Special Issue on “Multidisciplinary Insights on Bone Healing”
Full text linkAnimal and human bone damage can be considered differently according to a macro- or micro-level analysis [...
Biological variability of myoglobin in healthy elderly and younger subjects.
No full text“30° Congresso Nazionale della Società Italiana di Biochimica Clinica e Biologia Molecolare Clinica (SIBioC)”, Venezia 6-9 Ottobre 1998
Biological variability of myoglobin in women.
No full text“50th AACC Annual Meeting” Chicago, 2-6 Agosto 199
Altered free radicals/antioxidant balance in healthy old subjects – a marker of physiologic aging.
No full textComunic al: “28° Congresso Nazionale della Società Italiana di Biochimica Clinica e Biologia Molecolare Clinica”; Pesaro 8-11 Ottobre 1996
Altered oxidative stress in healthy old subjects.
No full textTo understand the magnitude of oxidative phenomena during senescence, we evaluated, as antioxidant, the activity of glutathione peroxidase (GSH-Px) in erythrocytes and plasma uric acid (UA) levels together with the malondialdehyde (MDA) levels in plasma, as an index of lipid peroxidation, in 46 apparently healthy elderly subjects (87±6 years old; mean±SD), and 49 young subjects (29±4 years old). The elderly subjects had lower erythrocyte GSH-Px activity (15.7±4.8 vs 20.2±7.0 U/g Hb, p<O.001; mean±SD) and plasma UA levels (192±46 vs 240±54 mmol/L, p<0.001); but higher MDA levels (5.3±0.8 vs 4.1±0.8 mmol/L, p<0.001) than the young subjects. Of additional in terest was the finding of a positive correlation between age and erythrocyte GSH-Px activity (r=0.74, p<0.001), and a negative correlation between age and plasma MDA levels (r=-0.83, p<0.001) in the elderly group. Although erythrocyte GSH-Px activity was significantly less in the elderly than in the young group, the oldest subjects showed the greatest erythrocyte GSH-Px activity and had lower MDA levels
Temperature-induced changes in lipid biomarkers and mycosporine-like amino acids in the psychrophilic dinoflagellate Peridinium aciculiferum
No full textLife at low temperature imposes many constraints linked to sustaining cellular functions. The cold-adapted freshwater dinoflagellate Peridinium aciculiferum has overcome these barriers, often causing blooms in winter but forming resting cysts in spring. Little is known of the biochemical changes that accompany this temperature-induced transformation from vegetative cells to resting cysts.
We investigated how the profiles of lipids and mycosporine-like amino acids (MAAs) vary with temperature in vegetative cells and resting cysts of P. aciculiferum. The freshwater dinoflagellate was grown at four temperatures (2.7–7.7 °C), simulating the seasonal changes from winter to spring that also induce the transition from cells to cysts. Biochemical profiles were established by liquid chromatography/mass spectrometry with the simultaneous detection of polar and non-polar compounds. Data were analysed by non-metric multidimensional scaling and ANOVA.
Over 100 species of galactolipids, betaine lipids, phospholipids and triacylglycerols (TAGs) were found, and many were strong biomarkers for specific temperatures and life stage. Variations in galactolipids, betaine lipids and phospholipids were unidirectional, as shown by an overall decrease in the unsaturation index with temperature. In contrast, changes in TAGs were specific to life stages: short-chain TAGs (cumulative acyl length of 44–52 carbon atoms) decreased in cysts with respect to vegetative cells, while long-chain TAGs (54–62) showed the opposite pattern. The concentration of MAAs decreased with increasing temperature. Final cell yield, a measure of population fitness, also decreased with increasing temperature, confirming the psychrophilic status of P. aciculiferum.
We report the first detailed biochemical profiles of vegetative cells and resting cysts for a dinoflagellate and show how small-scale temperature variations alter the biochemical make-up within and between life stages, thus contributing to our understanding of seasonal succession of specie
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