809 research outputs found
World War I record of service survey for Clarence R. Andrew, signed 29 September 1922
No full textQuestionnaire about Clarence Raymond Andrew's service in World War I, 1917-1919, signed by Andrew on 29 September 1922.Questionnaire originally part of a survey of Norwich University alumni; probably fire damaged in the Dewey Hall fire in 1925. Data from these questionnaires was used in a chapter of "Vermont in the world war, 1917-1919" by Harold P. Sheldon (1928). Transcription by Carina Berg. Transcriptions may be subject to error
The solution structure of a DNA·RNA duplex containing 5-propynyl U and C; comparison with 5-Me modifications
No full textThe addition of the propynyl group at the 5 position of pyrimidine nucleotides is highly stabilising. We have determined the thermodynamic stability of the DNA·RNA hybrid r(GAAGAGAAGC)·d(GCpUpUpCpUp CpUpUpC) where p is the propynyl group at the 5 position and compared it with that of the unmodified duplex and the effects of methyl substitutions. The incorporation of the propyne group at the 5 position gives rise to a very large stabilisation of the hybrid duplex compared with the analogous 5-Me modification. The duplexes have been characterised by gel electrophoresis and NMR spectroscopy, which indicate that methyl substitutions have a smaller influence on local and global conformation than the propynyl groups. The increased NMR spectral dispersion of the propyne-modified duplex allowed a larger number of experimental restraints to be measured. Restrained molecular dynamics in a fully solvated system showed that the propyne modification leads to substantial conformational rearrangements stabilising a more A-like structure. The propynyl groups occupy a large part of the major groove and make favourable van der Waals interactions with their nearest neighbours and the atoms of the rings. This enhanced overlap may account at least in part for the increased thermodynamic stability. Furthermore, the simulations show a spine of hydration in the major groove as well as in the minor groove involving the RNA hydroxyl groups
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ROTC students work a switchboard
No full textCadets Jerry R. Wooton, of Torrington Conn., a student of Worcester, and Don F. Zwiers, of North Judson, Ind., also a student of Worcester, are showing retired Col. Andrew H. Holt, head of Civil Engineering Department at Worcester, the correct proceedure for operation of the switchboard, SB-86
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Professor Holt with cadets
No full textProfessor Holt, having conference with cadets who attended Worcester Polytechnic Institute. (L to R) Davi A. Gilda, Newburgh, NY; George A. Robbins Jr., Leominster, Mass.; Professor Andrew H. Holt, Worcester, Mass.;John D. Minott, Asbury Park, NJ; John M. McHugh, Waterbury, Conn
World War I record of service survey for Hugo R. A. Anthony, signed 21 August 1922
No full textQuestionnaire about Hugo Robert Andrew Anthony's service in World War I, 1917-1919, signed by Anthony on 21 August 1922.Questionnaire originally part of a survey of Norwich University alumni; probably fire damaged in the Dewey Hall fire in 1925. Data from these questionnaires was used in a chapter of "Vermont in the world war, 1917-1919" by Harold P. Sheldon (1928). Transcription by Carina Berg. Transcriptions may be subject to error
Conformational properties and thermodynamics of the RNA duplex r(CGCAAAUUUGCG)2: comparison with the DNA analogue d(CGCAAATTTGCG)2.
No full textThe thermodynamic stability of nine dodecamers (four DNA and five RNA) of the same base composition has been compared by UV-melting. TheDeltaG of stabilisation were in the order: r(GACUGAUCAGUC)2>r(CGCAAATTTGCG)2 approximately r(CGCAUAUAUGCG)2>d(CGCAAATTTGCG)2 approximately r(CGCAAAUUUGCG)2>d(CGCATATATGCG)2 approximately d(GACTGATCAGTC)2>r(CGCUUUAAAGCG)2 approximately d(CGCTTTAAAGCG)2. Compared with the mixed sequences, both r(AAAUUU) and r(UUUAAA) are greatly destablising in RNA, whereas in DNA, d(TTTAAA) is destabilising but d(AAATTT) is stabilising, which has been attributed to the formation of a special B'structure involving large propeller twists of the A-T base pairs. The solution structure of the RNA dodecamer r(CGCAAAUUUGCG)2has been determined using NMR and restrained molecular dynamics calculations to assess the conformational reasons for its stability in comparison with d(CGCAAATTTGCG)2. The structures refined to a mean pairwise r.m.s.d. of 0.89+/-0.29 A. The nucleotide conformations are typical of the A family of structures. However, although the helix axis displacement is approximately 4.6 A into the major groove, the rise (3.0 A) and base inclination ( approximately 6 degrees ) are different from standard A form RNA. The extensive base-stacking found in the AAATTT tract of the DNA homologue that is largely responsible for the higher thermodynamic stability of the DNA duplex is reduced in the RNA structure, which may account for its low relative stability
M1 muscarinic receptor activation reduces the molecular pathology and slows the progression of prion-mediated neurodegenerative disease
No full textMany dementias are propagated through the spread of “prion-like” misfolded proteins. This includes prion diseases themselves (such as Creutzfeldt-Jakob disease) and Alzheimer’s disease (AD), for which no treatments are available to slow or stop progression. The M1 acetylcholine muscarinic receptor (M1 receptor) is abundant in the brain, and its activity promotes cognitive function in preclinical models and in patients with AD. Here, we investigated whether activation of the M1 receptor might slow the progression of neurodegeneration associated with prion-like misfolded protein in a mouse model of prion disease. Proteomic and transcriptomic analysis of the hippocampus revealed that this model had a molecular profile that was similar to that of human neurodegenerative diseases, including AD. Chronic enhancement of the activity of the M1 receptor with the positive allosteric modulator (PAM) VU0486846 reduced the abundance of prion-induced molecular markers of neuroinflammation and mitochondrial dysregulation in the hippocampus and normalized the abundance of those associated with neurotransmission, including synaptic and postsynaptic signaling components. PAM treatment of prion-infected mice prolonged survival and maintained cognitive function. Thus, allosteric activation of M1 receptors may reduce the severity of neurodegenerative diseases caused by the prion-like propagation of misfolded protein
Intersecting Axes: Narrative and Culture in Versions of the Lizzie Borden Story (A Performative Approach)
No full textThis thesis examines versions of the story of 32-year-old New Englander Lizzie Andrew Borden, famously accused of axe-murdering her stepmother Abby and father Andrew in 1892. Informed by narrative and feminist theories, INTERSECTING AXES draws upon interdisciplinary, contemporary re-workings of Judith Butler’s concept of “performativity” to explore the ways in which versions of the Lizzie Borden story negotiate such themes as repetition and difference, freedom and constraint, revision and reprisal, contingency and determinism, the specific and the universal. The project emphasizes and embraces the paradoxical sense in which interpretations are both enabled and constrained by the contextual situation of the interpreter and analyzes the relationship between individual versions and the cultural constructs they enact while purporting to describe.
Moving away from symptomatic reading and its psychoanalytic underpinnings to focus upon the interpretive frames by which our understandings of Lizzie Borden versions (and of narrative/cultural texts more broadly) are shaped, this project exposes the complex performative processes whereby meaning is created. The chapters of this thesis offer contextual readings of a short story by Mary E. Wilkins Freeman, a ballet by Agnes de Mille, a made-for-television by Paul Wendkos, and a short story by Angela Carter to argue for the theoretical, political, narratological, cultural, and interpretive benefits of approaching the relationship between texts and contexts through a uniquely contemporary concept of performativity, bringing a valuable new perspective to current debates about the intersection of narrative and culture
Hydration of the RNA duplex r(CGCAAAUUUGCG)2 determined by NMR.
No full textThe so-called spine of hydration in the minor groove of AnTn tracts in DNA is thought to stabilise the structure, and kinetically bound water detected in the minor groove of such DNA species by NMR has been attributed to a narrow minor groove [Liepinsh, E., Leupin, W. and Otting, G. (1994) Nucleic Acids Res. 22, 2249-2254]. We report here an NMR study of hydration of an RNA dodecamer which has a wide, shallow minor groove. Complete assignments of exchangeable protons, and a large number of non-exchangeable protons in r(CGCAAAUUUGCG)2 have been obtained. In addition, ribose C2'-OH resonances have been detected, which are probably involved in hydrogen bonds. Hydration at different sites in the dodecamer has been measured using ROESY and NOESY experiments at 11.75 and 14.1 T. Base protons in both the major and minor grooves are in contact with water, with effective correlation times for the interaction of approximately 0.5 ns, indicating weak hydration, in contrast to the hydration of adenine C2H in the homologous DNA sequence. NOEs to H1' in the minor groove are consistent with hydration water present that is not observed in the analogous DNA sequence. Hydration kinetics in nucleic acids may be determined by chemical factors such as hydrogen-bonding more than by simple conformational factors such as groove width
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