57 research outputs found
Psychotherapy and the Problems of Values
No full textThe author is an assistant professor in the Department of Psychiatry, School of Medicine, Case Western Reserve University, Cleveland and Director of Outpatient Psychiatry Day-Clinic, Cleveland Metropolitan General Hospital.
Dr. Dulin did his graduate studies at Loyola University, Chicago and was a post-doctoral fellow at Michael Reese Hospital, Chicago.
His article examines the absolutes and exceptions in the field of normalcy and reflects on the therapist\u27s function in helping his patient achieve his own value system
Studying genomic processes at the single-molecule level: introducing the tools and applications
No full textTo understand genomic processes such as transcription, translation or splicing, we need to be able to study their spatial and temporal organization at the molecular level. Single-molecule approaches provide this opportunity, allowing researchers to monitor molecular conformations, interactions or diffusion quantitatively and in real time in purified systems and in the context of the living cell. This Review introduces the types of application of single-molecule approaches that can enhance our understanding of genome function.Accepted Author ManuscriptBN/Nynke Dekker La
The Jogger
No full textBlack and white photograph of the Park Road Shopping Center sign at night Foundation Purchas
An Integrated Paleomagnetic and Diagenetic Study of the Womble Shale in the Benton Uplift, Arkansas
No full textThe objective of this project is to conduct an integrated paleomagnetic and diagenetic study of the Ordovician Womble Shale in Mt. Ida, Arkansas, in order to test if diagenetic events can be identified and dated with petrographic and paleomagnetic techniques. Significant hydrothermal alteration has been identified and dated in western Arkansas and the paleomagnetic analysis will test if a magnetization of similar age that was caused by hydrothermal fluids can be identified. Samples were collected from a Womble Shale outcrop, located west of Mt. Ida, Arkansas, which are composed of shale alternating with calcareous sandstone lenses that contain numerous veins filled with quartz and calcite. The samples were characterized based on thin section petrography and scanning electron microscopy (SEM), and a paragenetic sequence was determined. In addition to acquiring paleomagnetic data to determine the timing, anisotropy of magnetic susceptibility (AMS) data was collected to examine the petrofabrics and identify alteration.
The calcareous sandstones contain a complicated diagenetic history which includes precipitation of authigenic phases during multiple fluid events and deformation. The calcite and quartz veins, both vertical and horizontal, are interpreted to be hydrothermal and syntectonic in origin. Hydrothermal minerals including xenotime, monazite, biotite, and sphalerite are identified within veins and in the matrix as are hydrocarbons. Stylolites containing mics are present in the matrix and in larger veins. Fibrous clay bands composed predominantly of muscovite and illite with hematite staining are also present in the matrix. Diagenesis within the Womble Shale indicates a unit influenced by hydrothermal fluids, most likely in several episodes, low-grade metamorphism, and deformation.
AMS data suggests that the petrofabric of the Womble Shale is a composite fabric with influences from burial, tectonic, and diagenetic processes. Alternating field (AF) demagnetization identified a post tilting thermoviscous remanent magnetization (TVRM) held in magnetite with a Late Mississippian pole. The demagnetization paths are curved which suggest contamination with a modern viscous magnetization and the pole age is not considered reliable. A magnetic component removed during thermal demagnetization after AF treatment reveals a post tilting characteristic remnant magnetization (ChRM) held in hematite with a Late Pennsylvanian pole position. The ChRM is interpreted as a thermochemical remnant magnetization (TCRM) caused by precipitation of hematite during hydrothermal alteration. The petrographic and paleomagnetic results suggest the acquisition of a syntectonic hematite magnetization in the Late Pennsylvanian probably coincided with hydrothermal alteration. This contrasts with other studies which report post orogenic hydrothermal activity in the Ouachitas (e.g., Shelton et al., 1986)
On Impact of Helical Structures on Stabilization of Swirling Flames with Vortex Breakdown
No full textWe report on a study of the impact of coherent helical vortex structures on the shape of the reaction zone and heat release in swirling methane/air flames in regimes with a vortex breakdown. Three kinds of atmospheric flames are considered, viz., fuel-lean and fuel-rich premixed flames and a partially premixed fuel-rich lifted flame. Based on the measurements of the velocity fields by a stereo PIV in combination with the OH PLIF and HCHO PLIF, the impact of the coherent flow structures on large-scale corrugations of the reaction zone is evaluated. Helical vortex structures, detected in both the non-reacting and reacting high-swirl flows by using proper orthogonal decomposition, are found to promote combustion both in the lean premixed and fuel-rich partially premixed flames. In the first case, based on the phase-averaged intensity of the HCHO×OH signal and the location of the helical vortex structure in the inner mixing layer, it is concluded that the vortex locally increases the heat release rate by enlarging the flame front and enhancing the mass exchange between the combustion products inside the recirculation zone and the fresh gases. The events of the local flame extinctions are detected in the instantaneous PLIF snapshots for the lean mixture, but they do not cause extinction of the entire flame or a blow-off. In case of the lifted flame, the outer helical vortex structure promotes combustion by locally intensifying the mass exchange between the fuel-rich jet with the surrounding air.Accepted Author ManuscriptChemE/Transport Phenomen
Untangling reaction pathways through modern approaches to high-throughput single-molecule force-spectroscopy experiments
No full textSingle-molecule experiments provide a unique means for real-time observation of the activity of individual biomolecular machines. Through such techniques, insights into the mechanics of for example, polymerases, helicases, and packaging motors have been gleaned. Here we describe the recent advances in single-molecule force spectroscopy instrumentation that have facilitated high-throughput acquisition at high spatiotemporal resolution. The large datasets attained by such methods can capture rare but important events, and contain information regarding stochastic behaviors covering many orders of magnitude in time. We further discuss analysis of such data sets, and with a special focus on the pause states described in the general literature on RNA polymerase pausing we compare and contrast the signatures of different reaction pathways.BN/Nynke Dekker LabBN/Martin Depken La
Mechanism of assembly of an elongation-competent SARS-CoV-2 replication transcription complex
No full textGreen Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.BN/Martin Depken LabBN/BionanoscienceBN/Nynke Dekker La
The components necessary for successful information sharing
No full textCHDS State/LocalThe need for information sharing is a growing concern for many agencies in the homeland security field. As communities struggle to prepare for and respond to major incidents, information sharing between agencies is vital. Fusion centers developed around the law enforcement community, which has led to an information-sharing network that is exclusive. Non-law enforcement agencies such as Fire, EMS and Public Health that are charged with preparing for and responding to major incidents need the exchange of information as well. This thesis identifies several components that affect knowledge transfer. The human elements of Relationships, Trust, Megacommunities, Governance, and Leadership form the basis for successful information sharing networks. On this base, the technical components of the information-sharing network such as Standard Operating Procedures, Technology Standards, and Interoperability can be built.Deputy Chief, / UASI POC, Charlotte (NC) Fire Department author (civilian).http://archive.org/details/thecomponentsnec10945488
Signatures of Nucleotide Analog Incorporation by an RNA-Dependent RNA Polymerase Revealed Using High-Throughput Magnetic Tweezers
No full textSummary: RNA viruses pose a threat to public health that is exacerbated by the dearth of antiviral therapeutics. The RNA-dependent RNA polymerase (RdRp) holds promise as a broad-spectrum, therapeutic target because of the conserved nature of the nucleotide-substrate-binding and catalytic sites. Conventional, quantitative, kinetic analysis of antiviral ribonucleotides monitors one or a few incorporation events. Here, we use a high-throughput magnetic tweezers platform to monitor the elongation dynamics of a prototypical RdRp over thousands of nucleotide-addition cycles in the absence and presence of a suite of nucleotide analog inhibitors. We observe multiple RdRp-RNA elongation complexes; only a subset of which are competent for analog utilization. Incorporation of a pyrazine-carboxamide nucleotide analog, T-1106, leads to RdRp backtracking. This analysis reveals a mechanism of action for this antiviral ribonucleotide that is corroborated by cellular studies. We propose that induced backtracking represents a distinct mechanistic class of antiviral ribonucleotides. : Dulin et al. find that a prototypical RNA-dependent RNA polymerase (RdRp) visits several states during nucleotide synthesis, of which only one incorporates nucleotide analogs with therapeutic potential. Different analogs exhibit distinct kinetic signatures, with an analog thought to induce chain termination actually promoting RdRp backtracking. Keywords: RNA virus, RNA polymerase, magnetic tweezers, inhibitor, nucleoside analog, backtracking, pyrazine carboxamide, T-1106, T-70
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