50 research outputs found
Complete genome sequence of rhizobium sp. strain 11515TR, isolated from tomato rhizosphere in the Philippines
No full text© 2018 Montecillo et al Rhizobium sp. strain 11515TR was isolated from the rhizosphere of tomato in Laguna, Philippines. The 7.07-Mb complete genome comprises three replicons, one chromosome, and two plasmids, with a GC content of 59.4% and 6,720 protein-coding genes. The genome encodes gene clusters supporting rhizosphere processes, plant symbiosis, and secondary bioactive metabolites
Near-complete genome sequence of ralstonia solanacearum T523, a phylotype i tomato phytopathogen isolated from the Philippines
No full textCopyright © 2018 Montecillo et al. Ralstonia solanacearum strain T523 is the major phytopathogen causing tomato bacterial wilt in the Philippines. Here, we report the complete chromosome and draft megaplasmid genomes with predicted gene inventories supporting rhizosphere processes, extensive plant virulence effectors, and the production of bioactive signaling metabolites, such as ralstonin, micacocidin, and homoserine lactone
The dependence of the characteristics of THz current oscillations on the quantum-well width in resonant tunneling diode
No full textDraft genome sequence of streptomyces sp. Isolate H28 from the meycauayan River, Philippines
No full textIn this paper, we report the draft genome sequence of Streptomyces sp. isolate H28, isolated from sediments of the Meycauayan River in the Philippines. This species exhibits production of melanin as well as the ability to utilize and degrade both high-density polyethylene (HDPE) and low-density polyethylene (LDPE)
Coding-Complete Genome Sequence of an African Swine Fever Virus from an Outbreak in 2021 among Domestic Pigs in Pangasinan, Philippines
No full textWe report a coding-complete genome sequence of an African swine fever virus from an outbreak in 2021 among domestic pigs in Pangasinan, Philippines using Oxford Nanopore Technologies minION. The linear genome assembly is a single contig with 192,377 bp.</p
Bacterial diversity of bat guano from Cabalyorisa Cave, Mabini, Pangasinan, Philippines: A first report on the metagenome of Philippine bat guano
No full text© 2018 P. De Leon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Bats are highly diverse and ecologically valuable mammals. They serve as host to bacteria, viruses and fungi that are either beneficial or harmful to its colony as well as to other groups of cave organisms. The bacterial diversity of two bat guano samples, C1 and C2, from Cabalyorisa Cave, Mabini, Pangasinan, Philippines were investigated using 16S rRNA gene amplicon sequencing. V3-V4 hypervariable regions were amplified and then sequenced using Illumina MiSeq 250 PE system. Reads were processed using Mothur and QIIME pipelines and assigned 12,345 OTUs for C1 and 5,408 OTUs for C2. The most dominant OTUs in C1 belong to the Proteobacteria (61.7%), Actinobacteria (19.4%), Bacteroidetes (4.2%), Firmicutes (2.7%), Chloroflexi (2.5%), candidate phylum TM7 (2.3%) and Planctomycetes (1.9%) while Proteobacteria (61.7%) and Actinobacteria (34.9%) dominated C2. Large proportion of sequence reads mainly associated with unclassified bacteria indicated possible occurrence of novel bacteria in both samples. XRF spectrophotometric analyses of C1 and C2 guano revealed significant differences in the composition of both major and trace elements. C1 guano recorded high levels of Si, Fe, Mg, Al, Mn, Ti and Cu while C2 samples registered high concentrations of Ca, P, S, Zn and Cr. Community structure of the samples were compared with other published community profiling studies from Finland (SRR868695), Meghalaya, Northeast India (SRR1793374) and Maharashtra State, India (CGS). Core microbiome among samples were determined for comparison. Variations were observed among previously studied guano samples and the Cabalyorisa Cave samples were attributed to either bat sources or age of the guano. This is the first study on bacterial diversity of guano in the Philippines through high-throughput sequencing
Copper sulfate-embedded and copper oxide-embedded filter paper and their antimicrobial properties
No full textCopper sulfate-embedded and copper oxide-embedded filter paper and their antimicrobial properties
No full text© 2017 Elsevier B.V. In this study, copper sulfate was successfully embedded in filter paper through soaking and copper oxide was successfully embedded in filter paper via in situ technique using the copper sulfate-embedded paper as the starting material. Using color analysis, the filter paper soaked with copper sulfate solution was observed to change color from white to blue, indicating the presence of embedded copper sulfate salts. Furthermore, upon soaking this filter paper with sodium hydroxide solution, the color changed from blue to brown, indicating the presence of copper oxide. X-ray diffraction (XRD) spectroscopy confirms the observed results of the color analysis. Scanning electron microscopy (SEM) and electron dispersive x-ray (EDX) spectroscopy were used to determine the surface morphology and elemental composition of the samples, respectively. The antimicrobial property of the filter paper with copper-based particles was tested against Gram-negative bacterium (Escherichia coli) and Gram-positive bacterium (Staphylococcus aureus), and two species of fungi (Candida albicans and Aspergillus niger) using disc diffusion method. The reactivity of the copper sulfate-embedded filter paper against E. coli was found to be severe, whereas that against S. aureus was moderate. The reactivity of the samples against the two fungi was mild, having a relatively small zone of inhibition. For the copper oxide-embedded paper, the reactivity of the paper to both bacteria was moderate, whereas it shows no reactivity to C. albicans and only a mild reactivity to A. niger. After 60 min of contact with Escherichia coli, both the copper sulfate-embedded and copper oxide-embedded filter papers reduced the amount of colony forming unit to more than 99.9%
Effect of organic fertilizer Amina P on the yield of pineapple (Ananas comosus L.) Merr. and Soil microbial population
No full textOrganic fertilizer Amina P significantly increased the yield of pineapple (Ananas comosus L.) Merr. 18 months after planting (MAP). However, vegetative growth parameters measured 8 and 10 MAP were not significantly affected by Amina P and inorganic fertilizer applications. The weight and length of the fruits harvested from plots amended with the Amina P and inorganic fertilizer were higher than those from the unamended control. In contrast, the pineapple yield from the Amina P and inorganic fertilized soils were not significantly different, indicating the potential of Amina P as an alternative to inorganic fertilizer. Analysis of the soil bacterial population by high-throughput sequencing showed that Amina P fertilization was not detrimental to the naturally-occurring soil bacterial community
