81 research outputs found
Irina Albăstroiu, Calcedonia Enache, Andrei Cepoi, Adrian Istrate and Teodora Liliana Andrei
No full textIoT (Internet of Things) is a concept that defines a world in which all objects are connected
to each other via the Internet. The ability of smart devices to connect, communicate and
transfer data has enabled the innovation and development of various solutions for industry,
business organizations and final consumers. In this article, we have chosen to discuss the
solutions related to smart homes.
Thus, our paper presents, in the first part, the conceptual delimitations regarding IoT, areas
of application, and characteristics of the solutions dedicated to smart homes, showing that
adoption of IoT-based smart home solutions has been too little addressed in the literature.
Most of the work in the field insists on the technical aspects and only subsidiarily deals with
the issues regarding the level of understanding of the IoT smart home concept by potential or
actual users and the degree of adoption and use of these solutions. In order to cover these
gaps identified in the literature, we presented, in the second part of the paper, the
methodology and results of an exploratory research, conducted on a sample of 471 persons,
which allowed us to identify the socio-demographic characteristics of the respondents (users
of IoT solutions), level of understanding the IoT concept, degree of the adoption and, also,
the associated benefits and challenges, from the perspective of the Romanian users. We also
developed a binary logistic regression model, for in-depth analysis of the results and for
correlating the findings of our research with those of other studie
2′β-Fluoro-Tricyclo Nucleic Acids (2′F-tc-ANA): Thermal Duplex Stability, Structural Studies, and RNase H Activation
No full textWe describe the synthesis, thermal stability, structural and RNase H activation properties of 2′β-fluoro-tricyclo nucleic acids (2′F-tc-ANA). Three 2′F-tc-ANA nucleosides (T, 5MeC and A) were synthesized starting from a previously described fluorinated tricyclo sugar intermediate. NMR analysis and quantum mechanical calculations indicate that 2′F-tc-ANA nucleosides prefer sugar conformations in the East and South regions of the pseudorotational cycle. UV-melting experiments revealed that non-consecutive insertions of 2′F-tc-ANA units in DNA reduce the affinity to DNA and RNA complements. However, an oligonucleotide with five contiguous 2′F-tc-ANA-T insertions exhibits increased affinity to complementary RNA. Moreover, a fully modified 10-mer 2′F-tc-ANA oligonucleotide paired to both DNA (+1.6 °C/mod) and RNA (+2.5 °C/mod) with significantly higher affinity compared to corresponding unmodified DNA, and similar affinity compared to corresponding tc-DNA. In addition, CD spectroscopy and molecular dynamics simulations indicate that the conformation of the 2′F-tc-ANA/RNA duplex is similar to that of a DNA/RNA duplex. Moreover, in some sequence contexts, 2′F-tc-ANA promotes RNase H-mediated cleavage of a complementary RNA strand. Taken together, 2′F-tc-ANA represents a nucleic acid analogue that offers the advantage of high RNA affinity while maintaining the ability to activate RNase H, and can be considered a prospective candidate for gene silencing applications
6’-Fluoro[4.3.0]bicyclo nucleic acid: synthesis, biophysical properties and molecular dynamics simulations
No full textHere we report on the synthesis, biophysical properties and molecular modeling of oligonucleotides containing unsaturated 6’-fluoro[4.3.0]bicyclo nucleotides (6’F-bc4,3-DNA). Two 6’F-bc4,3 phosphoramidite building blocks (T and C) were synthesized starting from a previously described [3.3.0]bicyclic sugar. The conversion of this sugar to a gem-difluorinated tricyclic intermediate via difluorocarbene addition followed either by a NIS-mediated or Vorbrüggen nucleosidation yielded in both cases the β-tricyclic nucleoside as major anomer. Subsequent desilylation and cyclopropane ring opening of these tricyclic intermediates afforded the unsaturated 6’F-bc4,3 nucleosides. The successful incorporation of the corresponding phosphoramidite building blocks into oligonucleotides was achieved with tert-butyl hydroperoxide as oxidation agent. Thermal melting experiments of the modified duplexes disclosed a destabilizing effect versus DNA and RNA complements, but with a lesser degree of destabilization versus complementary DNA (ΔTm/mod = −1.5 to −3.7 °C). Molecular dynamics simulation on the nucleoside and oligonucleotide level revealed the preference of the C1’-exo/C2’-endo alignment of the furanose ring. Moreover, the simulation of duplexes with complementary RNA disclosed a DNA/RNA-type duplex structure suggesting that this modification might be a substrate for RNase H
NMR solution structure of tricyclo-DNA containing duplexes: insight into enhanced thermal stability and nuclease resistance
No full textTc-DNA is a conformationally constrained oligonucleotide analogue which shows significant increase in thermal stability when hybridized with RNA, DNA or tc-DNA. Remarkably, recent studies revealed that tc-DNA antisense oligonucleotides (AO) hold great promise for the treatment of Duchenne muscular dystrophy and spinal muscular atrophy. To date, no high-resolution structural data is available for fully modified tc-DNA duplexes and little is known about the origins of their enhanced thermal stability. Here, we report the structures of a fully modified tc-DNA oligonucleotide paired with either complementary RNA, DNA or tc-DNA. All three investigated duplexes maintain a right-handed helical structure with Watson-Crick base pairing and overall geometry intermediate between A- and B-type, but closer to A-type structures. All sugars of the tc-DNA and RNA residues adopt a North conformation whereas the DNA deoxyribose are found in a South-East-North conformation equilibrium. The conformation of the tc-DNA strand in the three determined structures is nearly identical and despite the different nature and local geometry of the complementary strand, the overall structures of the examined duplexes are very similar suggesting that the tc-DNA strand dominates the duplex structure
The study of phenotypic characters of indigenous varieties of vines using mathematical methods based on ampelometry
No full textAnaliza cluster la soiurile de viță de vie provenite din Coarnă Neagră
No full textThe Coarnă neagră grapevine variety was used as maternal genitor in the
creation of some new grapevine varieties. Of these varieties, the author have chosen
Coarnă neagră selecționată, Azur, Milcov, Gelu, Ozana and Mara with which, on
the basis of Cluster analysis will determine the phenotypical similarity among the
Coarnă neagră varieties genitor and its descendants, with a view to establish the
polyphyletic groupings among the biological material under analysis
Analiza cluster la soiurile de viță de vie provenite din Coarnă Neagră
No full textThe Coarnă neagră grapevine variety was used as maternal genitor in the
creation of some new grapevine varieties. Of these varieties, the author have chosen
Coarnă neagră selecționată, Azur, Milcov, Gelu, Ozana and Mara with which, on
the basis of Cluster analysis will determine the phenotypical similarity among the
Coarnă neagră varieties genitor and its descendants, with a view to establish the
polyphyletic groupings among the biological material under analysis
Rezultate privind aplicarea analizei fractale la soiurile de viţă de vie descendente din Băbească Neagră
No full textIn order to obtain new vine varieties, the vine breeders were focused
on using the ancestral and productive varieties that are well adapted to the
ecopedoclimatic conditions of our vineyards. Thus, for the
Băbeascăneagrăvariety case it resulted the following romanian varieties:
Băbeascăgri, Codană, Arcaş, Balada, Cristina and Mamaia to which was
applied the method of fractal analysis to determine the degree of similarity. It
resulted that the fractal dimension closest to the parent variety was at
Mamamia variety of 1.1254, being the largest value, and the farthest was of
1.0067 at Balada variety
Author affiliation countries.
No full textChoropleth map shows author affiliation countries based on matches to ISO-3166-1 country names or Alpha-3 codes. Color is scaled to the number of times that country’s name appeared in the author affiliations across all articles in the inventory. Figure was created using the R ggplot2 package which obtains map data from Natural Earth [71], which is in the public domain. (TIF)</p
Aplicarea analizei fractale la sortogrupul Coarnă
No full textCoarnă neagră and Coarnă albă vine varieties were used as maternal
genitor in order to obtain new romanian vine creations. Out of these were
included in the study Coarnă neagră selecţionată, Azur, Milcov, Gelu, Ozana
and Mara varieties within Coarnă neagră sortogroup. After applying the fractal
analysis, it was determined the differences between the parent variety and its
descendants. It resulted the fact that the fractal dimension closest to Coarnă
neagră variety it is owned by Coarnă neagră selecţionată variety (1.0389) and
the farthest it is owned by Ozana variety (1.1254). For the Coarnă albă
sortogroup, the value closest to the genitor was 1.0855, at Mioriţa variety, and
the furthest was at Muscat Timpuriu de Bucureşti variety of 1.1124
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