48,327 research outputs found
In vivo and in vitro evaluation of phytic acid as topical antioxidant
No full textThe use of locally applied antioxidants has been shown as an effective approach for the skin photo-protection. In a previous investigation we have demonstrated that phytic acid (PA), a component of most cereal grains and seeds, is able to reduce the erythemal response to UV-exposure when applied 30 minutes before UV-radiation. PA was used at 0.5 % concentration in a O/W emulsion containing also inositol at 2.0 % concentration. Before the test and 24 and 48 h after irradiation the extent of the reaction was evaluated with visual scoring as well as with instrumental measurement of skin redness,TEWL and hydration. In this study we have evaluated the ability of PA to protect human keratinocytes cultures against UV exposure. PA extracted from the rice bran was used at 100 μM. The experiments were performed also on ascorbic acid
at the same concentration. Human HaCaT keratinocytes were grown in 3,5 cm2 plates containing complete medium (DMEM with 10% fetal calf serum). Thirty minutes before UV radiation, medium was removed and replaced by PBS with or without the antioxidants. The light source was a 150-Watt xenon arc solar simulator. Series of cultures were irradiated with scalar UV-doses ranging from 11,56 to 92,48 mJ/cm2. After irradiation PBS solution with or without antioxidants was removed and replaced with fresh medium. The antioxidant activity was evaluated by MTT
assay after 24 hrs. The solutions containing the active ingredients demonstrated a protective ability stronger than that of the controls. Particularly PA showed an ability to protect the keratinocytes against UV damage more pronounced than vitamin C. Our investigation has proved
that phytic acid is able to protect human keratinocytes against UV radiation. This means that epidermal cells are involved in the PA-mediated photoprotection in vivo and represents a fur-ther support for the use of PA as topical photoprotective agent
UVA light stimulates the production of cathepsin G and elastase-like enzymes by dermal fibroblasts: a possible contribution to the remodeling of elastotic areas in sun-damaged skin
No full textSolar elastosis is characterized by accumulation of large amounts of material staining similarly to elastin in the dermis. The nature of this material and the process responsible for its accumulation are still unknown. Elastolytic proteases have important functions in the catabolism of the interstitial matrix and can also generate, by the digestion of the interstitial proteins, soluble peptides which can induce collagen and elastin synthesis and deposition. We investigated whether (i) elastolytic enzymes can be detected in samples from sun-exposed and non-exposed skin, and (ii) ultraviolet (UV) rays influence the production of elastolytic activities in cultured dermal fibroblasts. Immunoelectron microscopy showed a positive reaction for neutrophil elastase and cathepsin G in fibroblast-like cells from specimens of sun-exposed areas. Little or no reaction was found in biopsies of sun-protected skin. Fibroblast cultures from sun-exposed skin expressed higher levels of hydrolytic activity against synthetic substrates of elastases and cathepsin G than those obtained from sun-protected areas. Irradiation with UVA strongly stimulated the production of these activities in fibroblasts from sun-protected sites. No significant change was detected in parallel sets of cultures after UVB irradiation. Inhibition experiments indicated that the elastase-like activity expressed by fibroblasts can be attributed to at least two enzymes
Spring, it’s time to ROSC
No full textOut‐hospital cardiac arrest (OHCA) is a multi‐factor disease. Many studies have correlated OHCA with a patient’s lifestyle; unfortunately, less evidence highlights the correlation with mete‐ orological factors. Methods: Analysis of 23959 OHCA rescue performed by the emergency medical system (EMS) of Lombardy Region, the most Italian populated region, in 2018 and 2019, the pre‐pandemic era through a retrospective observational cohort study. The aim of the study consists on evaluating the probabil‐ ity of Return Of Spontaneous Circulation (ROSC) during months to highlight potential seasonal impact in ROSC achievement. In March and April, we highlight an increase of ROSC (OR: 1.20 95% CI 1.04‐1.31; p < 0.001) compared to other months. Dur‐ ing March and April, we highlight an increase of public access defibrillation (PAD) (3.5% vs 2.5%; p < 0.001), and a reduction of overage time of first vehicle on scene (11.5 vs 11.8; p < 0.001) and age of patient (73.5 vs 74.2; p < 0.01). Finally, we highlight a slight reduction of cancer patient (1.6% vs 1.1%; p = 0.01). We didn’t register significant differences in the other variables ana‐ lyzed as: onset place, sex, rescue team and the patient’s death before the rescue arrive. We highlight a difference in ROSC prob‐ ability during the first month of spring. We register few differences in patient characteristics and EMS rescue, though just PAD use and age clinically impact OHCA patients. In this study, we are unable to fully understand the modification of the probability of ROSC in these months. Even though four variables have a statisti‐ cally significant difference, they can’t fully explain this modifica‐ tion. Different variables like meteorological and seasonal factor must be considered. We propose more research on this item
Memoria di Lavoro e Funzione Narrativa in un campione di adolescenti con e senza esperienze traumatiche
No full textA new device for objective assessment of skin type in Caucasians by violet light reflectance
No full textIn the present study, we tested a new device called skin phototype diagnosis (SPD) built for the purpose of objectively determining skin phototype. We compared its performance with that of phototype determinations according to Fitzpatrick method and on tristimulus colorimetry (Minolta CR-200). Our population consisted of 100 subjects of Caucasian race (60 female, 40 male; mean age 33 years). Skin colour was measured with both devices (SPD and Minolta CR-200) on the medial surface of the arm (constitutional skin colour). Our study showed that the SPD gave a better representation of Fitzpatrick phototype, showing 89% concordance (evaluated by classification matrix) as against the 71% concordance of the L(*)a(*)b(*) and Yxy colorimetric systems. The present results are important because evaluation of phototype with the SPD device is easy, fast, objective and reliable. Moreover, this instrument has potential applications in cosmetology and in photodermatology
Immuno-histopathologic changes in the gastrointestinal mucosa in patients with nickel contact allergy
No full textThoracocervicofacial purpura as the single manifestation of epileptic seizure
No full textA 44 year old woman who underwent ovariectomy due to bilateral ovarian cysts at the age of 29 followed by hormone replacement therapy for several years, was admitted to hospital after a generalised grand mal seizure. The patient had no history of epilepsy. Neurological examination on admission was negative; however, purpura of the thoracocervicofacial region was seen (figure). Routine blood chemistry (platelet count on FBC) and chest radiography were normal. Cranial CT and MRI did not show any parenchymal pathology. An EEG trace was within normal limits.
Purpura was described in association with generalised seizures by Bychowski.1 Guillaume proposed that this type of purpura was of mechanical origin5, due to intense contraction of the chest and abdominal muscles with the glottis closed during the tonic-clonic phase, as in the Valsalva manoeuvre
Plasma levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and N-terminal fragment of pro-ANP in heart failure and in controls
No full textSignificato fisiopatologico ed utilità clinica dei metodi di dosaggio dei peptidi natriuretici cardiaci
No full textRole of extracorporeal photochemotherapy alone and in combination with interferon alfa in the treatment of cutaneous T-cell lymphoma
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