19 research outputs found
Mechanical and electrical development of construction mobile manipulator
No full textWith the development of technology in 21st century, Robotics is increasingly getting closer to our daily life. In this report, a new robotics application in contraction field is illustrated. The robot is designed to finish the specified construction works which provided a high economical solution for a sustainable development for the future.
In this report, author will introduce a full proposal of mechanical design for making the real size robot, and a scaling down robot is also carried out to simulate the electrical and logical flow, consequently, increased feasibility of the robot.Bachelor of Engineering (Mechanical Engineering
Hemispheric asymmetries in metaphor processing
No full textWhat is the role of the right hemisphere in processing metaphors? One theory suggests that metaphor processing in the RH is part of amore general function of the RH, coarse semantic coding (Beeman,1998). The LH mediates semantic relationships between words havinghigh semantic feature overlap, whereas the RH activates a broadersemantic network of more distantly related words.College Honors
Multilingual twitter corpus and baselines for evaluating demographic bias in hate speech recognition
No full textExisting research on fairness evaluation of document classification models mainly uses synthetic monolingual data without ground truth for author demographic attributes. In this work, we assemble and publish a multilingual Twitter corpus for the task of hate speech detection with inferred four author demographic factors: age, country, gender and race/ethnicity. The corpus covers five languages: English, Italian, Polish, Portuguese and Spanish. We evaluate the inferred demographic labels with a crowdsourcing platform, Figure Eight. To examine factors that can cause biases, we take an empirical analysis of demographic predictability on the English corpus. We measure the performance of four popular document classifiers and evaluate the fairness and bias of the baseline classifiers on the author-level demographic attributes
Performance of Coherent BPSK Systems using Phase Compensation and Diversity Techniques
No full textThe performance of free-space optical (FSO) communication suffers from atmospheric turbulence, which mainly results in scintillation and phase fluctuations to optical signal. In this paper, we study the bit error rate (BER) performance of coherent FSO system employing phase compensation and spatial diversity. Based on weak fluctuation theory, the amplitude fading and phase fluctuation induced by atmospheric turbulence are assumed to follow log-normal model and Gaussian distribution, respectively. Distinct from previous works, a new closed-form expression for signal fading at the receiver is derived. Then the impact of various compensating parameters is examined on the BER performance of the system, including the normalized receiver aperture diameter and the number of phase compensation modes. The optimal compensation parameters are found showing that the FSO coherent system can obtain the minimum BER. Furthermore, we explore the potential of spatial diversity, particularly receiver diversity to further enhance the system performance. Numerical results show that the addition of phase compensation and diversity techniques can largely reduce the impact of atmospheric turbulence.Engineering, Electrical & ElectronicTelecommunicationsEICPCI-S(ISTP)
Performance of phase compensated coherent free space optical communications through non-Kolmogorov turbulence
No full textThe bit-error-rate (BER) performance of coherent free-space optical (FSO) links employing phase compensation techniques is investigated in weak non-Kolmogorov turbulence. Assuming that the amplitude fading and phase fluctuation follow lognormal model and Gaussian distribution respectively and using the expression of non-Kolmogorov turbulence in terms of Zernike polynomials, the signal-to-noise ratio (SNR) at the coherent receiver is analyzed and as a special case, a new closed-form expression using chi-square distribution is obtained. Thus, the influence of different compensation modes and normalized receiver diameter on BER performance is evaluated and an optimum normalized receiver diameter is suggested to achieve the minimum BER. Moreover, the impact of outer scale L(0) and the exponent value alpha in non-Kolmogorov spectrum is studied with the optimum diameter, which reveals that the BER has an obvious decrease with larger values of L(0) and alpha. (C) 2010 Elsevier B.V. All rights reserved.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000287628500002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701OpticsSCI(E)EI7ARTICLE61491-149528
Structural studies of CRISPR-associated proteins
No full textClustered regularly interspaced short palindromic repeats (CRISPRs) act to prevent viral infection and horizontal gene transfer in prokaryotes. The genomic CRISPR array contains short sequences (“spacers”) that are derived from foreign genetic elements. The CRISPR array is transcribed and processed into CRISPR RNAs (crRNAs) used in the sequence-specific degradation of foreign nucleic acids. This process is called interference and is mediated by CRISPR-associated (Cas) proteins.
This thesis has focused on the structural and functional characterisation of four Cas proteins from the CRISPR/Cas system of Sulfolobus solfataricus. The crystal structure of Cmr7 (Sso1725), a Sulfolobales-specific subunit of the ssRNA-degrading CMR complex, allowed for the identification of a putative protein-binding site, though no specific function could be ascribed to the protein. Cas6 (Sso1437) is the enzyme responsible for crRNA maturation and the characterisation of this protein allowed for the molecular rationalisation of its atypical RNA cleavage mechanism. Csa5 and Cas8a2 are subunits of the aCascade complex that targets dsDNA. Csa5 (Sso1398) was shown to have a putative role in R-loop stabilisation during interference while the role of Cas8a2 (Sso1401) was not determined. The structures of these two proteins were used to define relationships between the subunits of interference complexes from various CRISPR/Cas systems.
A second aspect of this work has been the expression and purification of eukaryotic ion channels for structural studies. The acid sensing ion channel (ASIC) and FMRFamide-gated sodium channel (FaNaC) are gated ion channels with unknown mechanisms of channel activation. These ion channels must be expressed in eukaryotic systems and so human embryonic kidney (HEK) cells and baculovirus-insect cell expression systems were developed to express ASIC and FaNaC constructs. The expression and purification protocols have been optimised to allow for the preparation of soluble protein that will in future be used for crystallography and electron paramagnetic resonance (EPR) studies
Global research trends and hotspots of artificial intelligence research in spinal cord neural injury and restoration—a bibliometrics and visualization analysis
No full textBackgroundArtificial intelligence (AI) technology has made breakthroughs in spinal cord neural injury and restoration in recent years. It has a positive impact on clinical treatment. This study explores AI research’s progress and hotspots in spinal cord neural injury and restoration. It also analyzes research shortcomings related to this area and proposes potential solutions.MethodsWe used CiteSpace 6.1.R6 and VOSviewer 1.6.19 to research WOS articles on AI research in spinal cord neural injury and restoration.ResultsA total of 1,502 articles were screened, in which the United States dominated; Kadone, Hideki (13 articles, University of Tsukuba, JAPAN) was the author with the highest number of publications; ARCH PHYS MED REHAB (IF = 4.3) was the most cited journal, and topics included molecular biology, immunology, neurology, sports, among other related areas.ConclusionWe pinpointed three research hotspots for AI research in spinal cord neural injury and restoration: (1) intelligent robots and limb exoskeletons to assist rehabilitation training; (2) brain-computer interfaces; and (3) neuromodulation and noninvasive electrical stimulation. In addition, many new hotspots were discussed: (1) starting with image segmentation models based on convolutional neural networks; (2) the use of AI to fabricate polymeric biomaterials to provide the microenvironment required for neural stem cell-derived neural network tissues; (3) AI survival prediction tools, and transcription factor regulatory networks in the field of genetics were discussed. Although AI research in spinal cord neural injury and restoration has many benefits, the technology has several limitations (data and ethical issues). The data-gathering problem should be addressed in future research, which requires a significant sample of quality clinical data to build valid AI models. At the same time, research on genomics and other mechanisms in this field is fragile. In the future, machine learning techniques, such as AI survival prediction tools and transcription factor regulatory networks, can be utilized for studies related to the up-regulation of regeneration-related genes and the production of structural proteins for axonal growth
Structure of the archaeal Cascade subunit Csa5 : Relating the small subunits of CRISPR effector complexes
No full textThis work was funded by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC) (REF: BB/G011400/1) to M.F.W. and J.H.N. and a BBSRC-funded studentship to J.R.The Cascade complex for CRISPR-mediated antiviral immunity uses CRISPR RNA (crRNA) to target invading DNA species from mobile elements such as viruses, leading to their destruction. The core of the Cascade effector complex consists of the Cas5 and Cas7 subunits, which are widely conserved in prokaryotes. Cas7 binds crRNA and forms the helical backbone of Cascade. Many archaea encode a version of the Cascade complex (denoted Type I-A) that includes a Csa5 (or small) subunit, which interacts weakly with the core proteins. Here, we report the crystal structure of the Csa5 protein from Sulfolobus solfataricus. Csa5 comprises a conserved α-helical domain with a small insertion consisting of a weakly conserved β-strand domain. In the crystal, the Csa5 monomers have multimerized into infinite helical threads. At each interface is a strictly conserved intersubunit salt bridge, deletion of which disrupts multimerization. Structural analysis indicates a shared evolutionary history among the small subunits of the CRISPR effector complexes. The same α-helical domain is found in the C-terminal domain of Cse2 (from Type I-E Cascade), while the N-terminal domain of Cse2 is found in Cmr5 of the CMR (Type III-B) effector complex. As Cmr5 shares no match with Csa5, two possibilities present themselves: selective domain loss from an ancestral Cse2 to create two new subfamilies or domain fusion of two separate families to create a new Cse2 family. A definitive answer awaits structural studies of further small subunits from other CRISPR effector complexes.Peer reviewe
Enhanced removal of sulfonamide antibiotics by KOH-activated anthracite coal: Batch and fixed-bed studies
No full textThe presence of sulfonamide antibiotics in aquatic environments poses potential risks to human health and ecosystems. In the present study, a highly porous activated carbon was prepared by KOH activation of an anthracite coal (Anth-KOH), and its adsorption properties toward two sulfonamides (sulfamethoxazole and sulfapyridine) and three smaller-sized monoaromatics (phenol, 4-nitrophenol and 1,3-dinitrobenzene) were examined in both batch and fixed-bed adsorption experiments to probe the interplay between adsorbate molecular size and adsorbent pore structure. A commercial powder microporous activated carbon (PAC) and a commercial mesoporous carbon (CMK-3) possessing distinct pore properties were included as comparative adsorbents. Among the three adsorbents Anth-KOH exhibited the largest adsorption capacities for all test adsorbates (especially the two sulfonamides) in both batch mode and fixed-bed mode. After being normalized by the adsorbent surface area, the batch adsorption isotherms of sulfonamides on PAC and Anth-KOH were displaced upward relative to the isotherms on CMK-3, likely due to the micropore-filling effect facilitated by the microporosity of adsorbents. In the fixed-bed mode, the surface area-normalized adsorption capacities of Anth-KOH for sulfonamides were close to that of CMK-3, and higher than that of PAC. The irregular, closed micropores of PAC might impede the diffusion of the relatively large-sized sulfonamide molecules and in turn led to lowered fixed-bed adsorption capacities. The overall superior adsorption of sulfonamides on Anth-KOH can be attributed to its large specific surface area (2514 m(2)/g), high pore volume (1.23 cm(3)/g) and large micropore sizes (centered at 2.0 nm). These findings imply that KOH-activated anthracite coal is a promising adsorbent for the removal of sulfonamide antibiotics from aqueous solution. (C) 2016 Elsevier Ltd. All rights reserved.National Key Basic Research Program of China [2014CB441103]; National Natural Science Foundation of China [21225729, 21428701]SCI(E)[email protected]
