67,235 research outputs found
The prodomain restricts Neto activities.
No full text<p>(A) Diagram and Western blot analysis of constitutively active (CA) and processing mutant (PM) Neto-GFP variants. (B) Fluorescence images of salivary glands from third instar larvae expressing Neto-GFP variants as indicated (<i>G14>neto-GFP</i>). Prodomain processing did not affect the apical localization of Neto. (C) Co-immunoprecipitation (IP: α-GFP, WB: α-GluRIIC) from muscle extracts from control (<i>y<sup>1</sup>w<sup>1118</sup></i>) and <i>neto</i> rescued larvae (<i>neto<sup>36</sup>;G14>CA-neto-GFP</i> and <i>neto<sup>36</sup>;G14>PM-neto-GFP</i>) showed that both CA- and PM-Neto bind iGluRs. (D-E) PM-Neto is less efficient in rescuing the embryonic lethality and adult viability of <i>neto</i> null mutants compared with control or CA-Neto. Genotypes: control (<i>neto<sup>36</sup>;G14>neto-GFP</i> at 25°C); CA-Neto normal (<i>neto<sup>36</sup>;G14>CA-neto-GFP-N4</i> at 18°C); CA-Neto high (<i>neto<sup>36</sup>;G14>CA-neto-GFP-N4</i> at 25°C); PM-Neto normal (<i>neto<sup>36</sup>;G14>PM-neto-GFP-D2</i> at 25°C); PM-Neto high (<i>neto<sup>36</sup>;G14>PM-neto-GFP-D1</i> at 25°C). The numbers of animals analyzed are indicated in each bar. Bars: 10 μm.</p
Measurement of associated W+ charm production in pp collisions at √s=7 TeV
No full textOpen Access, Copyright CERN, for the benefit of the CMS Collaboration.Measurements are presented of the associated production of a W boson and a charm-quark jet (W + c) in pp collisions at a center-of-mass energy of 7 TeV. The analysis is conducted with a data sample corresponding to a total integrated luminosity of 5 fb−1, collected by the CMS detector at the LHC. W boson candidates are identified by their decay into a charged lepton (muon or electron) and a neutrino. The W + c measurements are performed for charm-quark jets in the kinematic region p jet T > 25 GeV, |ηjet| 25 GeV) and σ(pp → W + c + X)× B (W → ℓν) = 84.1 ± 2.0 (stat.) ± 4.9 (syst.) pb ( p ℓ T > 35 GeV), and the cross section ratios σ(pp → W+ + c ¯ + X)/σ(pp → W− + c + X) = 0.954 ± 0.025 (stat.) ± 0.004 (syst.) ( p ℓ T > 25 GeV) and σ(pp → W+ + c ¯ + X)/σ(pp → W− + c + X) = 0.938 ± 0.019 (stat.) ± 0.006 (syst.) ( p ℓ T > 35 GeV). Cross sections and cross section ratios are also measured differentially with respect to the absolute value of the pseudorapidity of the lepton from the W-boson decay. These are the first measurements from the LHC directly sensitive to the strange quark and antiquark content of the proton. Results are compared with theoretical predictions and are consistent with the predictions based on global fits of parton distribution functions
Neto-β is a novel Neto isoform at the <i>Drosophila</i> NMJ.
No full text<p>(A) Diagram of the <i>Drosophila neto</i> locus. Two isoforms are generated by alternative splicing, Neto-α and Neto-β. They have different cytoplasmic domains, but share highly conserved domains, CUB (complement <u>C</u>1r/C1s, <u>U</u>egf, <u>B</u>MP1), LDLa (LDL receptor class a) and transmembrane (TM), with Neto proteins from vertebrates and <i>C</i>. <i>elegans</i>. (B) Confocal images of third instar larvae NMJ4 from <i>neto</i><sup><i>null</i></sup> animals rescued with <i>neto</i>-<i>α</i> (<i>neto</i><sup><i>36</i></sup>;<i>G14>neto</i><sup><i>α</i></sup>), <i>neto</i>-<i>β</i> (<i>neto</i><sup><i>36</i></sup>;<i>G14>neto</i><sup>β</sup>), and a <i>neto</i> transgene lacking any intracellular part, <i>neto</i>Δ<sup>intra</sup><i>(neto</i><sup><i>36</i></sup>;<i>G14>neto</i>Δ<sup>intra</sup>). These Neto variants can rescue the viability and NMJ development in <i>neto</i><sup><i>null</i></sup> animals. The Neto-ex signals mark all NMJs, but the Neto-β antibodies specifically label control and <i>neto-β</i> rescued NMJs. (C-D) Confocal images of late embryos ventral muscle fields (indicated in white) labeled for (C) Neto-ex (red), Neto-β (green), HRP (blue) and (D) GluRIIA (red), Neto-β (green), HRP (blue) indicating the presence of Neto-β in the early stages of the larvae development. Genotypes: control (<i>w</i><sup><i>1118</i></sup>); <i>neto</i><sup><i>36</i></sup>;<i>G14>neto</i><sup><i>α</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y; G14-Gal4/UAS-neto-A9</i>); <i>neto</i><sup><i>36</i></sup>;<i>G14>neto</i><sup>β</sup> (<i>neto</i><sup><i>36</i></sup>, <i>UAS-neto-B6/Y; G14-Gal4/+</i>); <i>neto</i><sup><i>36</i></sup>;<i>G14>neto</i>Δ<sup>intra</sup>(<i>neto</i><sup><i>36</i></sup><i>/Y; G14-Gal4/ UAS-neto</i>Δ<sup>intra</sup><i>-H4</i>). Scale bars: (B) 20 μm (C-D) 10 μm; 5 μm in details.</p
Neto-β, but not Neto-α, restores PAK recruitment and mEJP amplitude at <i>neto</i><sup><i>null</i></sup> NMJs.
No full text<p>(A) Confocal images of larval NMJ4 labeled for Neto-ex (red), PAK (green) and HRP (blue). Neto-β, but not Neto-α, restores PAK synaptic accumulation over a large range of concentrations tested. (B) Western blot comparison of Neto levels in muscle extracts from control (first lane), and <i>neto</i><sup><i>null</i></sup> larvae rescued with <i>neto-α</i> transgenes (low, medium, and high expression) (magenta gradient), or <i>neto-β</i> transgenes (low, medium, high, and very high expression)(blue gradient). Arrows indicate unprocessed and processed Neto-α (magenta) and Neto-β (blue). (B’)—low exposure. Tubulin was used as a loading control. (C-H) Electrophysiological recordings of <i>neto</i><sup><i>null</i></sup> NMJs rescued by various levels of Neto-α or Neto-β. Data are reported relative to controls matched by rearing at 18°C (empty bars) or 25°C (hatched bars). Representative traces of mEJPs and EJPs are shown in (C) and (F), respectively. The mEJPs amplitude is reduced at NMJs rescued by low and medium levels of Neto-α (D). The mEJPs frequency appears less dependent on Neto levels/isoforms, but is significantly increased in larvae reared at 18°C (E). The EJPs amplitudes are largely normal (G), likely due to subtle, but significant increases in quantal content at Neto-α-rescued NMJs (H). (I) Confocal images of NMJ4 boutons (segment A3) in control (<i>w</i><sup><i>1118</i></sup>) and <i>GluRIIA</i><sup><i>SP16/Df</i></sup> (<i>GluRIIA</i><sup><i>SP16</i></sup>/ <i>Df(2L)cl</i><sup><i>h4</i></sup>) third instar larvae labeled for Neto-ex (red), PAK (green), and GluRIIA (blue). PAK is normally present at <i>GluRIIA</i> mutant synapses, indicating that the synaptic recruitment PAK does not depend on GluRIIA. Genotypes: control (<i>G14-Gal4/+</i>); <i>neto</i><sup><i>α low</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y</i>; <i>G14-Gal4/UAS-neto-A9</i>), reared at 25°C); <i>neto</i><sup><i>α med</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y</i>; <i>G14-Gal4/UAS-neto-A3</i>, 18°C); <i>neto</i><sup><i>α high</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y</i>; <i>G14-Gal4/UAS-neto-A3</i>, 25°C); <i>neto</i><sup><i>β low</i></sup> (<i>neto</i><sup><i>36</i></sup>, <i>UAS-neto-B6/Y</i>; <i>G14-Gal4/+</i>, 18°C); <i>neto</i><sup><i>β med</i></sup> (<i>neto</i><sup><i>36</i></sup>, <i>UAS-neto-B6</i>/Y; <i>G14-Gal4/+</i>, 25°C); <i>neto</i><sup><i>β high</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y</i>; <i>G14-Gal4/UAS-neto-B3</i>, 18°C); <i>neto</i><sup><i>β very high</i></sup> (<i>neto</i><sup><i>36</i></sup><i>/Y</i>; <i>G14-Gal4/UAS-neto-B3</i>, 25°C). Error bars indicate SEM. ***; p<0.001, **; p<0.005, *; p<0.05, ns; p>0.05. Scale bars: 20 μm, 2 μm in details and (I).</p
Correspondence and Photograph from Ernest W. Wright to Dr. C. A. Bacote, August 30, 1977
No full textCorrespondence between Ernest W. Wright (Employment Security Agency, Georgia Department of Labor) and Dr. C. A. Bacote, 30 August 1977. Enclosed 8"x10" photograph circa 1955, depicting voter registration participants. Written on Verso: "Left to Right: (Seated): Dr. Clarence A. Bacote, Dr. William Hale, Mr. Ron Harris? Dr. Robert Brisbane (Standing): Mr. Michael? Mr. C. A. Scott? Mr. Milton White"
Faust : Romance Of The Secret Tribunals / By George W. M. Reynolds, Author Of "The Mysteries Of London", "Pickwick Abroad", &c. Illustrated In The First Style Of The Art
No full textFAUST : ROMANCE OF THE SECRET TRIBUNALS / BY GEORGE W. M. REYNOLDS, AUTHOR OF "THE MYSTERIES OF LONDON", "PICKWICK ABROAD", &C. ILLUSTRATED IN THE FIRST STYLE OF THE ART
Faust : Romance Of The Secret Tribunals / By George W. M. Reynolds, Author Of "The Mysteries Of London", "Pickwick Abroad", &c. Illustrated In The First Style Of The Art (1)
Cover (1)
Umschlagtitel (8)
Titelseite (9)
Contents (10)
Illustrations / Prologue (11)
Prologue (12)
Chapter I.-XCV. (14)
Epilogue (119
Letter from C. W. Pence, Colonel, 442nd Infantry Regiment, Commanding, Headquarters, 442nd Combat Team, to Kiku Saito, May 20, 1943
No full textA letter from C. W. Pence, Colonel, 442nd Infantry Regiment to Kiku Saito incarcerated in the Granada camp, Colorado. It informs her of her son, Minoru Frank Saito's arrival at Camp Shelby, Mississippi. It congratulates her for his enlistment and quoted the President Roosevelt's statement, "Americanism is not, and never was, a matter of race or ancestry. A good American is one who is loyal to this country and to our creed of liberty and democracy.
Correspondence, C. W. Tayleure to John Brown, Jr., June 18, 1879
No full textA letter to C. W. Tayleure to John Brown, Jr. concerning the death of Brown's brother, Watson. 5 pages
Ophiothrix tommasi Santana & Manso & Almeida & Alves 2020, n. sp.
No full textOphiothrix tommasi n. sp. MZUSP 02617 (01 specimen), Piauí, Brazil, 02º49'S, 38º50'W, 72 m, coll. VIII/65 by Comis. Canopus; MZUSP 01542 (01 specimen), Ceará, Brazil, 02º49'S, 38º50'W, 72 m, coll. VIII/1965 by L. R. Comissão Canopus; MZUSP 02604 (02 specimens), Ceará, Brasil, 04°02'S, 35°84'W, 78 m, coll. 05/ VI/1998; LIMCE 00129 (01 specimen), Natal, Rio Grande do Norte, Brazil, 05°44'S, 35°12'W, coll. 14/ V/2003; MZUSP 01537 (01 specimen), Natal, Rio Grande do Norte, Brazil, 05º45'S, 34º58'W, 95 m, coll. I/1966 by Comissão Canopus; MZUSP 01539 (01 specimen), João Pessoa, Paraíba, Brazil, 07º08'S, 34º26'W, 62 m, coll. XII/1965 by Comissão Canopus; MZUSP 01536 (01 specimen), Fernando de Noronha, Pernambuco, Brazil, 04º02'S, 35º84'W, 78 m, coll. VI/1998 by Revizee N/NE; MZUSP 01538 (01 specimen), Pernambuco, Brazil, 07º50'S, 37º30'W, 67 m, coll. XII/1965 by Comissão Canopus; MZUSP 02609 (04 specimens), Pernambuco, Brazil, 07º08'– 07º50'S, 34º26'– 34º30'W, 62–67 m, coll. XII/1965by Comis. Canopus; MOUFPE-ECH 00086 (02 specimens), Itamaracá, Pernambuco, Brazil, 07°45'S, 34°38'W, coll. S. Souza, 28/XIII/1988; MOUFPE-ECH 00056 (04 specimens), Itamaracá, Pernambuco, Brazil, 07°45'S, 34°38'W, coll. S. Souza, 23/XI/1988; MOUFPE-ECH 00063 (02 specimens), Itamaracá, Pernambuco, Brazil, 07°45'S, 34°38'W, coll. 15/X/1993; MOUFPE-ECH 00070 (02 specimens), Tamandaré, Pernambuco, Brazil, 08°45'S, 35°05'W, coll. 03/ VI/2016; MOUFPE-ECH 00002 (01 specimen), MOUFPE-ECH 00044 (03 speci- mens), MOUFPE-ECH 00049 (01 specimen), Pernambuco, Brazil, 08°43'S, 35°05'W; MZUSP 01534 (01 specimen), Maceió, Alagoas, 09°34'– 09º53'S, 35º36'– 35º49'W, coll. 1966; UFBA 00901 (01 specimen), UFBA 00409 (06 specimens), Salvador, Bahia, Brazil, 12º57'S, 38º21'W, 0 m, coll. VII/2006 by W. Magalhães; MZUSP 02966 (01 specimen), Ilha Trindade, Espírito Santo, Brazil, 20º30'S, 40º18'W, 37 m, coll. V/1987; MZUSP 02967 (05 specimens), Ilha Trindade, Espírito Santo, Brazil, 20º29'S, 29º20'W, 13–15 m, XI/2014 by J.B. Mendonça; MZUSP 2601 (19 specimens), Bacia de Campos, Rio de Janeiro, 21°20'S, 40°35'W, 27 m, coll. 27/VII/1991 by L. R. Tommasi; MZUSP 2613 (01 specimen), Bacia de Campos, Rio de Janeiro, 21°20'S, 40°35'W, 57 m, coll. VII/1991 by L. R. Tommasi; MZUSP 02603 (02 specimens), Bacia de Campos, Rio de Janeiro, Brasil, 23°53'S, 42°28'W, 500 m, coll. 03/IX/1970 by L. R. Tommasi; MZUSP 01532 (01 specimen), MZUSP 01533 (01 specimen), Bacia de Campos, Rio de Janeiro, Brazil, 21°00'– 22º50'S, 40°00'– 41º58'W, 0–16 m, coll. 1957–2010; MZUSP 01540 (01 specimen), MZUSP 01541 (01 specimen), MZUSP 02968 (01 specimen), Búzios, Rio de Janeiro, Brazil, 22º49'S, 41º57'W, 16 m, coll. XI/1957 by L. R. Tommasi; MZUSP 02969 (01 specimen), MZUSP 01530 (01 specimen), MZUSP 01531 (03 specimens), Ubatuba, São Paulo, Brazil, 23º26'– 25º11'S, 44º57'– 45º02'W, coll. VIII/1956 by L. R. Tommasi; MZUSP 01543 (01 specimen), São Sebastião, São Paulo, Brazil, 23º44'S, 45º44'W, coll. IV/2013 by M. Tavares & J. Braga; MZUSP 02598 (01 specimen), Ilha Arvoredo, Santa Catarina, Brazil, 27°18'S; 48°23'W, 7,5 m, coll. 20/X/2004; MZUSP 02970 (01 specimen), Santa Catarina, Brazil, 27º43'S; 48º37'W, coll. 30/X/1959 by Tommasi.Published as part of Santana, Alisson, Manso, Cynthia L. C., Almeida, Ana C. S. & Alves, Orane F. S., 2020, Taxonomic review of Ophiothrix Müller & Troschel, 1840 (Echinodermata Ophiuroidea) from Brazil, with the description of four new species, pp. 51-78 in Zootaxa 4808 (1) on page 77, DOI: 10.11646/zootaxa.4808.1.3, http://zenodo.org/record/392790
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